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Single cell genomics in &gt;1.7 million single cells across donors with the cancer predisposition syndrome neurofibromatosis type 1 (NF-1), shows that somatic \u2018second-hit\u2019 mutations in\n                  <jats:italic>NF1<\/jats:italic>\n                  are pervasive and show positive clonal selection in oligodendrocytes (OLs) and OL precursor cells (OPCs), but are exceedingly rare in neurons and other lineages. Combined spatial genotyping and transcriptomic analysis enabled dissection of cell autonomous and non-autonomous effects of second-hit mutations including clonal expansion of mutant OPCs with strong bias for cerebral gray matter versus white matter, suggesting that gray matter influences OPC proliferation and differentiation via non-cell autonomous interactions between OPCs and neurons.\n                  <jats:italic>NF1<\/jats:italic>\n                  -null OL lineage cells upregulate\n                  <jats:italic>CDKN2A<\/jats:italic>\n                  \u2014a tumor suppressor frequently lost in\n                  <jats:italic>NF1<\/jats:italic>\n                  -mutant tumors\u2014 whereas brain cancers from these same individuals show\n                  <jats:italic>CDKN2A<\/jats:italic>\n                  loss across multiple cancer cell states, suggesting\n                  <jats:italic>CDKN2A<\/jats:italic>\n                  loss in OPCs is a critical step in tumor progression. Our findings reveal developmentally early, exquisitely lineage-specific\n                  <jats:italic>NF1<\/jats:italic>\n                  somatic mutations in premalignant NF-1 brain, suggest cell autonomous and non-autonomous roles in clonal selection in brain, and suggest that clones harboring cancer driver mutations can persist over a lifetime in phenotypically normal tissues.\n                <\/jats:p>","DOI":"10.64898\/2026.09.29.755447","type":"posted-content","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T03:55:15Z","timestamp":1790826915000},"source":"Crossref","is-referenced-by-count":0,"title":["Pervasive Somatic Mutations in\n                  <i>NF1<\/i>\n                  Drive Cell Type and Spatially Specific Clonal Selection in Neurofibromatosis"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0615-9111","authenticated-orcid":false,"given":"Daniel A.","family":"Snellings","sequence":"first","affiliation":[{"name":"Division of 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