{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T03:34:11Z","timestamp":1779161651769,"version":"3.51.4"},"reference-count":80,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000875","name":"Pew Charitable Trusts","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000875","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["1K99GM114397-01"],"award-info":[{"award-number":["1K99GM114397-01"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001033","name":"Jane Coffin Childs Memorial Fund for Medical Research","doi-asserted-by":"publisher","award":["JCCF Postdoctoral Fellowship"],"award-info":[{"award-number":["JCCF Postdoctoral Fellowship"]}],"id":[{"id":"10.13039\/100001033","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009220","name":"UC Berkeley College of Chemistry","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009220","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007247","name":"Adolph C. and Mary Sprague Miller Institute for Basic Research in Science, University of California Berkeley","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007247","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Recent evidence suggests that autophagy facilitates the unconventional secretion of the pro-inflammatory cytokine interleukin 1\u03b2 (IL-1\u03b2). Here, we reconstituted an autophagy-regulated secretion of mature IL-1\u03b2 (m-IL-1\u03b2) in non-macrophage cells. We found that cytoplasmic IL-1\u03b2 associates with the autophagosome and m-IL-1\u03b2 enters into the lumen of a vesicle intermediate but not into the cytoplasmic interior formed by engulfment of the autophagic membrane. In advance of secretion, m-IL-1\u03b2 appears to be translocated across a membrane in an event that may require m-IL-1\u03b2 to be unfolded or remain conformationally flexible and is dependent on two KFERQ-like motifs essential for the association of IL-1\u03b2 with HSP90. A vesicle, possibly a precursor of the phagophore, contains translocated m-IL-1\u03b2 and later turns into an autophagosome in which m-IL-1\u03b2 resides within the intermembrane space of the double-membrane structure. Completion of IL-1\u03b2 secretion requires Golgi reassembly and stacking proteins (GRASPs) and multi-vesicular body (MVB) formation.<\/jats:p>","DOI":"10.7554\/elife.11205","type":"journal-article","created":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T07:52:21Z","timestamp":1446450741000},"source":"Crossref","is-referenced-by-count":335,"title":["Translocation of interleukin-1\u03b2 into a vesicle intermediate in autophagy-mediated secretion"],"prefix":"10.7554","volume":"4","author":[{"given":"Min","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel J","family":"Kenny","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Berkeley, Berkeley, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7371-2039","authenticated-orcid":true,"given":"Liang","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2788-194X","authenticated-orcid":true,"given":"Ke","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of California, Berkeley, Berkeley, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8615-6409","authenticated-orcid":true,"given":"Randy","family":"Schekman","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, Howard Hughes Medical Institute, University of California, Berkeley, Berkeley, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4374","published-online":{"date-parts":[[2015,11,2]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1091\/mbc.10.5.1463","article-title":"The secretory route of the leaderless protein interleukin 1beta involves exocytosis of endolysosome-related vesicles","volume":"10","author":"Andrei","year":"1999","journal-title":"Molecular Biology of the Cell"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"9745","DOI":"10.1073\/pnas.0308558101","article-title":"From the cover: phospholipases c and A2 control lysosome-mediated IL-1 secretion: implications for inflammatory processes","volume":"101","author":"Andrei","year":"2004","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"1727","DOI":"10.1242\/jcs.01027","article-title":"Unconventional protein secretion: membrane translocation of FGF-2 does not require protein unfolding","volume":"117","author":"Backhaus","year":"2004","journal-title":"Journal of Cell Science"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"5747","DOI":"10.1128\/MCB.02070-07","article-title":"The chaperone-mediated autophagy receptor organizes in dynamic protein complexes at the lysosomal membrane","volume":"28","author":"Bandyopadhyay","year":"2008","journal-title":"Molecular and Cellular Biology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"122","DOI":"10.1038\/nrm732","article-title":"SECRETORY LYSOSOMES","volume":"3","author":"Blott","year":"2002","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"3029","DOI":"10.4049\/jimmunol.170.6.3029","article-title":"Ca2+ stores and Ca2+ entry differentially contribute to the release of IL-1 and IL-1 from murine macrophages","volume":"170","author":"Brough","year":"2003","journal-title":"The Journal of Immunology"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"979","DOI":"10.1007\/978-3-642-00302-8_1","article-title":"Biogenesis of a novel compartment for autophagosome-mediated unconventional protein secretion","volume":"195","author":"Bruns","year":"2011","journal-title":"The Journal of Cell Biology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"26","DOI":"10.1016\/S0952-7915(02)00017-1","article-title":"New insights into the mechanism of IL-1 maturationn","volume":"15","author":"Burns","year":"2003","journal-title":"Current Opinion in Immunology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1126\/science.1373520","article-title":"Molecular cloning of the interleukin-1 beta converting enzyme","volume":"256","author":"Cerretti","year":"1992","journal-title":"Science"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1016\/j.celrep.2014.06.028","article-title":"The neutrophil NLRC4 inflammasome selectively promotes IL-1 maturation without pyroptosis during acute salmonella challengee","volume":"8","author":"Chen","year":"2014","journal-title":"Cell Reports"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1126\/science.2799391","article-title":"A role for a 70-kilodalton heat shock protein in lysosomal degradation of intracellular proteins","volume":"246","author":"Chiang","year":"1989","journal-title":"Science"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"397","DOI":"10.1016\/j.tcb.2012.04.008","article-title":"Autophagy intersections with conventional and unconventional secretion in tissue development, remodeling and inflammation","volume":"22","author":"Deretic","year":"2012","journal-title":"Trends in Cell Biology"},{"key":"bib13","doi-asserted-by":"crossref","first-page":"6853","DOI":"10.1016\/S0021-9258(19)62694-3","article-title":"Regulation of catabolism of microinjected ribonuclease a. identification of residues 7-11 as the essential pentapeptide","volume":"261","author":"Dice","year":"1986","journal-title":"The Journal of Biological Chemistry"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1016\/j.tplants.2012.06.004","article-title":"Unconventional protein secretion","volume":"17","author":"Ding","year":"2012","journal-title":"Trends in Plant Science"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"4701","DOI":"10.1038\/emboj.2011.398","article-title":"Autophagy-based unconventional secretory pathway for extracellular delivery of IL-1","volume":"30","author":"Dupont","year":"2011","journal-title":"The EMBO Journal"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1091\/mbc.E07-12-1292","article-title":"Unconventional secretion of Acb1 is mediated by autophagosomes","volume":"188","author":"Duran","year":"2010","journal-title":"The Journal of Cell Biology"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1038\/322228a0","article-title":"Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria","volume":"322","author":"Eilers","year":"1986","journal-title":"Nature"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1038\/cr.2013.168","article-title":"The machinery of macroautophagy","volume":"24","author":"Feng","year":"2014","journal-title":"Cell Research"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"4651","DOI":"10.1091\/mbc.E08-03-0312","article-title":"An Atg4B mutant hampers the lipidation of LC3 paralogues and causes defects in autophagosome closure","volume":"19","author":"Fujita","year":"2008","journal-title":"Molecular Biology of the Cell"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"e00947","DOI":"10.7554\/eLife.00947.028","article-title":"The ER\u2013golgi intermediate compartment is a key membrane source for the LC3 lipidation step of autophagosome biogenesis","volume":"2","author":"Ge","year":"2013","journal-title":"eLife"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.ceb.2014.02.005","article-title":"The protein-vesicle network of autophagy","volume":"29","author":"Ge","year":"2014","journal-title":"Current Opinion in Cell Biology"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"e04135","DOI":"10.7554\/eLife.04135.018","article-title":"Phosphatidylinositol 3-kinase and COPII generate LC3 lipidation vesicles from the ER-golgi intermediate compartment","volume":"3","author":"Ge","year":"2014","journal-title":"eLife"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1016\/j.ceb.2011.04.005","article-title":"Unconventional secretion: a stress on GRASP","volume":"23","author":"Giuliani","year":"2011","journal-title":"Current Opinion in Cell Biology"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/j.ceb.2013.03.004","article-title":"Up-to-date membrane biogenesis in the autophagosome formation","volume":"25","author":"Hamasaki","year":"2013","journal-title":"Current Opinion in Cell Biology"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1073\/pnas.0701402104","article-title":"FIP200, a ULK-interacting protein, is required for autophagosome formation in mammalian cells","volume":"181","author":"Hara","year":"2008","journal-title":"The Journal of Cell Biology"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"9587","DOI":"10.1074\/jbc.M110.202911","article-title":"Autophagy controls IL-1 secretion by targeting pro-IL-1 for degradation","volume":"286","author":"Harris","year":"2011","journal-title":"Journal of Biological Chemistry"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1153","DOI":"10.1126\/science.1137395","article-title":"Far-field optical nanoscopy","volume":"316","author":"Hell","year":"2007","journal-title":"Science"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/j.bbrc.2005.07.070","article-title":"Regulation of exosome secretion by Rab35 and its GTPase-activating proteins TBC1D10A-c","volume":"189","author":"Hsu","year":"2010","journal-title":"The Journal of Cell Biology"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"810","DOI":"10.1126\/science.1153529","article-title":"Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy","volume":"319","author":"Huang","year":"2008","journal-title":"Science"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1047","DOI":"10.1016\/j.cell.2010.12.002","article-title":"Breaking the diffraction barrier: super-resolution imaging of cells","volume":"143","author":"Huang","year":"2010","journal-title":"Cell"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"10703","DOI":"10.1074\/jbc.M112.414771","article-title":"Chaperone-mediated autophagy targets hypoxia-inducible factor-1 (hIF-1\u00a0) for lysosomal degradation","volume":"288","author":"Hubbi","year":"2013","journal-title":"Journal of Biological Chemistry"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"795","DOI":"10.1126\/science.154.3753.1186","article-title":"Rab27a regulates the peripheral distribution of melanosomes in melanocytes","volume":"152","author":"Hume","year":"2001","journal-title":"The Journal of Cell Biology"},{"key":"bib33","first-page":"3584","article-title":"Serological cloning of a melanocyte rab guanosine 5'-triphosphate-binding protein and a chromosome condensation protein from a melanoma complementary DNA library","volume":"60","author":"J\u00e4ger","year":"2000","journal-title":"Cancer Research"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1159\/000346707","article-title":"Secretory versus degradative autophagy: unconventional secretion of inflammatory mediators","volume":"5","author":"Jiang","year":"2013","journal-title":"Journal of Innate Immunity"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.tcb.2012.05.006","article-title":"Chaperone-mediated autophagy: a unique way to enter the lysosome world","volume":"22","author":"Kaushik","year":"2012","journal-title":"Trends in Cell Biology"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"524","DOI":"10.1016\/j.cell.2007.06.029","article-title":"The golgi-associated protein GRASP is required for unconventional protein secretion during development","volume":"130","author":"Kinseth","year":"2007","journal-title":"Cell"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"4089","DOI":"10.1242\/jcs.156034","article-title":"Ultrastructural analysis of autophagosome organization using mammalian autophagy-deficient cells","volume":"127","author":"Kishi-Itakura","year":"2014","journal-title":"Journal of Cell Science"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1038\/nrm3696","article-title":"The autophagosome: origins unknown, biogenesis complex","volume":"14","author":"Lamb","year":"2013","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1146\/annurev.cellbio.20.010403.105307","article-title":"BI-DIRECTIONAL PROTEIN TRANSPORT BETWEEN the ER and GOLGI","volume":"20","author":"Lee","year":"2004","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1016\/j.cell.2007.07.030","article-title":"GRASPing unconventional secretion","volume":"130","author":"Levi","year":"2007","journal-title":"Cell"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"974","DOI":"10.1016\/j.celrep.2014.07.012","article-title":"Single-cell imaging of caspase-1 dynamics reveals an all-or-none inflammasome signaling response","volume":"8","author":"Liu","year":"2014","journal-title":"Cell Reports"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.cytogfr.2011.10.001","article-title":"Understanding the mechanism of IL-1 secretionn","volume":"22","author":"Lopez-Castejon","year":"2011","journal-title":"Cytokine & Growth Factor Reviews"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"825","DOI":"10.1016\/S1074-7613(01)00229-1","article-title":"Rapid secretion of interleukin-1 by microvesicle sheddingg","volume":"15","author":"Mackenzie","year":"2001","journal-title":"Immunity"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"2435","DOI":"10.1016\/j.biocel.2004.02.013","article-title":"Mechanisms of chaperone-mediated autophagy","volume":"36","author":"Majeski","year":"2004","journal-title":"The International Journal of Biochemistry & Cell Biology"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"537","DOI":"10.1073\/pnas.94.7.3070","article-title":"Unconventional secretion of pichia pastoris Acb1 is dependent on GRASP protein, peroxisomal functions, and autophagosome formation","volume":"188","author":"Manjithaya","year":"2010","journal-title":"The Journal of Cell Biology"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.tcb.2010.09.009","article-title":"Autophagy: a broad role in unconventional protein secretion?","volume":"21","author":"Manjithaya","year":"2011","journal-title":"Trends in Cell Biology"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1016\/0014-5793(93)80398-E","article-title":"Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells","volume":"152","author":"Mizushima","year":"2001","journal-title":"The Journal of Cell Biology"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"823","DOI":"10.1038\/ncb0910-823","article-title":"Autophagy in mammalian development and differentiation","volume":"12","author":"Mizushima","year":"2010","journal-title":"Nature Cell Biology"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1146\/annurev-cellbio-092910-154005","article-title":"The role of atg proteins in autophagosome formation","volume":"27","author":"Mizushima","year":"2011","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"1181","DOI":"10.1093\/carcin\/bgt111","article-title":"The HSP70 family and cancer","volume":"34","author":"Murphy","year":"2013","journal-title":"Carcinogenesis"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1038\/ni.1980","article-title":"Autophagy proteins regulate innate immune responses by inhibiting the release of mitochondrial DNA mediated by the NALP3 inflammasome","volume":"12","author":"Nakahira","year":"2011","journal-title":"Nature Immunology"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"2109","DOI":"10.1046\/j.1432-1033.2003.03577.x","article-title":"The mystery of nonclassical protein secretion","volume":"270","author":"Nickel","year":"2003","journal-title":"European Journal of Biochemistry"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1146\/annurev.cellbio.24.110707.175320","article-title":"Unconventional mechanisms of protein transport to the cell surface of eukaryotic cells","volume":"24","author":"Nickel","year":"2008","journal-title":"Annual Review of Cell and Developmental Biology"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"148","DOI":"10.1038\/nrm2617","article-title":"Mechanisms of regulated unconventional protein secretion","volume":"10","author":"Nickel","year":"2009","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1016\/j.copbio.2010.06.004","article-title":"Pathways of unconventional protein secretion","volume":"21","author":"Nickel","year":"2010","journal-title":"Current Opinion in Biotechnology"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1038\/ncb2000","article-title":"Rab27a and Rab27b control different steps of the exosome secretion pathway","volume":"12","author":"Ostrowski","year":"2010","journal-title":"Nature Cell Biology"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1038\/ncb1007-1102","article-title":"Unconventional secretion by autophagosome exocytosis","volume":"188","author":"Pfeffer","year":"2010","journal-title":"The Journal of Cell Biology"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"1913","DOI":"10.4049\/jimmunol.179.3.1913","article-title":"Nonclassical IL-1 secretion stimulated by P2X7 receptors is dependent on inflammasome activation and correlated with exosome release in murine macrophages","volume":"179","author":"Qu","year":"2007","journal-title":"The Journal of Immunology"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"5251","DOI":"10.1242\/jcs.103630","article-title":"Diversity in unconventional protein secretion","volume":"125","author":"Rabouille","year":"2012","journal-title":"Journal of Cell Science"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1038\/ni.2237","article-title":"Regulation of inflammasome signaling","volume":"13","author":"Rathinam","year":"2012","journal-title":"Nature Immunology"},{"key":"bib61","doi-asserted-by":"crossref","first-page":"1503","DOI":"10.1002\/j.1460-2075.1990.tb08268.x","article-title":"A novel secretory pathway for interleukin-1 beta, a protein lacking a signal sequence","volume":"9","author":"Rubartelli","year":"1990","journal-title":"The EMBO Journal"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"793","DOI":"10.1038\/nmeth929","article-title":"Sub-diffraction-limit imaging by stochastic optical reconstruction microscopy (sTORM)","volume":"3","author":"Rust","year":"2006","journal-title":"Nature Methods"},{"key":"bib63","doi-asserted-by":"crossref","first-page":"2505","DOI":"10.1242\/jcs.115.12.2505","article-title":"The exosome pathway in K562 cells is regulated by Rab11","volume":"115","author":"Savina","year":"2002","journal-title":"Journal of Cell Science"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1126\/science.271.5255.1519","article-title":"Common principles of protein translocation across membranes","volume":"271","author":"Schatz","year":"1996","journal-title":"Science"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1038\/ni.2215","article-title":"Activation of autophagy by inflammatory signals limits IL-1 production by targeting ubiquitinated inflammasomes for destructionn","volume":"13","author":"Shi","year":"2012","journal-title":"Nature Immunology"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"4736","DOI":"10.1038\/srep04736","article-title":"Real-time single-cell imaging of protein secretion","volume":"4","author":"Shirasaki","year":"2014","journal-title":"Scientific Reports"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1084\/jem.167.2.389","article-title":"Interleukin 1 beta is localized in the cytoplasmic ground substance but is largely absent from the golgi apparatus and plasma membranes of stimulated human monocytes","volume":"167","author":"Singer","year":"1988","journal-title":"Journal of Experimental Medicine"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"1202","DOI":"10.1016\/j.jmb.2014.07.012","article-title":"Unconventional secretion of fibroblast growth factor 2\u2014a novel type of protein translocation across membranes?","volume":"427","author":"Steringer","year":"2015","journal-title":"Journal of Molecular Biology"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/embor.2012.220","article-title":"Non-autophagic roles of autophagy-related proteins","volume":"14","author":"Subramani","year":"2013","journal-title":"EMBO Reports"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1038\/356768a0","article-title":"A novel heterodimeric cysteine protease is required for interleukin-1processing in monocytess","volume":"356","author":"Thornberry","year":"1992","journal-title":"Nature"},{"key":"bib71","doi-asserted-by":"publisher","first-page":"896","DOI":"10.1091\/mbc.E11-09-0785","article-title":"Mammalian Atg2 proteins are essential for autophagosome formation and important for regulation of size and distribution of lipid droplets","volume":"23","author":"Velikkakath","year":"2012","journal-title":"Molecular Biology of the Cell"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"5728","DOI":"10.4049\/jimmunol.170.11.5728","article-title":"P2X7 receptor-dependent blebbing and the activation of rho-effector kinases, caspases, and IL-1 release","volume":"170","author":"Verhoef","year":"2003","journal-title":"The Journal of Immunology"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1016\/j.gene.2012.11.002","article-title":"Interaction between hypertension and HSP70 variants increase the risk of cerebral ischemia in chinese han population: an association study","volume":"513","author":"Wei","year":"2013","journal-title":"Gene"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"1601","DOI":"10.1083\/jcb.115.6.1601","article-title":"Protein folding causes an arrest of preprotein translocation into mitochondria in vivo","volume":"115","author":"Wienhues","year":"1991","journal-title":"The Journal of Cell Biology"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.ceb.2009.11.014","article-title":"Mammalian autophagy: core molecular machinery and signaling regulation","volume":"22","author":"Yang","year":"2010","journal-title":"Current Opinion in Cell Biology"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"3960","DOI":"10.4049\/jimmunol.1001462","article-title":"P300-mediated acetylation stabilizes the th-inducing POK factor","volume":"185","author":"Zhang","year":"2010","journal-title":"The Journal of Immunology"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"559","DOI":"10.1126\/science.1234740","article-title":"Unconventional secretion, unconventional solutions","volume":"340","author":"Zhang","year":"2013","journal-title":"Science"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"805","DOI":"10.3892\/mmr.2011.528","article-title":"ShRNA-mediated gene silencing of heat shock protein 70 inhibits human colon cancer growth","volume":"4","author":"Zhong","year":"2011","journal-title":"Molecular Medicine Reports"},{"key":"bib79","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1038\/nature09663","article-title":"A role for mitochondria in NLRP3 inflammasome activation","volume":"469","author":"Zhou","year":"2011","journal-title":"Nature"},{"key":"bib80","doi-asserted-by":"publisher","first-page":"396","DOI":"10.1016\/j.bbrc.2012.04.032","article-title":"Short-hairpin RNA-mediated heat shock protein 90 gene silencing inhibits human breast cancer cell growth in vitro and in vivo","volume":"421","author":"Zuo","year":"2012","journal-title":"Biochemical and Biophysical Research Communications"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/11205\/elife-11205-v2.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/11205\/elife-11205-v2.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/11205","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T20:53:39Z","timestamp":1697057619000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/11205"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,11,2]]},"references-count":80,"alternative-id":["10.7554\/eLife.11205"],"URL":"https:\/\/doi.org\/10.7554\/elife.11205","archive":["CLOCKSS"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.725901413.793513220","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.11205.023","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.725901413.793512019","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.11205.022","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,11,2]]},"article-number":"e11205"}}