{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T18:04:38Z","timestamp":1776708278690,"version":"3.51.2"},"reference-count":39,"publisher":"The Microbiological Society of Korea","issue":"4","license":[{"start":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T00:00:00Z","timestamp":1745884800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Department of Microbiology and Veterinary Public Health of Chattogram Veterinary"},{"name":"Animal Sciences University"},{"name":"Lundbeck Foundation","award":["R288-2018-1123"],"award-info":[{"award-number":["R288-2018-1123"]}]}],"content-domain":{"domain":["jmicrobiol.or.kr"],"crossmark-restriction":true},"short-container-title":["Journal of Microbiology"],"published-print":{"date-parts":[[2025,4,30]]},"abstract":"<jats:p>Antimicrobial resistance (AMR) poses a serious threat to public health, with the emergence of extended-spectrum beta-lactamases (ESBLs) in Enterobacteriaceae, particularly Escherichia coli, raising significant concerns. This study aims to elucidate the drivers of antimicrobial resistance, and the global spread of cefotaxime-resistant E. coli (CREC) strains. Whole-genome sequencing (WGS) was performed to explore genome-level characteristics, and phylogenetic analysis was conducted to compare twenty CREC strains from this study, which were isolated from broiler chicken farms in Bangladesh, with a global collection (n = 456) of CREC strains from multiple countries and hosts. The MIC analysis showed over 70% of strains isolated from broiler chickens exhibiting MIC values \u2265 256 mg\/L for cefotaxime. Notably, 85% of the studied farms (17\/20) tested positive for CREC by the end of the production cycle, with CREC counts increasing from 0.83\u2009\u00b1\u20091.75 log10 CFU\/g feces on day 1 to 5.24\u2009\u00b1\u20090.72 log10 CFU\/g feces by day 28. WGS revealed the presence of multiple resistance genes, including blaCTX-M, which was found in 30% of the strains. Phylogenetic comparison showed that the Bangladeshi strains were closely related to strains from diverse geographical regions and host species. This study provides a comprehensive understanding of the molecular epidemiology of CREC. The close phylogenetic relationships between Bangladeshi and global strains demonstrate the widespread presence of cefotaxime-resistant bacteria and emphasize the importance of monitoring AMR in food-producing animals to mitigate the spread of resistant strains.<\/jats:p>","DOI":"10.71150\/jm.2412009","type":"journal-article","created":{"date-parts":[[2025,4,29]],"date-time":"2025-04-29T04:18:37Z","timestamp":1745900317000},"page":"e2412009","update-policy":"https:\/\/doi.org\/10.71150\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Whole-genome characterization and global phylogenetic comparison of cefotaxime-resistant Escherichia coli isolated from broiler 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