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The composite was successfully synthesized and characterized, revealing distinct physicochemical properties, uniform dispersion, and confirmed coordination between CMCh and Ag\u207a. In vitro evaluations, including minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC), growth curve, and plate spotting assays, demonstrated that CMCh-Ag\u207a exhibited significantly superior antifungal efficacy against multiple A. fumigatus strains (including azole-resistant isolates) compared to CMCh or Ag\u207a alone. In vivo experiments using a Galleria mellonella infection model confirmed the enhanced therapeutic effect and biocompatibility of CMCh-Ag\u207a. Investigations into the mechanism-related phenotypes revealed that CMCh-Ag\u207a significantly removed fungal biofilm and was associated with a substantial accumulation of intracellular reactive oxygen species (ROS), correlating with fungal cell death. This research highlights the preliminary potential of CMCh Ag\u207a as a candidate strategy to combat drug-resistant A. fumigatus infections, warranting further investigation in mammalian models to assess its clinical translational prospects.<\/jats:p>","DOI":"10.71150\/jm.2512001","type":"journal-article","created":{"date-parts":[[2026,4,6]],"date-time":"2026-04-06T05:51:27Z","timestamp":1775454687000},"page":"e2512001","update-policy":"https:\/\/doi.org\/10.71150\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobial effects and mechanism of action of carboxymethyl chitosan-loaded silver ion complexes against drug-resistant Aspergillus 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