{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:35:59Z","timestamp":1790202959083,"version":"4.1.0"},"reference-count":31,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T00:00:00Z","timestamp":1360022400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T00:00:00Z","timestamp":1360022400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"},{"start":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T00:00:00Z","timestamp":1360022400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000011","name":"Howard Hughes Medical Institute","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100000011","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 CA093507"],"award-info":[{"award-number":["R01 CA093507"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"name":"American College of Rheumatology REF Physician Scientist Development Award"},{"name":"The Rockefeller University Hospital CTSA","award":["UL1RR024143"],"award-info":[{"award-number":["UL1RR024143"]}]},{"name":"The Rockefeller University Hospital CTSA","award":["8 KL2 TR000151"],"award-info":[{"award-number":["8 KL2 TR000151"]}]},{"name":"Beth and Ravenel Curry clinical scholarship"}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>FK506 (Tacrolimus) is a potent inhibitor of calcineurin that blocks IL2 production and is widely used to prevent transplant rejection and treat autoimmunity. FK506 treatment of dendritic cells (FKDC) limits their capacity to stimulate T cell responses. FK506 does not prevent DC survival, maturation, or costimulatory molecule expression, suggesting that the limited capacity of FKDC to stimulate T cells may be due to inhibition of calcineurin signaling in the DC. Instead, we demonstrate that DC inhibit T cells by sequestering FK506 and continuously releasing the drug over several days. T cells encountering FKDC proliferate but fail to upregulate the survival factor bcl-xl and die, and IL2 restores both bcl-xl and survival. In mice, FKDC act in an antigen-specific manner to inhibit T-cell mediated autoimmune arthritis. This establishes that DCs can act as a cellular drug delivery system to target antigen specific T cells.<\/jats:p>","DOI":"10.7554\/elife.00105","type":"journal-article","created":{"date-parts":[[2013,2,5]],"date-time":"2013-02-05T05:42:17Z","timestamp":1360042937000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.00105","source":"Crossref","is-referenced-by-count":22,"title":["Dendritic cells loaded with FK506 kill T cells in an antigen-specific manner and prevent autoimmunity in vivo"],"prefix":"10.7554","volume":"2","author":[{"given":"Dana E","family":"Orange","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Neuro-Oncology, The Rockefeller University, New York, United States"},{"name":"Division of Rheumatology, Hospital for Special Surgery, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie E","family":"Blachere","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuro-Oncology, The Rockefeller University, New York, United States"},{"name":"Howard Hughes Medical Institute, The Rockefeller University, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Fak","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuro-Oncology, The Rockefeller University, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salina","family":"Parveen","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuro-Oncology, The Rockefeller University, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mayu O","family":"Frank","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neuro-Oncology, The Rockefeller University, New York, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margo","family":"Herre","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular 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