{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:57:04Z","timestamp":1791302224960,"version":"4.1.0"},"posted":{"date-parts":[[2026,10,1]]},"group-title":"Molecular Biology","reference-count":30,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institutes of Health"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>Simulated microgravity provides a controlled perturbation for testing how loss of mechanical load affects human tissues. After 24 hours in rotating-wall vessels, neural, cardiac and tonsil organoids developed tissue-specific transcriptional responses that overlapped published spaceflight datasets, accompanied by higher transcriptomic-age estimates in neural and cardiac models, altered cardiac-organoid contraction dynamics and modified tonsil-organoid antiviral gene-expression signatures. These findings establish a multi-organoid framework for studying acute responses to gravitational unloading.<\/jats:p>","DOI":"10.64898\/2026.09.30.755771","type":"posted-content","created":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:10:14Z","timestamp":1790914214000},"source":"Crossref","is-referenced-by-count":0,"title":["Simulated microgravity induces aging phenotypes in human organoids"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2669-7312","authenticated-orcid":false,"given":"Wei-Chieh","family":"Mu","sequence":"first","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4498-6647","authenticated-orcid":false,"given":"Jordan","family":"Baechle","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-8046-0167","authenticated-orcid":false,"given":"Kevin","family":"Schneider","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7353-4394","authenticated-orcid":false,"given":"Matias","family":"Fuentealba","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8353-7676","authenticated-orcid":false,"given":"Fei","family":"Wu","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Manwaring-Mueller","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7938-1691","authenticated-orcid":false,"given":"Nathan","family":"Bracey","sequence":"additional","affiliation":[{"name":"Stanford School of Medicine, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-1567-865X","authenticated-orcid":false,"given":"Alexander","family":"Chebykin","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9576-0760","authenticated-orcid":false,"given":"Abhayjit","family":"Saini","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0045-1429","authenticated-orcid":false,"given":"Jiwoon","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Systems and Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8733-9925","authenticated-orcid":false,"given":"JangKeun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Systems and Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8702-0957","authenticated-orcid":false,"given":"Daniel A.","family":"Winer","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"},{"name":"Division of Cellular & Molecular Biology, Diabetes Research Group, Toronto General Hospital Research Institute (TGHRI), University Health Network, Toronto, ON, Canada"},{"name":"Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada"},{"name":"Department of Immunology, University of Toronto, Toronto, ON, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-5724-1650","authenticated-orcid":false,"given":"Alan I.","family":"Gale","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1850-1642","authenticated-orcid":false,"given":"Christopher E.","family":"Mason","sequence":"additional","affiliation":[{"name":"Department of Systems and Computational Biomedicine, Weill Cornell Medicine, New York, NY, USA"},{"name":"Trivedi Institute of Space and Global Biomedicine, University of Pittsburgh, Pittsburgh, PA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark M.","family":"Davis","sequence":"additional","affiliation":[{"name":"Stanford School of Medicine, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3654-9519","authenticated-orcid":false,"given":"David","family":"Furman","sequence":"additional","affiliation":[{"name":"Buck Institute for Research on Aging, Novato, CA, USA"},{"name":"Stanford School of Medicine, Stanford University, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"54368","reference":[{"key":"2026100608150896000_2026.09.30.755771v1.1","doi-asserted-by":"crossref","first-page":"1284644","DOI":"10.3389\/fphys.2024.1284644","volume":"15","year":"2024","journal-title":"Front. Physiol"},{"key":"2026100608150896000_2026.09.30.755771v1.2","doi-asserted-by":"crossref","unstructured":"Roy-O\u2019Reilly, M. , Mulavara, A. & Williams, T. NPJ Microgravity 7, 5 (2021). doi","DOI":"10.1038\/s41526-021-00133-z"},{"key":"2026100608150896000_2026.09.30.755771v1.3","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1093\/pcmedi\/pbaa022","volume":"3","year":"2020","journal-title":"Precis. Clin. Med"},{"key":"2026100608150896000_2026.09.30.755771v1.4","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01437"},{"key":"2026100608150896000_2026.09.30.755771v1.5","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1038\/s41586-024-07639-y","volume":"632","year":"2024","journal-title":"Nature"},{"key":"2026100608150896000_2026.09.30.755771v1.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.11.002"},{"key":"2026100608150896000_2026.09.30.755771v1.7","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.2001.281.1.F12"},{"key":"2026100608150896000_2026.09.30.755771v1.8","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2653"},{"key":"2026100608150896000_2026.09.30.755771v1.9","doi-asserted-by":"publisher","DOI":"10.1093\/stcltm\/szae070"},{"key":"2026100608150896000_2026.09.30.755771v1.10","doi-asserted-by":"crossref","first-page":"2272","DOI":"10.1016\/j.stemcr.2022.08.007","volume":"17","year":"2022","journal-title":"Stem Cell Reports"},{"key":"2026100608150896000_2026.09.30.755771v1.11","doi-asserted-by":"crossref","first-page":"4795","DOI":"10.1038\/s41467-023-42013-y","volume":"15","year":"2024","journal-title":"Nat. Commun"},{"key":"2026100608150896000_2026.09.30.755771v1.12","doi-asserted-by":"publisher","DOI":"10.1016\/J.CELL.2022.11.001"},{"key":"2026100608150896000_2026.09.30.755771v1.13","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw943"},{"key":"2026100608150896000_2026.09.30.755771v1.14","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-018-0829-3"},{"key":"2026100608150896000_2026.09.30.755771v1.15","doi-asserted-by":"crossref","first-page":"142","DOI":"10.3390\/biom12010142","volume":"12","year":"2022","journal-title":"Biomolecules"},{"key":"2026100608150896000_2026.09.30.755771v1.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-01145-0"},{"key":"2026100608150896000_2026.09.30.755771v1.17","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.stemcr.2019.09.006","volume":"13","year":"2019","journal-title":"Stem Cell Reports"},{"key":"2026100608150896000_2026.09.30.755771v1.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.07.003"},{"key":"2026100608150896000_2026.09.30.755771v1.19","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"2026100608150896000_2026.09.30.755771v1.20","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0201-4"},{"key":"2026100608150896000_2026.09.30.755771v1.21","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"2026100608150896000_2026.09.30.755771v1.22","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"2026100608150896000_2026.09.30.755771v1.23","doi-asserted-by":"publisher","DOI":"10.1089\/omi.2011.0118"},{"key":"2026100608150896000_2026.09.30.755771v1.24","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btac757"},{"key":"2026100608150896000_2026.09.30.755771v1.25","unstructured":"Korotkevich, G. et al. Preprint at bioRxiv (2016). doi"},{"key":"2026100608150896000_2026.09.30.755771v1.26","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-27903-2"},{"key":"2026100608150896000_2026.09.30.755771v1.27","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkq636"},{"key":"2026100608150896000_2026.09.30.755771v1.28","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-021-02533-6"},{"key":"2026100608150896000_2026.09.30.755771v1.29","first-page":"1","volume":"106","year":"2023","journal-title":"Stat. Softw"},{"key":"2026100608150896000_2026.09.30.755771v1.30","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-14-7"}],"container-title":[],"original-title":[],"link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.64898\/2026.09.30.755771","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:15:21Z","timestamp":1791299721000},"score":1,"resource":{"primary":{"URL":"http:\/\/biorxiv.org\/lookup\/doi\/10.64898\/2026.09.30.755771"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,1]]},"references-count":30,"URL":"https:\/\/doi.org\/10.64898\/2026.09.30.755771","relation":{},"subject":[],"published":{"date-parts":[[2026,10,1]]},"subtype":"preprint"}}