{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T18:01:54Z","timestamp":1774116114186,"version":"3.50.1"},"posted":{"date-parts":[[2026,3,18]]},"group-title":"Microbiology","reference-count":56,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:00:00Z","timestamp":1773792000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,3,18]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Fatty acids (FAs) are key metabolites in living organisms, shaping membrane architecture and helping cellular acclimatization to changing conditions. Bacteria synthesize FA\n                  <jats:italic>de novo<\/jats:italic>\n                  but can also reclaim them from membrane lipids or uptake them from the environment, usually converting free fatty acids (FFAs) into activated FAs that can then be further metabolized. In cyanobacteria, it is the acyl-acyl carrier protein synthetase (Aas) that activates exogenous FFAs. Yet, the cyanobacterial enzyme BrtB was recently shown to esterify,\n                  <jats:italic>in vitro<\/jats:italic>\n                  , FFAs directly onto abundant chlorinated glycolipids (bartolosides), generating bartoloside fatty acid esters (B-FAs). Whether this chemistry operates\n                  <jats:italic>in vivo<\/jats:italic>\n                  , where it occurs, and what its implications are for cell physiology has remained unclear. Here we show that in the cyanobacterium\n                  <jats:italic>Synechocystis salina<\/jats:italic>\n                  LEGE 06099, BrtB captures FFAs at the cell envelope without prior activation to generate B-FAs. We found that supplemented FFAs were converted into B-FAs within minutes. Strikingly, this response occurred with minimal changes in gene expression and little alteration of the extracellular proteome, consistent with a pathway already in place. Additionally, we observed that B-FAs can further be hydrolyzed into hydroxybartolosides \u2013 the levels of the latter metabolites increase in response to FA supply, suggesting a transient sequestration of FFA. Our results demonstrate that activation of FFA is not the only route to their cellular incorporation and identify a specialized-metabolite pathway that captures exogenous FFAs at the cyanobacterial cell envelope.\n                <\/jats:p>","DOI":"10.64898\/2026.03.17.712404","type":"posted-content","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T05:45:25Z","timestamp":1773899125000},"source":"Crossref","is-referenced-by-count":0,"title":["Activation-independent capture of free fatty acids at the bacterial cell envelope by BrtB"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8516-7296","authenticated-orcid":false,"given":"Amaranta","family":"Kahn","sequence":"first","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1160-2195","authenticated-orcid":false,"given":"Cristina I.F.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7769-7470","authenticated-orcid":false,"given":"Jo\u00e3o P. A.","family":"Reis","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4729-1701","authenticated-orcid":false,"given":"David A.","family":"Russo","sequence":"additional","affiliation":[{"name":"Bioorganic Analytics, Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9647-1197","authenticated-orcid":false,"given":"Adriana","family":"Rego","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]},{"given":"Ricardo J.C.V","family":"Queir\u00f3s","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1728-7901","authenticated-orcid":false,"given":"Marine","family":"Cuau","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0462-8810","authenticated-orcid":false,"given":"Julie A. Z.","family":"Zedler","sequence":"additional","affiliation":[{"name":"Synthetic Biology of Photosynthetic Organisms, Matthias Schleiden Institute for Genetics, Bioinformatics and Molecular Botany, Friedrich Schiller University Jena, 07743 Jena, Germany"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7949-0513","authenticated-orcid":false,"given":"Sandra A.C.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"},{"name":"Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Coimbra, 3000-548, Coimbra, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0938-152X","authenticated-orcid":false,"given":"Paulo","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"},{"name":"Department of Biology, Faculty of Sciences, University of Porto, R Campo Alegre, s\/n, 4169-007, Porto, Portugal"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5064-9164","authenticated-orcid":false,"given":"Pedro N.","family":"Le\u00e3o","sequence":"additional","affiliation":[{"name":"Interdisciplinary Centre of Marine and Environmental Research (CIIMAR\/CIMAR), University of Porto, Avenida General Norton de Matos, s\/n, 4450-208, Matosinhos, Portugal"}]}],"member":"54368","reference":[{"key":"2026032110550851000_2026.03.17.712404v1.1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.11.3607-3619.2005"},{"key":"2026032110550851000_2026.03.17.712404v1.2","doi-asserted-by":"publisher","DOI":"10.1038\/s41556-024-01364-4"},{"key":"2026032110550851000_2026.03.17.712404v1.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2016.05.002"},{"key":"2026032110550851000_2026.03.17.712404v1.4","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio834"},{"key":"2026032110550851000_2026.03.17.712404v1.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-53975-y"},{"key":"2026032110550851000_2026.03.17.712404v1.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-024-01464-7"},{"key":"2026032110550851000_2026.03.17.712404v1.7","doi-asserted-by":"publisher","DOI":"10.1039\/c4mb00443d"},{"key":"2026032110550851000_2026.03.17.712404v1.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.06.015"},{"key":"2026032110550851000_2026.03.17.712404v1.9","doi-asserted-by":"publisher","DOI":"10.1093\/FEMSRE\/FUAD020"},{"key":"2026032110550851000_2026.03.17.712404v1.10","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-74731-4"},{"key":"2026032110550851000_2026.03.17.712404v1.11","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.195263"},{"key":"2026032110550851000_2026.03.17.712404v1.12","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.148007"},{"key":"2026032110550851000_2026.03.17.712404v1.13","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1349-8"},{"key":"2026032110550851000_2026.03.17.712404v1.14","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201503186"},{"key":"2026032110550851000_2026.03.17.712404v1.15","doi-asserted-by":"crossref","unstructured":"Figueiredo, S. 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