{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T16:45:55Z","timestamp":1775925955734,"version":"3.50.1"},"reference-count":31,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T00:00:00Z","timestamp":1434672000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T00:00:00Z","timestamp":1434672000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T00:00:00Z","timestamp":1434672000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China (NSFC)","doi-asserted-by":"publisher","award":["31101816"],"award-info":[{"award-number":["31101816"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Foot-and-mouth disease virus (FMDV) is an economically devastating viral disease leading to a substantial loss to the swine industry worldwide. A novel alternative strategy is to develop pigs that are genetically resistant to infection. Here, we produce transgenic (TG) pigs that constitutively expressed FMDV-specific short interfering RNA (siRNA) derived from small hairpin RNA (shRNA). In vitro challenge of TG fibroblasts showed the shRNA suppressed viral growth. TG and non-TG pigs were challenged by intramuscular injection with 100 LD50 of FMDV. High fever, severe clinical signs of foot-and-mouth disease and typical histopathological changes were observed in all of the non-TG pigs but in none of the high-siRNA pigs. Our results show that TG shRNA can provide a viable tool for producing animals with enhanced resistance to FMDV.<\/jats:p>","DOI":"10.7554\/elife.06951","type":"journal-article","created":{"date-parts":[[2015,6,19]],"date-time":"2015-06-19T07:46:29Z","timestamp":1434699989000},"source":"Crossref","is-referenced-by-count":36,"title":["Transgenic shRNA pigs reduce susceptibility to foot and mouth disease virus infection"],"prefix":"10.7554","volume":"4","author":[{"given":"Shengwei","family":"Hu","sequence":"first","affiliation":[{"name":"College of Life Sciences, Shihezi University, Shihezi, China"},{"name":"College of Animal Sciences, Shihezi University, Shihezi, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Qiao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Shihezi University, Shihezi, 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