{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T07:49:56Z","timestamp":1791532196357,"version":"4.3.4"},"posted":{"date-parts":[[2026,9,29]]},"group-title":"Biophysics","reference-count":99,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:00:00Z","timestamp":1790640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,9,29]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Liquid-liquid phase separation is a vital and ubiquitous principle of subcellular organization. Several actin-binding proteins have recently been shown to undergo liquid-liquid phase separation, forming micron-sized droplets that assemble actin into distinct network shapes. However, our knowledge of how these phase-separated proteins organize actin filaments at the nanoscale is rather limited. Here, we seek to address this knowledge gap by investigating synapsin-1 condensates through a combination of computational simulations and nanoscale imaging experiments. Our results show that while synapsin-1 condensates by themselves lack any special structural organization, the addition of actin filaments results in the generation of a regularly organized actin scaffold within the condensates. The organized scaffold forms both when actin and synapsin-1 are added simultaneously and when actin is added after the formation of synapsin condensates. Dissolving the condensates, by synapsin-1 removal, leaves the actin scaffold largely unaffected in both simulations and experiments, implying that this scaffold should guide droplet reformation when synapsin-1 is added back. Taken together, our findings suggest that the actin-rich component of the condensate retains a structural memory that promotes and guides the reassembly of synapsin-actin condensates. This structural memory may be important in the long-term maintenance of synaptic function\n                  <jats:italic>in vivo<\/jats:italic>\n                  .\n                <\/jats:p>","DOI":"10.64898\/2026.09.25.754542","type":"posted-content","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:10:21Z","timestamp":1790745021000},"source":"Crossref","is-referenced-by-count":0,"title":["Synapsin-1 and actin form ordered nanoscale assemblies"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-0518-0407","authenticated-orcid":false,"given":"Daniel","family":"Mansour","sequence":"first","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajdeep","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Department of Neuro- and Sensory Physiology, University Medical Center G\u00f6ttingen, 37073 G\u00f6ttingen, Germany"},{"name":"Department of Chemistry, GITAM School of Science, GITAM, Hyderabad, Telangana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aravind","family":"Chandrasekaran","sequence":"additional","affiliation":[{"name":"Department of Mechanical and Aerospace Engineering, University of California San Diego, La Jolla, CA, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiago","family":"Mimoso","sequence":"additional","affiliation":[{"name":"Institute for X-Ray Physics, University of G\u00f6ttingen, Friedrich-Hund-Platz 1, 37077, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donatus","family":"Krah","sequence":"additional","affiliation":[{"name":"Department of Neuro- and Sensory Physiology, University Medical Center G\u00f6ttingen, 37073 G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandr","family":"Korobeinikov","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akshita","family":"Chhabra","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dragomir","family":"Milovanovic","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuroscience, German Center for Neurodegenerative Diseases (DZNE), 10117, Berlin, Germany"},{"name":"Institute of Biochemistry and Einstein Center for Neurosciences, Charit\u00e9-Universit\u00e4tsmedizin Berlin, Corporate Member of Freie Universit\u00e4t Berlin, Humboldt-Universit\u00e4t Berlin, and Berlin Institute of Health, 10117, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"K\u00f6ster","sequence":"additional","affiliation":[{"name":"Institute for X-Ray Physics, University of G\u00f6ttingen, Friedrich-Hund-Platz 1, 37077, G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali H.","family":"Shaib","sequence":"additional","affiliation":[{"name":"Department of Neuro- and Sensory Physiology, University Medical Center G\u00f6ttingen, 37073 G\u00f6ttingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio O.","family":"Rizzoli","sequence":"additional","affiliation":[{"name":"Department of Neuro- and Sensory Physiology, University Medical Center G\u00f6ttingen, 37073 G\u00f6ttingen, 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