{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:18:38Z","timestamp":1792790318499,"version":"4.5.0"},"posted":{"date-parts":[[2026,9,29]]},"group-title":"Immunology","reference-count":101,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"name":"NIH Common Fund","award":["1R35GM160483"],"award-info":[{"award-number":["1R35GM160483"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH Common Fund","award":["1F32HL182156"],"award-info":[{"award-number":["1F32HL182156"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH Common Fund","award":["P30CA023108"],"award-info":[{"award-number":["P30CA023108"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH Common Fund","award":["5P30CA023108"],"award-info":[{"award-number":["5P30CA023108"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH Common Fund","award":["1S10OD030242"],"award-info":[{"award-number":["1S10OD030242"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"crossref"}]},{"name":"NIH Common Fund","award":["P20GM130454"],"award-info":[{"award-number":["P20GM130454"]}],"id":[{"id":"https:\/\/ror.org\/001d55x84","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                <jats:p>Hematopoiesis sustains lifelong blood production and immunity and is strongly influenced by biological sex. Males and females differ in their hematopoietic composition and susceptibility to infection and hematologic disease. Because current models cannot readily disentangle the independent contributions of sex chromosomes versus hormones, the mechanisms underlying sexual dimorphism remain poorly understood. Zebrafish have proven to be a powerful, translatable system for studying hematopoiesis, and offer a unique opportunity to isolate hormonal contributions due to their absence of sex chromosomes. However, whether sex is regulated in the zebrafish hematopoietic system remained unknown. Here, we discovered pronounced sexual dimorphism in the adult zebrafish marrow, with compositional differences peaking at reproductive age. Transcriptome analysis of marrows revealed conservation of sex-specific programs between zebrafish and human datasets. Upon immune challenge with lipopolysaccharide, all major blood lineages exhibited sex-biased changes in their composition and transcriptomes with the largest responses in erythroid and myeloid cell types. Among these sex differences was an unexpected immunoregulation of the erythroid lineage across maturation states. These findings establish biological sex as a major regulator of zebrafish hematopoiesis and immunity, paralleling humans, and lay the groundwork necessary for future mechanistic studies on sexual dimorphism in hematologic health and disease.<\/jats:p>","DOI":"10.64898\/2026.09.24.754168","type":"posted-content","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:40:12Z","timestamp":1790750412000},"source":"Crossref","is-referenced-by-count":0,"title":["Sexual dimorphism in adult zebrafish marrows regulates blood cell composition and immune response"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7659-0612","authenticated-orcid":false,"given":"Shannon E.","family":"Paquette","sequence":"first","affiliation":[{"name":"Geisel School of Medicine, Dartmouth College, Hanover, NH, United States 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0140-2426","authenticated-orcid":false,"given":"Carolyn L.","family":"Winston","sequence":"additional","affiliation":[{"name":"Geisel School of Medicine, Dartmouth College, Hanover, NH, United States 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7006-3471","authenticated-orcid":false,"given":"Elizabeth A.","family":"Jones","sequence":"additional","affiliation":[{"name":"Geisel School of Medicine, Dartmouth College, Hanover, NH, United States 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3964-6680","authenticated-orcid":false,"given":"Dionna M.","family":"Kasper","sequence":"additional","affiliation":[{"name":"Geisel School of Medicine, Dartmouth College, Hanover, NH, United States 03755"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100312550475000_2026.09.24.754168v1.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.12.5382"},{"key":"2026100312550475000_2026.09.24.754168v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41374-018-0137-1"},{"key":"2026100312550475000_2026.09.24.754168v1.3","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1093\/gerona\/glab330","article-title":"Age-Related Sexual Dimorphism on the Longitudinal Progression of Blood Immune Cells in BALB\/cByJ Mice","volume":"77","year":"2022","journal-title":"J. Gerontol. Ser. A"},{"key":"2026100312550475000_2026.09.24.754168v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nature12932"},{"key":"2026100312550475000_2026.09.24.754168v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-024-03197-9"},{"key":"2026100312550475000_2026.09.24.754168v1.6","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.147645"},{"key":"2026100312550475000_2026.09.24.754168v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704861"},{"key":"2026100312550475000_2026.09.24.754168v1.8","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-015-2314-2"},{"key":"2026100312550475000_2026.09.24.754168v1.9","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.90"},{"key":"2026100312550475000_2026.09.24.754168v1.10","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1002\/jcp.30503","article-title":"Human monocytes respond to lipopolysaccharide (LPS) stimulation in a sex-dependent manner","volume":"237","year":"2022","journal-title":"J. Cell. Physiol"},{"key":"2026100312550475000_2026.09.24.754168v1.11","doi-asserted-by":"publisher","DOI":"10.1126\/science.adx4381"},{"key":"2026100312550475000_2026.09.24.754168v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2015.02.002"},{"key":"2026100312550475000_2026.09.24.754168v1.13","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2008.071008"},{"key":"2026100312550475000_2026.09.24.754168v1.14","doi-asserted-by":"crossref","first-page":"1150","DOI":"10.1186\/s12967-024-05990-2","article-title":"Sex differences in disease: sex chromosome and immunity","volume":"22","year":"2024","journal-title":"J. Transl. Med"},{"key":"2026100312550475000_2026.09.24.754168v1.15","doi-asserted-by":"crossref","first-page":"204","DOI":"10.3390\/curroncol32040204","article-title":"Sex and Gender in Myeloid and Lymphoblastic Leukemias and Multiple Myeloma: From Molecular Mechanisms to Clinical Outcomes","volume":"32","year":"2025","journal-title":"Curr. Oncol"},{"key":"2026100312550475000_2026.09.24.754168v1.16","doi-asserted-by":"crossref","first-page":"102678","DOI":"10.1016\/j.stemcr.2025.102678","article-title":"A human induced pluripotent stem cell toolbox for studying sex chromosome effects","volume":"20","year":"2025","journal-title":"Stem Cell Rep"},{"key":"2026100312550475000_2026.09.24.754168v1.17","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.07.013"},{"key":"2026100312550475000_2026.09.24.754168v1.18","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-023-00787-w"},{"key":"2026100312550475000_2026.09.24.754168v1.19","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.23884"},{"key":"2026100312550475000_2026.09.24.754168v1.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2011.11.012"},{"key":"2026100312550475000_2026.09.24.754168v1.21","doi-asserted-by":"publisher","DOI":"10.1111\/imr.70107"},{"key":"2026100312550475000_2026.09.24.754168v1.22","doi-asserted-by":"publisher","DOI":"10.1159\/000133152"},{"key":"2026100312550475000_2026.09.24.754168v1.23","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.22-20-09005.2002"},{"key":"2026100312550475000_2026.09.24.754168v1.24","doi-asserted-by":"publisher","DOI":"10.2307\/1313631"},{"key":"2026100312550475000_2026.09.24.754168v1.25","first-page":"e312","article-title":"The zebrafish: A fintastic model for hematopoietic development and disease. WIREs Dev","volume":"7","year":"2018","journal-title":"Biol"},{"key":"2026100312550475000_2026.09.24.754168v1.26","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2016.024"},{"key":"2026100312550475000_2026.09.24.754168v1.27","doi-asserted-by":"publisher","DOI":"10.1038\/ni1007"},{"key":"2026100312550475000_2026.09.24.754168v1.28","doi-asserted-by":"publisher","DOI":"10.1038\/nature08738"},{"key":"2026100312550475000_2026.09.24.754168v1.29","doi-asserted-by":"publisher","DOI":"10.1242\/dev.083147"},{"key":"2026100312550475000_2026.09.24.754168v1.30","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.006791"},{"key":"2026100312550475000_2026.09.24.754168v1.31","doi-asserted-by":"publisher","DOI":"10.1387\/ijdb.093042ep"},{"key":"2026100312550475000_2026.09.24.754168v1.32","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1016\/j.bbadis.2018.12.015","article-title":"Zebrafish disease models in hematology: Highlights on biological and translational impact","volume":"1865","year":"2019","journal-title":"Biochim. Biophys. Acta BBA - Mol. Basis Dis"},{"key":"2026100312550475000_2026.09.24.754168v1.33","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-05-503177"},{"key":"2026100312550475000_2026.09.24.754168v1.34","doi-asserted-by":"publisher","DOI":"10.1038\/nature05883"},{"key":"2026100312550475000_2026.09.24.754168v1.35","doi-asserted-by":"crossref","first-page":"e33865","DOI":"10.1016\/j.heliyon.2024.e33865","article-title":"Zebrafish as a model organism for virus disease research: Current status and future directions","volume":"10","year":"2024","journal-title":"Heliyon"},{"key":"2026100312550475000_2026.09.24.754168v1.36","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2019.08.005"},{"key":"2026100312550475000_2026.09.24.754168v1.37","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080280"},{"key":"2026100312550475000_2026.09.24.754168v1.38","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408708102"},{"key":"2026100312550475000_2026.09.24.754168v1.39","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.138"},{"key":"2026100312550475000_2026.09.24.754168v1.40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603349103"},{"key":"2026100312550475000_2026.09.24.754168v1.41","doi-asserted-by":"publisher","DOI":"10.1242\/dev.008904"},{"key":"2026100312550475000_2026.09.24.754168v1.42","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.189"},{"key":"2026100312550475000_2026.09.24.754168v1.43","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00501"},{"key":"2026100312550475000_2026.09.24.754168v1.44","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.114.169284"},{"key":"2026100312550475000_2026.09.24.754168v1.45","first-page":"8","article-title":"Sexual determination in zebrafish","volume":"79","year":"2021","journal-title":"Cell. Mol. Life Sci. CMLS"},{"key":"2026100312550475000_2026.09.24.754168v1.46","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207943"},{"key":"2026100312550475000_2026.09.24.754168v1.47","doi-asserted-by":"crossref","first-page":"102810","DOI":"10.1016\/j.xpro.2023.102810","article-title":"Protocol for the analysis of hematopoietic lineages in the whole kidney marrow of adult zebrafish","volume":"5","year":"2024","journal-title":"STAR Protoc"},{"key":"2026100312550475000_2026.09.24.754168v1.48","doi-asserted-by":"publisher","DOI":"10.1093\/ilar.53.2.161"},{"key":"2026100312550475000_2026.09.24.754168v1.49","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026100312550475000_2026.09.24.754168v1.50","doi-asserted-by":"crossref","first-page":"e124928","DOI":"10.1172\/jci.insight.124928","article-title":"Human bone marrow assessment by single-cell RNA sequencing, mass cytometry, and flow cytometry","volume":"3","year":"2018","journal-title":"JCI Insight"},{"key":"2026100312550475000_2026.09.24.754168v1.51","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt703"},{"key":"2026100312550475000_2026.09.24.754168v1.52","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-04-570762"},{"key":"2026100312550475000_2026.09.24.754168v1.53","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20170976"},{"key":"2026100312550475000_2026.09.24.754168v1.54","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-01-0179"},{"key":"2026100312550475000_2026.09.24.754168v1.55","doi-asserted-by":"crossref","first-page":"604000","DOI":"10.3389\/fimmu.2020.604000","article-title":"Sexual Dimorphism in Innate Immunity: The Role of Sex Hormones and Epigenetics","volume":"11","year":"2021","journal-title":"Front. Immunol"},{"key":"2026100312550475000_2026.09.24.754168v1.56","doi-asserted-by":"crossref","first-page":"113628","DOI":"10.1016\/j.bbr.2021.113628","article-title":"Sex differences in behavior, response to LPS, and glucose homeostasis in middle-aged mice","volume":"418","year":"2022","journal-title":"Behav. Brain Res"},{"key":"2026100312550475000_2026.09.24.754168v1.57","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2018020156"},{"key":"2026100312550475000_2026.09.24.754168v1.58","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-68792-8"},{"key":"2026100312550475000_2026.09.24.754168v1.59","doi-asserted-by":"publisher","DOI":"10.1038\/srep41905"},{"key":"2026100312550475000_2026.09.24.754168v1.60","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934593"},{"key":"2026100312550475000_2026.09.24.754168v1.61","doi-asserted-by":"crossref","unstructured":"Warren, A. , Garrett, K. & Frame, L. A . Disparities in women\u2019s health and clinical considerations from a translational science perspective: A narrative review and framework for future directions. Womens Health 21, 17455057251399009 (2025).","DOI":"10.1177\/17455057251399009"},{"key":"2026100312550475000_2026.09.24.754168v1.62","doi-asserted-by":"crossref","first-page":"1315","DOI":"10.1111\/bjh.20044","article-title":"Are we ignoring sex differences in haematological malignancies? A call for improved reporting","volume":"206","year":"2025","journal-title":"Br. J. Haematol"},{"key":"2026100312550475000_2026.09.24.754168v1.63","unstructured":"NOT-OD-15-102: Consideration of Sex as a Biological Variable in NIH-funded Research. https:\/\/grants.nih.gov\/grants\/guide\/notice-files\/not-od-15-102.html."},{"key":"2026100312550475000_2026.09.24.754168v1.64","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20220038"},{"key":"2026100312550475000_2026.09.24.754168v1.65","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-08-236729"},{"key":"2026100312550475000_2026.09.24.754168v1.66","doi-asserted-by":"crossref","unstructured":"White, R. M. & Patton, E. E . Adult zebrafish as advanced models of human disease. Dis. Model. Mech. 16, dmm050351 (2023).","DOI":"10.1242\/dmm.050351"},{"key":"2026100312550475000_2026.09.24.754168v1.67","doi-asserted-by":"crossref","first-page":"620984","DOI":"10.3389\/fcell.2020.620984","article-title":"Modeling Inflammation in Zebrafish for the Development of Anti-inflammatory Drugs","volume":"8","year":"2021","journal-title":"Front. Cell Dev. Biol"},{"key":"2026100312550475000_2026.09.24.754168v1.68","unstructured":"Sex-biased gene expression across mammalian organ development and evolution. Science https:\/\/www.science.org\/doi\/10.1126\/science.adf1046."},{"key":"2026100312550475000_2026.09.24.754168v1.69","doi-asserted-by":"publisher","DOI":"10.1038\/srep41905"},{"key":"2026100312550475000_2026.09.24.754168v1.70","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-12-327403"},{"key":"2026100312550475000_2026.09.24.754168v1.71","doi-asserted-by":"publisher","DOI":"10.1182\/bloodadvances.2021006088"},{"key":"2026100312550475000_2026.09.24.754168v1.72","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-331199"},{"key":"2026100312550475000_2026.09.24.754168v1.73","doi-asserted-by":"publisher","DOI":"10.1038\/nature12675"},{"key":"2026100312550475000_2026.09.24.754168v1.74","doi-asserted-by":"crossref","first-page":"705197","DOI":"10.3389\/fimmu.2021.705197","article-title":"Sex Matters: Physiological Abundance of Immuno-Regulatory CD71+ Erythroid Cells Impair Immunity in Females","volume":"12","year":"2021","journal-title":"Front. Immunol"},{"key":"2026100312550475000_2026.09.24.754168v1.75","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2007-1692"},{"key":"2026100312550475000_2026.09.24.754168v1.76","doi-asserted-by":"publisher","DOI":"10.1093\/gerona\/glt154"},{"key":"2026100312550475000_2026.09.24.754168v1.77","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.583550"},{"key":"2026100312550475000_2026.09.24.754168v1.78","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1097\/BS9.0000000000000017","article-title":"Erythroblast island macrophages: recent discovery and future perspectives","volume":"1","year":"2019","journal-title":"Blood Sci"},{"key":"2026100312550475000_2026.09.24.754168v1.79","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1111\/j.1365-2141.1972.tb08798.x","article-title":"The Effects of Repeated. Doses of Bacterial Endotoxin on Erythropoiesis in the Normal and Splenectomized Mouse","volume":"22","year":"1972","journal-title":"Br. J. Haematol"},{"key":"2026100312550475000_2026.09.24.754168v1.80","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-06-856500"},{"key":"2026100312550475000_2026.09.24.754168v1.81","doi-asserted-by":"crossref","unstructured":"Kanevskiy, L. M. , Telford, W. G. , Sapozhnikov, A. M. & Kovalenko, E. I . Lipopolysaccharide induces IFN-\u03b3 production in human NK cells. Front. Immunol. 4, 11 (2013).","DOI":"10.3389\/fimmu.2013.00011"},{"key":"2026100312550475000_2026.09.24.754168v1.82","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-169X.1977.00171.x"},{"key":"2026100312550475000_2026.09.24.754168v1.83","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-10-052761"},{"key":"2026100312550475000_2026.09.24.754168v1.84","doi-asserted-by":"crossref","first-page":"1132","DOI":"10.1172\/JCI107651","article-title":"Bone marrow erythropoiesis in the anemia of infection, inflammation, and malignancy","volume":"53","year":"1974","journal-title":"J. Clin. Invest"},{"key":"2026100312550475000_2026.09.24.754168v1.85","doi-asserted-by":"publisher","DOI":"10.1177\/039463200802100310"},{"key":"2026100312550475000_2026.09.24.754168v1.86","doi-asserted-by":"crossref","first-page":"100104","DOI":"10.1016\/j.bvth.2025.100104","article-title":"Neutrophils are key modulators of sex differences in LPS-induced shock in mice","volume":"2","year":"2025","journal-title":"Blood Vessels Thromb. Hemost"},{"key":"2026100312550475000_2026.09.24.754168v1.87","first-page":"3314","article-title":"The Transport and Inactivation Kinetics of Bacterial Lipopolysaccharide Influence its Immunological Potency in vivo","volume":"187","year":"2011","journal-title":"J. Immunol. Baltim. Md 1950"},{"key":"2026100312550475000_2026.09.24.754168v1.88","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/s41597-022-01544-7","article-title":"Single-cell RNA-seq of primary bone marrow neutrophils from female and male adult mice","volume":"9","year":"2022","journal-title":"Sci. Data"},{"key":"2026100312550475000_2026.09.24.754168v1.89","first-page":"1","article-title":"Zebrafish: A Versatile Animal Model for Fertility Research","volume":"2016","year":"2016","journal-title":"BioMed Res. Int"},{"key":"2026100312550475000_2026.09.24.754168v1.90","doi-asserted-by":"crossref","first-page":"809967","DOI":"10.3389\/fnana.2021.809967","article-title":"From Genes to Behavior: The Question of Evolutionary Conservation and the Role of Ethology in the Analysis of the Zebrafish","volume":"15","year":"2021","journal-title":"Front. Neuroanat"},{"key":"2026100312550475000_2026.09.24.754168v1.91","doi-asserted-by":"publisher","DOI":"10.1172\/JCI125755"},{"key":"2026100312550475000_2026.09.24.754168v1.92","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003919"},{"key":"2026100312550475000_2026.09.24.754168v1.93","doi-asserted-by":"crossref","first-page":"7725","DOI":"10.1038\/s41467-023-43175-5","article-title":"Tamoxifen for the treatment of myeloproliferative neoplasms: A Phase II clinical trial and exploratory analysis","volume":"14","year":"2023","journal-title":"Nat. Commun"},{"key":"2026100312550475000_2026.09.24.754168v1.94","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2016.149559"},{"key":"2026100312550475000_2026.09.24.754168v1.95","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12348-6"},{"key":"2026100312550475000_2026.09.24.754168v1.96","doi-asserted-by":"crossref","first-page":"582614","DOI":"10.3389\/fendo.2021.582614","article-title":"Sex Differences in the Immune System Become Evident in the Perinatal Period in the Four Core Genotypes Mouse","volume":"12","year":"2021","journal-title":"Front. Endocrinol"},{"key":"2026100312550475000_2026.09.24.754168v1.97","doi-asserted-by":"crossref","unstructured":"E3 medium (for zebrafish embryos). Cold Spring Harb. Protoc. 2011, pdb.rec66449 (2011).","DOI":"10.1101\/pdb.rec066449"},{"key":"2026100312550475000_2026.09.24.754168v1.98","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.55792"},{"key":"2026100312550475000_2026.09.24.754168v1.99","doi-asserted-by":"publisher","DOI":"10.1038\/S41587-023-01767-Y"},{"key":"2026100312550475000_2026.09.24.754168v1.100","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.21105\/joss.01686","article-title":"Welcome to the Tidyverse","volume":"4","year":"2019","journal-title":"J. Open Source Softw"},{"key":"2026100312550475000_2026.09.24.754168v1.101","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cels.2019.03.003","article-title":"DoubletFinder: Doublet Detection in Single-Cell RNA Sequencing Data Using Artificial Nearest Neighbors","volume":"8","year":"2019","journal-title":"Cell Syst"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.24.754168","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T19:55:20Z","timestamp":1791057320000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.24.754168"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,29]]},"references-count":101,"URL":"https:\/\/doi.org\/10.64898\/2026.09.24.754168","relation":{},"subject":[],"published":{"date-parts":[[2026,9,29]]},"subtype":"preprint"}}