{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:16:25Z","timestamp":1788243385210,"version":"build-2803163510"},"reference-count":24,"publisher":"Korean Cleft Palate-Craniofacial Association","issue":"4","license":[{"start":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T00:00:00Z","timestamp":1787184000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["e-acfs.org"],"crossmark-restriction":true},"short-container-title":["Arch Craniofac Surg"],"published-print":{"date-parts":[[2026,8,20]]},"abstract":"<jats:p>Background: Jaw bone regeneration remains a clinical challenge in maxillofacial surgery. Bioactive ceramics and neural crest-derived multipotent mesenchymal stem cells (NC-MSCs) show regenerative potential, but their combined effect in repairing jaw defects has not been fully investigated. To our knowledge, no previous studies have evaluated germanium-doped biphasic calcium phosphate ceramics combined with NC-MSCs from vibrissae in a critical-size mandibular defect model. This study aimed to evaluate biphasic calcium phosphate ceramics doped with 1% germanium (BCPdGe 1%) alone versus BCPdGe 1% combined with NC-MSCs on rabbit mandibular defect regeneration and NC-MSC viability from rabbit vibrissae.Methods: NC-MSC properties on modified ceramics were assessed in vitro using microscopic, cytochemical, and colorimetric analyses. Nine ceramic samples with different Ge and SiO2 concentrations and NC-MSCs from rabbit vibrissae were tested. The BCPdGe 1% group without stem cells was previously evaluated in the same rabbit mandibular defect model and used as a reference control following ethical 3R principles. In vivo, 30 rabbits with critical-size mandibular defects (7 mm) were assigned to BCPdGe 1% (n= 15) or BCPdGe 1%+NC-MSCs (n= 15). Bone regeneration was analyzed histologically, and data were processed in EZR (p&lt; 0.05).Results: NC-MSCs showed the highest viability with samples no. 3 (pure hydroxyapatite doped with 1.5% germanium), no. 5 (BCPdGe 1%), and no. 7 (biphasic calcium phosphate ceramics doped with SiO2). In vivo, BCPdGe 1% alone achieved significantly better bone regeneration than BCPdGe 1% combined with NC-MSCs.Conclusion: Implanted NC-MSCs are highly sensitive to local biological conditions and mechanical stress, highlighting the need for further research on stem cell sources and cultivation methods.<\/jats:p>","DOI":"10.7181\/acfs.2026.0026","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T05:41:18Z","timestamp":1787895678000},"page":"180-188","update-policy":"https:\/\/doi.org\/10.7181\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Germanium-doped calcium phosphate ceramics in combination with mesenchymal stem cells derived from the neural crest, obtained from rabbit vibrissae: an experimental model of rabbit jaw regeneration"],"prefix":"10.7181","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-4608-4893","authenticated-orcid":true,"given":"Anna","family":"Zhmurko","sequence":"first","affiliation":[{"name":"Department of Maxillofacial Surgery, Bogomolets National Medical University, Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7489-7170","authenticated-orcid":true,"given":"Roman","family":"Palyvoda","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery, Bogomolets National Medical University, Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8806-0280","authenticated-orcid":true,"given":"Natalia","family":"Ulyanchich","sequence":"additional","affiliation":[{"name":"I.M. Frantsevich Institute for Problems of Materials Science, National Academy of Sciences of Ukraine, Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5475-5642","authenticated-orcid":true,"given":"Valeryi","family":"Chemerovskyi","sequence":"additional","affiliation":[{"name":"Department of Surgery and Diseases of Small Domestic Animals, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9856-9793","authenticated-orcid":true,"given":"Tetyana","family":"Todosiuk","sequence":"additional","affiliation":[{"name":"Department of Surgery and Diseases of Small Domestic Animals, Bila Tserkva National Agrarian University, Bila Tserkva, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5834-2834","authenticated-orcid":true,"given":"Alona","family":"Zlatska","sequence":"additional","affiliation":[{"name":"Institute of Genetic and Regenerative Medicine, M. 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Strazhesko National Scientific Center of Cardiology, Clinical and Regenerative Medicine, National Academy of Medical Sciences of Ukraine, Kyiv, Ukraine"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4182","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26103007","type":"journal-article"},{"key":"ref2","first-page":"20417314","type":"journal-article","volume-title":"Bone substitutes: a review of their characteristics, clinical use, and perspectives for large bone defects management","author":"Fernandez de Grado","year":"2018"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.3390\/jcm14061838","type":"journal-article"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2011.06.014","type":"journal-article"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2017.05.007","type":"journal-article"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.36740\/merkur202502113","type":"journal-article"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22094412","type":"journal-article"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1444986","type":"journal-article"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.3390\/jfb14050286","type":"journal-article"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202202766","type":"journal-article"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-023-03611-1","type":"journal-article"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.3390\/cimb47040287","type":"journal-article"},{"key":"ref13","first-page":"e247","type":"journal-article","volume-title":"Efficacy of stem cells in bone rehabilitation in patients with alveolar bone atrophy: a systematic review","author":"Perez-Leal","year":"2023"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3390\/ma16114117","type":"journal-article"},{"key":"ref15","first-page":"1609082","type":"journal-article","volume-title":"Hypoxia-induced metabolic reprogramming in mesenchymal stem cells: unlocking the regenerative potential of secreted factors","author":"Jaraba-Alvarez","year":"2025"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1976","type":"journal-article"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2014.164","type":"journal-article"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1038\/s41368-019-0060-3","type":"journal-article"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.23213","type":"journal-article"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1093\/asj\/sjaa154","type":"journal-article"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19071897","type":"journal-article"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1007\/s40778-016-0053-5","type":"journal-article"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2849879","type":"journal-article"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-015-2116-9","type":"journal-article"}],"container-title":["Archives of Craniofacial Surgery"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/e-acfs.org\/upload\/pdf\/acfs-2026-0026.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-acfs.org\/journal\/view.php?doi=10.7181\/acfs.2026.0026","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/e-acfs.org\/upload\/pdf\/acfs-2026-0026.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:01:42Z","timestamp":1788242502000},"score":1,"resource":{"primary":{"URL":"http:\/\/e-acfs.org\/journal\/view.php?doi=10.7181\/acfs.2026.0026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,20]]},"references-count":24,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,8]]}},"URL":"https:\/\/doi.org\/10.7181\/acfs.2026.0026","relation":{},"ISSN":["2287-1152","2287-5603"],"issn-type":[{"value":"2287-1152","type":"print"},{"value":"2287-5603","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,20]]},"assertion":[{"value":"2026-01-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":2,"name":"published","label":"Published online","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-20","order":3,"name":"published","label":"Print publication","group":{"name":"publication_history","label":"Publication History"}}]}}