{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T11:14:08Z","timestamp":1783509248589,"version":"3.55.0"},"reference-count":27,"publisher":"MDPI AG","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec id=\"st1\">\n                    <jats:title>Background:<\/jats:title>\n                    <jats:p>In vitro biomechanical testing of the human foot often involves the use of fresh frozen cadaveric specimens to investigate interventions that would be detrimental to human subjects. The Thiel method is an alternative embalming technique that maintains soft-tissue consistency similar to that of living tissue. However, its suitability for biomechanical testing is unknown. Thus, the aim of this study was to determine whether Thiel-embalmed foot specimens exhibit kinematic and kinetic biomechanical properties similar to those of fresh frozen specimens.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"st2\">\n                    <jats:title>Methods:<\/jats:title>\n                    <jats:p>An observational study design was conducted at a university biomechanics laboratory. Three cadavers had both limbs amputated, with one being fresh frozen and the other preserved by Thiel's embalming. Each foot was tested while undergoing plantarflexion and dorsiflexion in three states: unloaded and under loads of 10 and 20 kg. Their segment kinematics and foot pressure mapping were assessed simultaneously.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"st3\">\n                    <jats:title>Results:<\/jats:title>\n                    <jats:p>No statistically significant differences were detected between fresh frozen and Thiel-embalmed sample pairs regarding kinematics and kinetics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec id=\"st4\">\n                    <jats:title>Conclusions:<\/jats:title>\n                    <jats:p>These findings highlight similar kinematic and kinetic properties between fresh frozen and Thiel-embalmed foot specimens, thus possibly enabling these specimens to be interchanged due to the latter specimens' advantage of delayed decomposition. This can open innovative opportunities for the use of these specimens in applications related to the investigation of dynamic foot function in research and education.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.7547\/16-130","type":"journal-article","created":{"date-parts":[[2019,5,28]],"date-time":"2019-05-28T10:52:34Z","timestamp":1559040754000},"page":"113-121","source":"Crossref","is-referenced-by-count":3,"title":["Kinematic and Kinetic Comparison of Fresh Frozen and Thiel-Embalmed Human Feet for Suitability for Biomechanical Educational and Research Settings"],"prefix":"10.3390","volume":"109","author":[{"given":"Alfred","family":"Gatt","sequence":"first","affiliation":[{"name":"Department of Podiatry, Faculty of Health Sciences, University of Malta, Msida, Malta."},{"name":"Faculty of Health, Staffordshire University, Stoke on Trent, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Schembri-Wismayer","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine and Surgery, University of Malta, Msida, Malta."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nachiappan","family":"Chockalingam","sequence":"additional","affiliation":[{"name":"Department of Podiatry, Faculty of Health Sciences, University of Malta, Msida, Malta."},{"name":"Faculty of Health, Staffordshire University, Stoke on Trent, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Formosa","sequence":"additional","affiliation":[{"name":"Department of Podiatry, Faculty of Health Sciences, University of Malta, Msida, Malta."},{"name":"Faculty of Health, Staffordshire University, Stoke on Trent, United Kingdom."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","reference":[{"key":"i8750-7315-109-2-113-b01","series-title":"Bone","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1016\/j.bone.2010.08.012","article-title":"Effects of three different preservation methods on the mechanical properties of human and bovine cortical bone","volume":"47","author":"Unger \n              S,","year":"2010","unstructured":"Unger \n              S, \n            \n            \n              Blauth \n              M, \n            \n            \n              Schmoelz \n              W: \n            \n          \n          Effects of three different preservation methods on the mechanical properties of human and bovine cortical bone. Bone47: 1048, 2010.2073609410.1016\/j.bone.2010.08.012"},{"key":"i8750-7315-109-2-113-b02","series-title":"J Foot Ankle Res","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1186\/1757-1146-2-18","article-title":"Lessons from dynamic cadaver and invasive bone pin studies: do we know how the foot really moves during gait?","volume":"2","author":"Nester \n              CJ:","year":"2009","unstructured":"Nester \n              CJ: \n            \n          \n          Lessons from dynamic cadaver and invasive bone pin studies: do we know how the foot really moves during gait?\n          J Foot Ankle Res\n          2: 18, 2009.10.1186\/1757-1146-2-1819473480"},{"key":"i8750-7315-109-2-113-b03","series-title":"Clin Biomech (Bristol, Avon)","doi-asserted-by":"publisher","first-page":"1294","DOI":"10.1016\/j.clinbiomech.2008.07.007","article-title":"The effects of embalming using a 4% formalin solution on the compressive mechanical properties of human cortical bone","volume":"23","author":"Ohman \n              C,","year":"2008","unstructured":"Ohman \n              C, \n            \n            \n              Dall'Ara \n              E, \n            \n            \n              Baleani \n              M, \n            \n            et al: \n          The effects of embalming using a 4% formalin solution on the compressive mechanical properties of human cortical bone. Clin Biomech (Bristol, Avon)23: 1294, 2008.10.1016\/j.clinbiomech.2008.07.007"},{"key":"i8750-7315-109-2-113-b04","series-title":"Biomaterials","doi-asserted-by":"publisher","first-page":"1267","DOI":"10.1016\/0142-9612(95)98135-2","article-title":"Effect of formaldehyde fixation on some mechanical properties of bovine bone","volume":"16","author":"Currey \n              JD,","year":"1995","unstructured":"Currey \n              JD, \n            \n            \n              Brear \n              K, \n            \n            \n              Zioupos \n              P, \n            \n            et al: \n          Effect of formaldehyde fixation on some mechanical properties of bovine bone. Biomaterials16: 1267, 1995.10.1016\/0142-9612(95)98135-28589198"},{"key":"i8750-7315-109-2-113-b05","series-title":"Clin Anat","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1002\/ca.22585","article-title":"Human preserevation techniques in anatomy: a 21st century medical education perspective","volume":"28","author":"Balta \n              JY,","year":"2015","unstructured":"Balta \n              JY, \n            \n            \n              Cronin \n              M, \n            \n            \n              Cryan \n              JF, \n            \n            et al: \n          Human preserevation techniques in anatomy: a 21st century medical education perspective. Clin Anat28: 725, 2015.10.1002\/ca.2258526118424"},{"key":"i8750-7315-109-2-113-b06","series-title":"Ann Anat","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1016\/j.aanat.2011.03.007","article-title":"Suitability of Thiel embalmed tendons for biomechanical investigation","volume":"193","author":"Fessel \n              G,","year":"2011","unstructured":"Fessel \n              G, \n            \n            \n              Frey \n              K, \n            \n            \n              Schweizer \n              A, \n            \n            et al: \n          Suitability of Thiel embalmed tendons for biomechanical investigation. Ann Anat193: 237, 2011.2151144710.1016\/j.aanat.2011.03.007"},{"key":"i8750-7315-109-2-113-b07","series-title":"Ann Anat","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S0940-9602(11)80346-8","article-title":"The preservation of the whole corpse with natural color","volume":"174","author":"Thiel \n              W:","year":"1992","unstructured":"Thiel \n              W: \n            \n          \n          The preservation of the whole corpse with natural color. Ann Anat174: 185, 1992.10.1016\/S0940-9602(11)80346-81503236"},{"key":"i8750-7315-109-2-113-b08","series-title":"Surgeon","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/j.surge.2010.09.001","article-title":"A comparison of Thiel and formalin embalmed cadavers for thyroid surgery training","volume":"9","author":"Eisma \n              R,","year":"2011","unstructured":"Eisma \n              R, \n            \n            \n              Mahendran \n              S, \n            \n            \n              Majumdar \n              S, \n            \n            et al: \n          A comparison of Thiel and formalin embalmed cadavers for thyroid surgery training. Surgeon9: 142, 2011.2155051910.1016\/j.surge.2010.09.001"},{"key":"i8750-7315-109-2-113-b09","series-title":"Surg Radiol Anat","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1007\/s00276-010-0703-8","article-title":"Flexibility of Thiel's embalmed cadavers: the explanation is probably in the muscles","volume":"33","author":"Benkhadra \n              M,","year":"2011","unstructured":"Benkhadra \n              M, \n            \n            \n              Bouchot \n              A, \n            \n            \n              Gerard \n              J, \n            \n            et al: \n          Flexibility of Thiel's embalmed cadavers: the explanation is probably in the muscles. Surg Radiol Anat33: 365, 2011.10.1007\/s00276-010-0703-820632174"},{"key":"i8750-7315-109-2-113-b10","series-title":"Surg Endosc","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1007\/s00464-007-9502-7","article-title":"Laparoscopic training on Thiel human cadavers: a model to teach advanced laparoscopic procedures","volume":"22","author":"Giger \n              U,","year":"2008","unstructured":"Giger \n              U, \n            \n            \n              Fresard \n              I, \n            \n            \n              Hafliger \n              A, \n            \n            et al: \n          Laparoscopic training on Thiel human cadavers: a model to teach advanced laparoscopic procedures. Surg Endosc22: 901, 2008.10.1007\/s00464-007-9502-7"},{"key":"i8750-7315-109-2-113-b11","series-title":"Clin Implant Dent Relat Res","doi-asserted-by":"publisher","first-page":"121","DOI":"10.1111\/j.1708-8208.2009.00230.x","article-title":"Thiel embalming technique: a valuable method for teaching oral surgery and implantology","volume":"14","author":"Holzle \n              F,","year":"2012","unstructured":"Holzle \n              F, \n            \n            \n              Franz \n              EP, \n            \n            \n              Lehmbrock \n              J, \n            \n            et al: \n          Thiel embalming technique: a valuable method for teaching oral surgery and implantology. Clin Implant Dent Relat Res14: 121, 2012.1967395510.1111\/j.1708-8208.2009.00230.x"},{"key":"i8750-7315-109-2-113-b12","series-title":"Mund Kiefer Gesichtschir","doi-asserted-by":"publisher","first-page":"141","DOI":"10.1007\/PL00010796","article-title":"Surgical procedures in mouth, jaw and facial surgery in Thiel embalmed body donors","volume":"5","author":"Peuker \n              ET,","year":"2001","unstructured":"Peuker \n              ET, \n            \n            \n              Werkmeister \n              R, \n            \n            \n              Pera \n              F, \n            \n            et al: \n          Surgical procedures in mouth, jaw and facial surgery in Thiel embalmed body donors. Mund Kiefer Gesichtschir5: 141, 2001.10.1007\/PL0001079611372181"},{"key":"i8750-7315-109-2-113-b13","series-title":"Clin Oral Implants Res","doi-asserted-by":"publisher","first-page":"521","DOI":"10.1111\/j.1600-0501.2008.01682.x","article-title":"Fresh-frozen vs. embalmed bone: is it possible to use formalin-fixed human bone for biomechanical experiments on implants?","volume":"20","author":"Comert \n              A,","year":"2009","unstructured":"Comert \n              A, \n            \n            \n              Kokat \n              AM, \n            \n            \n              Akkocaoglu \n              M, \n            \n            et al: \n          Fresh-frozen vs. embalmed bone: is it possible to use formalin-fixed human bone for biomechanical experiments on implants?\n          Clin Oral Implants Res\n          20: 521, 2009.10.1111\/j.1600-0501.2008.01682.x19522977"},{"key":"i8750-7315-109-2-113-b14","series-title":"J Biomech","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1016\/j.jbiomech.2006.09.008","article-title":"In vitro study of foot kinematics using a dynamic walking cadaver model","volume":"40","author":"Nester \n              CJ,","year":"2007","unstructured":"Nester \n              CJ, \n            \n            \n              Liu \n              AM, \n            \n            \n              Ward \n              E, \n            \n            et al: \n          In vitro study of foot kinematics using a dynamic walking cadaver model. J Biomech40: 1927, 2007.10.1016\/j.jbiomech.2006.09.00817081548"},{"key":"i8750-7315-109-2-113-b15","series-title":"Clin Biomech (Bristol, Avon)","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1016\/S0268-0033(98)00003-5","article-title":"A dynamic cadaver model of the stance phase of gait: performance characteristics and kinetic validation","volume":"13","author":"Sharkey \n              NA,","year":"1998","unstructured":"Sharkey \n              NA, \n            \n            \n              Hamel \n              AJ: \n            \n          \n          A dynamic cadaver model of the stance phase of gait: performance characteristics and kinetic validation. Clin Biomech (Bristol, Avon)13: 420, 1998.10.1016\/S0268-0033(98)00003-5"},{"key":"i8750-7315-109-2-113-b16","series-title":"Foot Ankle Int","doi-asserted-by":"publisher","first-page":"881","DOI":"10.1177\/107110070302401202","article-title":"Kinematic changes after fusion and total replacement of the ankle: part 1. Range of motion","volume":"24","author":"Valderrabano \n              V,","year":"2003","unstructured":"Valderrabano \n              V, \n            \n            \n              Hintermann \n              B, \n            \n            \n              Nigg \n              BM, \n            \n            et al: \n          Kinematic changes after fusion and total replacement of the ankle: part 1. Range of motion. Foot Ankle Int24: 881, 2003.1473334110.1177\/107110070302401202"},{"key":"i8750-7315-109-2-113-b17","series-title":"Gait Posture","doi-asserted-by":"publisher","first-page":"125","DOI":"10.1016\/S0966-6362(02)00063-2","article-title":"An in vitro study of individual ankle muscle actions on the center of pressure","volume":"17","author":"Kim \n              KJ,","year":"2003","unstructured":"Kim \n              KJ, \n            \n            \n              Uchiyama \n              E, \n            \n            \n              Kitaoka \n              HB, \n            \n            et al: \n          An in vitro study of individual ankle muscle actions on the center of pressure. Gait Posture17: 125, 2003.10.1016\/S0966-6362(02)00063-2"},{"key":"i8750-7315-109-2-113-b18","series-title":"Clin Biomech (Bristol, Avon)","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1016\/S0268-0033(98)00011-4","article-title":"Measurement of surface contact area of the ankle joint","volume":"13","author":"Kura \n              H,","year":"1998","unstructured":"Kura \n              H, \n            \n            \n              Kitaoka \n              HB, \n            \n            \n              Luo \n              ZP, \n            \n            et al: \n          Measurement of surface contact area of the ankle joint. Clin Biomech (Bristol, Avon)13: 365, 1998.10.1016\/S0268-0033(98)00011-4"},{"key":"i8750-7315-109-2-113-b19","series-title":"Osteoarthritis Cartilage","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/j.joca.2005.09.010","article-title":"Stance-phase aggregate contact stress and contact stress gradient changes resulting from articular surface stepoffs in human cadaveric ankles","volume":"14","author":"McKinley \n              TO,","year":"2006","unstructured":"McKinley \n              TO, \n            \n            \n              McKinley \n              T, \n            \n            \n              Rudert \n              MJ, \n            \n            et al: \n          Stance-phase aggregate contact stress and contact stress gradient changes resulting from articular surface stepoffs in human cadaveric ankles. Osteoarthritis Cartilage14: 131, 2006.1628973410.1016\/j.joca.2005.09.010"},{"key":"i8750-7315-109-2-113-b20","series-title":"Kurume Med J","doi-asserted-by":"publisher","first-page":"75","DOI":"10.2739\/kurumemedj.45.75","article-title":"Impact loading on the foot and ankle and its attenuation during level walking","volume":"45","author":"Miyazaki \n              K.","year":"1998","unstructured":"Miyazaki \n              K. \n            \n          \n          Impact loading on the foot and ankle and its attenuation during level walking. Kurume Med J45: 75, 1998.10.2739\/kurumemedj.45.759658755"},{"key":"i8750-7315-109-2-113-b21","series-title":"Foot Ankle Int","doi-asserted-by":"publisher","first-page":"44","DOI":"10.3113\/FAI.2009.0044","article-title":"Cadaveric simulation of a pes cavus foot","volume":"30","author":"Daines \n              SB,","year":"2009","unstructured":"Daines \n              SB, \n            \n            \n              Rohr \n              ES, \n            \n            \n              Pace \n              AP, \n            \n            et al: \n          Cadaveric simulation of a pes cavus foot. Foot Ankle Int30: 44, 2009.10.3113\/FAI.2009.004419176185"},{"key":"i8750-7315-109-2-113-b22","series-title":"Paper presented at: 45th Annual Meeting of the Orthopaedic Research Society, February 1-4","article-title":"Analysis of ankle and foot motion with a dynamic ankle simulator: a cadaver mode","author":"Kitaoka \n              HB,","year":"1999","unstructured":"Kitaoka \n              HB, \n            \n            \n              Luo \n              ZP, \n            \n            \n              Uchiyama \n              E, \n            \n            et al\n          \n          , eds:\n          Analysis of ankle and foot motion with a dynamic ankle simulator: a cadaver mode. Paper presented at: 45th Annual Meeting of the Orthopaedic Research Society, February 1-4, 1999, Anaheim, CA."},{"key":"i8750-7315-109-2-113-b23","series-title":"Arch Phys Med Rehabil","doi-asserted-by":"publisher","first-page":"876","DOI":"10.1053\/apmr.2002.32681","article-title":"Effect of foot orthoses on 3-dimensional kinematics of flatfoot: a cadaveric study","volume":"83","author":"Kitaoka \n              HB,","year":"2002","unstructured":"Kitaoka \n              HB, \n            \n            \n              Luo \n              ZP, \n            \n            \n              Kura \n              H, \n            \n            et al: \n          Effect of foot orthoses on 3-dimensional kinematics of flatfoot: a cadaveric study. Arch Phys Med Rehabil83: 876, 2002.1204867210.1053\/apmr.2002.32681"},{"key":"i8750-7315-109-2-113-b24","series-title":"J Biomech","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1016\/j.jbiomech.2006.09.008","article-title":"In vitro study of foot kinematics using a dynamic walking cadaver model","volume":"40","author":"Nester \n              CJ,","year":"2007","unstructured":"Nester \n              CJ, \n            \n            \n              Liu \n              AM, \n            \n            \n              Ward \n              E, \n            \n            et al. \n          In vitro study of foot kinematics using a dynamic walking cadaver model. J Biomech40: 1927, 2007.10.1016\/j.jbiomech.2006.09.00817081548"},{"key":"i8750-7315-109-2-113-b25","series-title":"J Biomech","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/0021-9290(89)90080-8","article-title":"An experimental setup for the measurement of forces on a human cadaveric foot during inversion","volume":"22","author":"Ottevanger \n              EJ,","year":"1989","unstructured":"Ottevanger \n              EJ, \n            \n            \n              Spoor \n              CW, \n            \n            \n              van Leeuwen \n              JL, \n            \n            et al: \n          An experimental setup for the measurement of forces on a human cadaveric foot during inversion. J Biomech22: 957, 1989.10.1016\/0021-9290(89)90080-82613730"},{"key":"i8750-7315-109-2-113-b26","series-title":"Clin Anat","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1002\/ca.22222","article-title":"From formalin to Thiel embalming: what changes? one anatomy department's experiences","volume":"26","author":"Eisma \n              R,","year":"2013","unstructured":"Eisma \n              R, \n            \n            \n              Lamb \n              C, \n            \n            \n              Soames \n              RW: \n            \n          \n          From formalin to Thiel embalming: what changes? one anatomy department's experiences. Clin Anat26: 564, 2013.2340838610.1002\/ca.22222"},{"key":"i8750-7315-109-2-113-b27","series-title":"Gait Posture","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1016\/j.gaitpost.2006.05.017","article-title":"Rear-foot, mid-foot and fore-foot motion during the stance phase of gait","volume":"25","author":"Leardini \n              A,","year":"2007","unstructured":"Leardini \n              A, \n            \n            \n              Benedetti \n              MG, \n            \n            \n              Berti \n              L, \n            \n            et al: \n          Rear-foot, mid-foot and fore-foot motion during the stance phase of gait. Gait Posture25: 453, 2007.10.1016\/j.gaitpost.2006.05.01716965916"}],"container-title":["Journal of the American Podiatric Medical Association"],"original-title":[],"link":[{"URL":"https:\/\/japmaonline.org\/view\/journals\/apms\/109\/2\/16-130.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/meridian.allenpress.com\/japma\/article-pdf\/109\/2\/113\/2457756\/16-130.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/japmaonline.org\/downloadpdf\/journals\/apms\/109\/2\/16-130.xml","content-type":"text\/html","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/japmaonline.org\/downloadpdf\/journals\/apms\/109\/2\/16-130.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T10:17:38Z","timestamp":1783505858000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1930-8264\/109\/2\/113"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3]]},"references-count":27,"journal-issue":{"issue":"2"},"URL":"https:\/\/doi.org\/10.7547\/16-130","relation":{},"ISSN":["8750-7315","1930-8264"],"issn-type":[{"value":"8750-7315","type":"print"},{"value":"1930-8264","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3]]}}}