{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:53:24Z","timestamp":1791312804633,"version":"4.3.1"},"posted":{"date-parts":[[2026,10,2]]},"group-title":"Developmental Biology","reference-count":58,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["R01AR077238"],"award-info":[{"award-number":["R01AR077238"]}],"id":[{"id":"https:\/\/ror.org\/01cwqze88","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Human hair follicle engineering is hindered by the rapid loss of inductive competence in culture-expanded dermal papilla (DP) fibroblasts. Here we show that bone morphogenetic protein 6 (BMP6) maintains and restores DP-associated characteristics while promoting follicular epithelial differentiation by restricting keratinocyte (KC) proliferation and inducing hair follicle-associated marker expression. Human follicle transcriptomics further linked BMP activity to DP identity and follicular epithelial differentiation. Genetic mouse studies demonstrate that BMP6 in Sox2-positive DP cells contributes to normal anagen onset, progression, and maintenance. Gain- and loss-of-function studies in KC\u2013DP organoids show that BMP6 promotes epithelial\u2013 mesenchymal organization and spatially patterned follicular structures.\n                  <jats:italic>In vivo<\/jats:italic>\n                  , topical BMP6 promotes anagen onset, whereas\n                  <jats:italic>BMP6<\/jats:italic>\n                  overexpression restores the hair follicle-generating capacity of late-passage human DP fibroblasts. Together, these findings identify BMP6 as a compartment-dependent regulator that enhances human DP competence and promotes epithelial differentiation, supporting regenerative hair follicle formation and engineering with culture-expanded DP cells.\n                <\/jats:p>","DOI":"10.64898\/2026.09.30.755211","type":"posted-content","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T08:15:18Z","timestamp":1790928918000},"source":"Crossref","is-referenced-by-count":0,"title":["BMP6 coordinates dermal papilla and epithelial cell states for hair follicle engineering and regeneration"],"prefix":"10.64898","author":[{"given":"Lindsey G.","family":"Siegfried","sequence":"first","affiliation":[{"name":"Division of Pharmaceutical Sciences, University of Cincinnati College of Pharmacy, Cincinnati, OH, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linli","family":"Zhou","sequence":"additional","affiliation":[{"name":"Division of Pharmaceutical Sciences, 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