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While no structures of a voltage-sensor toxin bound to a channel have been solved, a structural homolog, psalmotoxin (PcTx1), was recently crystalized in complex with the extracellular domain of an acid sensing ion channel (ASIC). In the present study we use spectroscopic, biophysical and computational approaches to compare membrane interaction properties and channel binding surfaces of PcTx1 with the voltage-sensor toxin guangxitoxin (GxTx-1E). Our results show that both types of tarantula toxins interact with membranes, but that voltage-sensor toxins partition deeper into the bilayer. In addition, our results suggest that tarantula toxins have evolved a similar concave surface for clamping onto \u03b1-helices that is effective in aqueous or lipidic physical environments.<\/jats:p>","DOI":"10.7554\/elife.06774","type":"journal-article","created":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T06:37:28Z","timestamp":1430980648000},"source":"Crossref","is-referenced-by-count":37,"title":["Tarantula toxins use common surfaces for interacting with Kv and ASIC ion channels"],"prefix":"10.7554","volume":"4","author":[{"given":"Kanchan","family":"Gupta","sequence":"first","affiliation":[{"name":"Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institutes of Health, Bethesda, United States"},{"name":"National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam","family":"Zamanian","sequence":"additional","affiliation":[{"name":"Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institutes of Health, Bethesda, United States"},{"name":"National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chanhyung","family":"Bae","sequence":"additional","affiliation":[{"name":"Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institutes of Health, Bethesda, United States"},{"name":"National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States"},{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mirela","family":"Milescu","sequence":"additional","affiliation":[{"name":"Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institutes of Health, Bethesda, United States"},{"name":"National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States"},{"name":"Biology Division, University of Missouri, Columbia, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dmitriy","family":"Krepkiy","sequence":"additional","affiliation":[{"name":"Molecular Physiology and Biophysics Section, Porter Neuroscience Research Center, National Institutes of Health, Bethesda, United States"},{"name":"National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Drew C","family":"Tilley","sequence":"additional","affiliation":[{"name":"Department of Physiology and Membrane Biology, University of California, Davis, Davis, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon T","family":"Sack","sequence":"additional","affiliation":[{"name":"Department of Physiology and Membrane Biology, University of California, Davis, Davis, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Yarov-Yarovoy","sequence":"additional","affiliation":[{"name":"Department of Physiology and Membrane Biology, University of California, Davis, Davis, United States"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jae Il","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenton 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