{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:19:31Z","timestamp":1792790371077,"version":"4.5.0"},"posted":{"date-parts":[[2026,10,4]]},"group-title":"Immunology","reference-count":18,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T00:00:00Z","timestamp":1791072000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"award":["MH120327"],"award-info":[{"award-number":["MH120327"]}],"id":[{"id":"https:\/\/ror.org\/04xeg9z08","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Institute of Arthritis and Musculoskeletal and Skin Diseases","award":["AR078781"],"award-info":[{"award-number":["AR078781"]}],"id":[{"id":"https:\/\/ror.org\/006zn3t30","id-type":"ROR","asserted-by":"crossref"}]},{"award":["2022-08-051"],"award-info":[{"award-number":["2022-08-051"]}],"id":[{"id":"https:\/\/ror.org\/00her7k05","id-type":"ROR","asserted-by":"publisher"}]},{"award":["31308"],"award-info":[{"award-number":["31308"]}],"id":[{"id":"https:\/\/ror.org\/03a63f080","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Early-life adversity (ELA) is one of the strongest predictors of poor mental and physical health later in life, increasing an individual\u2019s risk for disorders ranging from depression to metabolic syndrome. However, whether and how ELA shapes immune memory through adulthood, subsequently affecting susceptibility to immune-associated diseases such as autoimmunity, remains elusive.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>To address this question, we employed a translationally and ethologically relevant mouse model of resource deprivation, the limited bedding and nesting model, to study the long-term effects of ELA on the immune system. ELA was induced by limited bedding and nesting material in the cage during postnatal days (P)2-9, which provokes fragmented and unpredictable maternal behavior in the dams, and results in chronic stress in the pups. Nucleic acid Linked ImmunoSandwich Assay (NULISA) was used to survey the proteome of adult (\u223cP90) mice of both sexes that experienced ELA vs. control conditions.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>Using a novel proteomic method, we detected significant upregulation in the levels of IL-17A in the adult blood, primarily in females. Within females, ELA also upregulated IL-13 and CCL3. Three proteins, HGF, PDGFA, and WNT16, were significantly downregulated in ELA mice of both sexes.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Being a hallmark proinflammatory cytokine, IL-17A is found to increase in patients with systemic lupus erythematosus (SLE), as well as in animals with lupus-like diseases in virtually all reports. Importantly, autoimmune diseases like SLE have a higher prevalence in women than men, and our data support this sex bias. Together, our data suggest that ELA shapes long-term immune function in a way that may increase risk for autoimmune diseases, with promise for the development of more targeted and personalized therapies for autoimmunity.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.09.28.755115","type":"posted-content","created":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:15:14Z","timestamp":1791130514000},"source":"Crossref","is-referenced-by-count":0,"title":["Early-life adversity alters the adult blood proteome primarily in female mice"],"prefix":"10.64898","author":[{"given":"Madison M.","family":"Garvin","sequence":"first","affiliation":[{"name":"Neuroscience Institute, Georgia State University, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ae Ra","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Physiology, Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8872-4999","authenticated-orcid":false,"given":"Jessica L.","family":"Bolton","sequence":"additional","affiliation":[{"name":"Neuroscience Institute, Georgia State University, Atlanta, GA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Department of Pharmacology and Toxicology, Michigan State University, East Lansing, MI, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100810002188000_2026.09.28.755115v1.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neubiorev.2023.105113"},{"key":"2026100810002188000_2026.09.28.755115v1.2","first-page":"642","article-title":"Childhood trauma and adulthood inflammation: a meta-analysis of peripheral C-reactive protein, interleukin-6 and tumour necrosis factor-\u03b1","volume":"21","year":"2015","journal-title":"Molecular Psychiatry 2016 21:5"},{"key":"2026100810002188000_2026.09.28.755115v1.3","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1001\/2013.jamapsychiatry.4","article-title":"A Randomized Controlled Trial of the Tumor Necrosis Factor Antagonist Infliximab for Treatment-Resistant Depression: The Role of Baseline Inflammatory Biomarkers","volume":"70","year":"2013","journal-title":"JAMA Psychiatry"},{"key":"2026100810002188000_2026.09.28.755115v1.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.110600"},{"key":"2026100810002188000_2026.09.28.755115v1.5","first-page":"248","article-title":"The ABCs of autoimmune disease","volume":"14","year":"2018","journal-title":"Nature Reviews Rheumatology 2018 14:5"},{"key":"2026100810002188000_2026.09.28.755115v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2011.10.022"},{"key":"2026100810002188000_2026.09.28.755115v1.7","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2014.23"},{"key":"2026100810002188000_2026.09.28.755115v1.8","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.190009"},{"key":"2026100810002188000_2026.09.28.755115v1.9","doi-asserted-by":"crossref","unstructured":"K\u00f3sa, F. et al. High risk of depression, anxiety, and an unfavorable complex comorbidity profile is associated with SLE: a nationwide patient-level study. Arthritis Res. 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