{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"institution":[{"name":"bioRxiv"}],"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:51:19Z","timestamp":1791255079230,"version":"4.1.0"},"posted":{"date-parts":[[2026,10,1]]},"group-title":"Bioinformatics","reference-count":63,"publisher":"openRxiv","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.biorxiv.org\/about\/FAQ#license"}],"funder":[{"award":["P40 OD010965"],"award-info":[{"award-number":["P40 OD010965"]}],"id":[{"id":"https:\/\/ror.org\/01jdyfj45","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Institute on Aging","award":["P30 AG021332"],"award-info":[{"award-number":["P30 AG021332"]}],"id":[{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"crossref"}]},{"award":["R24 AG073199"],"award-info":[{"award-number":["R24 AG073199"]}],"id":[{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"publisher"}]},{"name":"National Institute on Aging","award":["R01 AG087957"],"award-info":[{"award-number":["R01 AG087957"]}],"id":[{"id":"https:\/\/ror.org\/049v75w11","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"accepted":{"date-parts":[[2026,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                <jats:p>\n                  Age-related decline in skeletal muscle function, often manifesting as slower gait speed, contributes significantly to frailty and loss of independence. To uncover the molecular basis of this decline, we investigated coordinated changes across the transcriptome, miRNome, and methylome in vastus lateralis muscle from female vervet monkeys (\n                  <jats:italic>Chlorocebus aethiops sabaeus<\/jats:italic>\n                  ), contrasting middle-aged animals with normal gait speed versus older animals with declining gait speed. Through multi-omics integration, we identified a molecular signature that sharply distinguishes these two groups. This signature involves key regulatory molecules, including miR-181a-5p, miR-320b and miR-425 control of\n                  <jats:italic>SYNCRIP<\/jats:italic>\n                  , miR-193b-3p regulation of\n                  <jats:italic>MCL1<\/jats:italic>\n                  and\n                  <jats:italic>PLAU<\/jats:italic>\n                  , as well as a multi-omic regulatory network governing\n                  <jats:italic>ZNF274<\/jats:italic>\n                  and\n                  <jats:italic>ANP32E<\/jats:italic>\n                  gene expression, and altered levels of mRNAs such as\n                  <jats:italic>VEZT<\/jats:italic>\n                  . Functional annotation implicates miRNA signatures with inverse expression of their mRNA targets operating within systems of skeletal muscle biology, metabolism, and potential immune-inflammation mechanisms. Collectively, these findings reveal an interconnected network of epigenetic, post-transcriptional, and transcriptional alterations associated with impaired muscle function during aging. This molecular signature provides novel insights into potential mechanisms driving sarcopenia and highlights specific molecules as potential targets for interventions aimed at preserving muscle health.\n                <\/jats:p>\n                <jats:p>\n                  <jats:fig id=\"ufig1\" position=\"float\" fig-type=\"figure\" orientation=\"portrait\">\n                    <jats:graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"754162v1_ufig1\" position=\"float\" orientation=\"portrait\"\/>\n                  <\/jats:fig>\n                <\/jats:p>","DOI":"10.64898\/2026.09.25.754162","type":"posted-content","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T17:30:24Z","timestamp":1790875824000},"source":"Crossref","is-referenced-by-count":0,"title":["Integrative Multi-Omics Analysis Reveals Molecular Signatures of Age-Related Decline in Vervet Leg Skeletal Muscle"],"prefix":"10.64898","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-1356-9614","authenticated-orcid":false,"given":"Maitree","family":"Patel","sequence":"first","affiliation":[{"name":"Section of Molecular Medicine, Department of Internal Medicine, Wake Forest University School of Medicine, Winston Salem, NC"},{"name":"Center for Precision Medicine, Wake Forest University School of Medicine, Winston-Salem, NC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleanor V.","family":"Rudasi","sequence":"additional","affiliation":[{"name":"Section of Molecular Medicine, Department of Internal Medicine, Wake Forest University School of Medicine, Winston Salem, NC"},{"name":"Center for Precision Medicine, Wake Forest University School of Medicine, Winston-Salem, 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