{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:21:55Z","timestamp":1783506115991,"version":"3.55.0"},"reference-count":8,"publisher":"MDPI AG","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,3,1]]},"abstract":"<jats:p>Traditional methods of diagnosing onychomycosis, such as microscopy, histologic staining, and cultures, may not provide the clinician with documentation before initiating antifungal drug therapy. DNA technology now supplies the tools for increased sensitivity, speed, and accuracy in the diagnostic arena by allowing for the amplification, qualification, and quantitation of DNA. These techniques, already being used to identify many infectious agents, may soon be commonly applied to onychomycosis. This report reviews some of the DNA-based techniques that are currently being used to identify dermatophytes and their possible diagnostic use. (J Am Podiatr Med Assoc 97(2): 134\u2013144, 2007)<\/jats:p>","DOI":"10.7547\/0970134","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T10:14:52Z","timestamp":1396347292000},"page":"134-144","source":"Crossref","is-referenced-by-count":11,"title":["Molecular Biology Techniques for Identifying Dermatophytes and Their Possible Use in Diagnosing Onychomycosis in Human Toenail"],"prefix":"10.3390","volume":"97","author":[{"given":"Judith M.","family":"Binstock","sequence":"first","affiliation":[{"name":"Division of Pre-Clinical Sciences, New York College of Podiatric Medicine, 1800 Park Ave, New York, NY 10035."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","reference":[{"key":"ref951","first-page":"315","article-title":"Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for in PCR Protocols ed by MA Academic London","author":"White","year":"1990"},{"key":"ref111","article-title":"National Web site Available at http diabetes niddk nih gov dm pubs statistics index htm Accessed","volume":"12","author":"Clearinghouse","year":"2007","journal-title":"Diabetes Information"},{"key":"ref1451","article-title":"Human Project information DNA forensics Available at http www ornl gov sci techresources Human Genome elsi forensics shtml Accessed","author":"February","year":"2007","journal-title":"Genome"},{"key":"ref2211","article-title":"US and Drug Administration Talk Paper Issues Health Advisory Regarding the Safety of Sporanox Products and Lamisil Tablets to Treat Fungal Infections US and Drug Administration Rockville MD","author":"May","year":"2001","journal-title":"Food Food"},{"key":"ref2071","first-page":"315","article-title":"Amplification and Direct Sequencing of Fungal Ribosomal RNA Genes for in PCR Protocols ed by MA Academic London","author":"White","year":"1990"},{"key":"ref1231","article-title":"National Web site Available at http diabetes niddk nih gov dm pubs statistics index htm Accessed","volume":"12","author":"Clearinghouse","year":"2007","journal-title":"Diabetes Information"},{"key":"ref331","article-title":"Human Project information DNA forensics Available at http www ornl gov sci techresources Human Genome elsi forensics shtml Accessed","author":"February","year":"2007","journal-title":"Genome"},{"key":"ref1091","article-title":"US and Drug Administration Talk Paper Issues Health Advisory Regarding the Safety of Sporanox Products and Lamisil Tablets to Treat Fungal Infections US and Drug Administration Rockville MD","author":"May","year":"2001","journal-title":"Food Food"}],"container-title":["Journal of the American Podiatric Medical Association"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/meridian.allenpress.com\/japma\/article-pdf\/97\/2\/134\/1418629\/0970134.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/meridian.allenpress.com\/japma\/article-pdf\/97\/2\/134\/1418629\/0970134.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:11:04Z","timestamp":1783505464000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1930-8264\/97\/2\/134"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3,1]]},"references-count":8,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2007,3,1]]}},"URL":"https:\/\/doi.org\/10.7547\/0970134","relation":{},"ISSN":["8750-7315","1930-8264"],"issn-type":[{"value":"8750-7315","type":"print"},{"value":"1930-8264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3,1]]}}}