{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T08:11:49Z","timestamp":1778227909323,"version":"3.51.4"},"reference-count":0,"publisher":"Caleo","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Int Adv Otol"],"abstract":"<jats:p>BACKGROUND: Vestibular schwannoma (VS) is a common benign intracranial tumor that often leads to progressive hearing loss (HL). Long non-coding RNA (lncRNA) plays an important regulatory role in HL. This study aims to explore the clinical significance and potential regulatory mechanisms of lncRNA PAXBP1-AS1.\r\nMETHODS: The PAXBP1-AS1 was screened through the GSE174389 dataset. Clinical data and serum were collected from 36 patients with VS-related non-HL and 47 patients with VS-related HL. The level of PAXBP1-AS1 in serum was determined by real-time quantitative polymerase chain reaction (RT-qPCR), and the receiver-operating characteristic (ROC) curve was used to assess its clinical significance. In vitro experiments, the expressions of PAXBP1-AS1, miR-124-3p, and tumor necrosis factor\u2013\u03b1 (TNF-\u03b1) were detected by RT-qPCR. Cell viability and apoptosis were detected by Cell Counting kit-8 (CCK-8) and flow cytometry. The regulatory mechanism was speculated by target prediction.\r\nRESULTS: LncRNA PAXBP1-AS1 was downregulated in the GSE174389 dataset. PAXBP1-AS1 was expressed at low levels in VS patients with HL. The 8ROC analysis demonstrated that PAXBP1-AS1 distinguished between the HL and non-HL groups in patients with VS. Overexpression of PAXBP1-AS1 could inhibit cell activity, promote cell apoptosis, and suppress the expression of TNF-\u03b1, while silencing PAXBP1-AS1 could enhance cell activity, inhibit cell apoptosis, and promote the expression of TNF-\u03b1. miR-124-3p can target and negatively regulate PAXBP1-AS1. The miR-124-3p mimic can partially reverse the effect of overexpressing PAXBP1-AS1.\r\nCONCLUSION: PAXBP1-AS1, as a diagnostic biomarker in VS-related HL, can affect HEI-193 cell viability, apoptosis, and inflammatory level by targeting and negatively regulating the miR-124-3p.<\/jats:p>","DOI":"10.65717\/iao.2026.252314","type":"journal-article","created":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:32:14Z","timestamp":1778225534000},"page":"1-7","source":"Crossref","is-referenced-by-count":0,"title":["Long Non-Coding RNA PAXBP1-AS1 Is Associated with Hearing Loss in Vestibular Schwannoma via Targeting miR-124-3p"],"prefix":"10.65717","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6807-6933","authenticated-orcid":false,"given":"Rui","family":"Da","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2653-4470","authenticated-orcid":false,"given":"Nannan","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-6359-6904","authenticated-orcid":false,"given":"Peng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-2180-0595","authenticated-orcid":false,"given":"Yang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2287-1662","authenticated-orcid":false,"given":"Rui","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"55248","published-online":{"date-parts":[[2026,5,6]]},"container-title":["The Journal of International Advanced Otology"],"original-title":[],"link":[{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/download\/1975\/1962","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/download\/1975\/1962","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T07:32:17Z","timestamp":1778225537000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.advancedotology.org\/index.php\/pub\/article\/view\/1975"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,6]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,4,29]]}},"URL":"https:\/\/doi.org\/10.65717\/iao.2026.252314","relation":{},"ISSN":["2148-3817"],"issn-type":[{"value":"2148-3817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,6]]}}}