{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T18:16:14Z","timestamp":1788286574579,"version":"build-2803163510"},"reference-count":28,"publisher":"Association of Biomolecular Resource Facilities","issue":"3","license":[{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R42GM113666"],"award-info":[{"award-number":["R42GM113666"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Severe allergic reactions can occur when biotherapeutics containing the carbohydrate antigen galactose-\u03b1-1,3-galactose (\u03b1-Gal) are administered. The detection of \u03b1-Gal-containing N-glycans is a challenging task due to the presence of non-\u03b1-Gal-containing isomers. This study evaluates the ability of different analytical approaches to detect an \u03b1-Gal glycan in the presence of a non-\u03b1-Gal isomer. Cetuximab is known to contain N-glycans bearing the \u03b1-Gal epitope and was chosen for the study. N-glycans released from Cetuximab were analyzed using the HILIC-MS\/MS system. HILIC-MS\/MS successfully separated and identified \u03b1-Gal species when the Galactose number exceeded the antenna number. However, this system could not identify \u03b1-Gal species when the Galactose number equals the antenna number. A High-Resolution Ion Mobility Spectrometer (IMS) was also evaluated and found to resolve isomeric \u03b1-gal\/non-\u03b1-gal isomeric glycans. The study demonstrates the utility of the IMS-MS system by detecting immunogenic glycans in the presence of their non-\u03b1-Gal isomers. To our knowledge, this is the first time this specific immunogenic pair of glycan isomers has been resolved in a biotherapeutic, which was enabled by a high-resolution IMS separation.<\/jats:p>","DOI":"10.7171\/001c.165893","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T17:44:27Z","timestamp":1788284667000},"source":"Crossref","is-referenced-by-count":0,"title":["High Resolution Ion Mobility Enables the Separation of \u03b1-Galactose Containing Glycans from Their Non-\u03b1-Galactose Isomers"],"prefix":"10.7171","volume":"37","author":[{"given":"Kimmy","family":"Park","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02bjhwk41","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Georgia","department":["Complex Carbohydrate Research Center"]},{"id":[{"id":"https:\/\/ror.org\/02bjhwk41","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Georgia","department":["Department of Chemistry"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4111-8326","authenticated-orcid":true,"given":"Ron","family":"Orlando","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02bjhwk41","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Georgia","department":["Complex Carbohydrate Research Center"]},{"name":"GlycoScientific, Athens, GA, 30602"},{"id":[{"id":"https:\/\/ror.org\/02bjhwk41","id-type":"ROR","asserted-by":"publisher"}],"name":"University of Georgia","department":["Department of Biochemistry and Molecular Biology"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4185","published-online":{"date-parts":[[2026,9,1]]},"reference":[{"issue":"17","key":"ref1","doi-asserted-by":"publisher","first-page":"7867","DOI":"10.1021\/acs.chemrev.7b00669","article-title":"Capillary electrophoresis separations of glycans","volume":"118","author":"G. 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