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Understanding these drug\u2013microbiome interactions is important for a wide range of gut-related diseases, including colorectal cancer (CRC), and for the development of microbiome-based CRC biomarkers.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Objective<\/jats:title>\n                  <jats:p>To examine associations between prescription drug use, the gut microbiome and screening-detected colorectal lesions, and to investigate whether drug exposure and its timing influences microbial CRC screening biomarker candidates.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Design<\/jats:title>\n                  <jats:p>Associations of current, past and chronic prescription drug use with the gut microbiome and colorectal lesions were investigated in a cohort of 1,034 fecal immunochemical test-positive screening participants aged 55-77 years. Gut metagenomic profiles were combined with clinicopathologic, demographic, and lifestyle information, and linked to 12 years of prescription records from the national registry.<\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>\n                    Among the 83 drugs tested (ATC5), 38 were associated with microbial diversity and bacterial species. Of 168 drug-associated species (32.9% of all species), seven have previously been associated with colorectal neoplasia in the same cohort. Six CRC biomarker candidates showed positive associations with drug use:\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    with metformin;\n                    <jats:italic>Clostridium symbiosum<\/jats:italic>\n                    with codeine-paracetamol combination;\n                    <jats:italic>Eggerthella lenta<\/jats:italic>\n                    with clindamycin;\n                    <jats:italic>Flavonifractor plautii<\/jats:italic>\n                    with dicloxacillin, clindamycin, and codeine-paracetamol combination; and\n                    <jats:italic>Streptococcus parasanguinis<\/jats:italic>\n                    and\n                    <jats:italic>Streptococcus salivarius<\/jats:italic>\n                    with pantoprazole and esomeprazole. The unclassified Actinobacteria species GGB34797 SGB14322 was negatively associated with clindamycin.\n                  <\/jats:p>\n                <\/jats:sec>\n                <jats:sec>\n                  <jats:title>Conclusion<\/jats:title>\n                  <jats:p>These findings suggest that commonly used drugs, such as painkillers, metformin, antibiotics, and proton pump inhibitors, induce measurable changes in the gut microbiome that may influence microbial CRC screening biomarker candidates.<\/jats:p>\n                <\/jats:sec>","DOI":"10.64898\/2026.08.12.26360274","type":"posted-content","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T20:55:13Z","timestamp":1786654513000},"source":"Crossref","is-referenced-by-count":0,"title":["Metformin, antibiotics, painkillers, and proton pump inhibitors shape the gut microbiome, including microbial signatures associated with colorectal neoplasia"],"prefix":"10.64898","author":[{"given":"ES.","family":"Karstad","sequence":"first","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Bioscencies, Department of Pharmacy, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9361-7987","authenticated-orcid":false,"given":"E.","family":"Birkeland","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Bioscencies, Department of Pharmacy, University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9935-2804","authenticated-orcid":false,"given":"E.","family":"Avershina","sequence":"additional","affiliation":[{"name":"Section for Pharmacology and Pharmaceutical Bioscencies, Department of Pharmacy, University of Oslo, Oslo, Norway"},{"name":"Department of Tumour Biology, Institute of Cancer Research, Oslo University Hospital, Oslo, 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