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Here we show that the phospholipid remodeling enzyme lysophosphatidylcholine acyltransferase 3 (Lpcat3) is a critical determinant of triglyceride (TG) secretion due to its unique ability to catalyze the incorporation of arachidonate into membranes. Mice lacking\n                    <jats:italic>Lpcat3<\/jats:italic>\n                    in the intestine fail to thrive during weaning and exhibit enterocyte lipid accumulation and reduced plasma TGs. Mice lacking\n                    <jats:italic>Lpcat3<\/jats:italic>\n                    in the liver show reduced plasma TGs, hepatosteatosis, and secrete lipid-poor very low-density lipoprotein (VLDL) lacking arachidonoyl PLs. Mechanistic studies indicate that Lpcat3 activity impacts membrane lipid mobility in living cells, suggesting a biophysical basis for the requirement of arachidonoyl PLs in lipidating lipoprotein particles. These data identify Lpcat3 as a key factor in lipoprotein production and illustrate how manipulation of membrane composition can be used as a regulatory mechanism to control metabolic pathways.\n                  <\/jats:p>","DOI":"10.7554\/elife.06557","type":"journal-article","created":{"date-parts":[[2015,3,25]],"date-time":"2015-03-25T08:37:56Z","timestamp":1427272676000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.06557","source":"Crossref","is-referenced-by-count":205,"title":["Lpcat3-dependent production of arachidonoyl phospholipids is a key determinant of triglyceride secretion"],"prefix":"10.7554","volume":"4","author":[{"given":"Xin","family":"Rong","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Howard Hughes Medical Institute, University of California, Los Angeles, Los Angeles, United 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