{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T08:00:04Z","timestamp":1772265604745,"version":"3.50.1"},"reference-count":31,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009827","name":"Laura and John Arnold Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100009827","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The Reproducibility Project: Cancer Biology seeks to address growing concerns about reproducibility in scientific research by conducting replications of selected experiments from a number of high-profile papers in the field of cancer biology. The papers, which were published between 2010 and 2012, were selected on the basis of citations and Altmetric scores (&lt;xref ref-type=\"bibr\" rid=\"bib10\"&gt;Errington et al., 2014&lt;\/xref&gt;). This Registered report describes the proposed replication plan of key experiments from \u2018Inhibition of bromodomain and extra terminal (BET) recruitment to chromatin as an effective treatment for mixed-lineage leukemia (MLL)-fusion leukemia\u2019 by Dawson and colleagues, published in Nature in 2011 (&lt;xref ref-type=\"bibr\" rid=\"bib7\"&gt;Dawson et al., 2011&lt;\/xref&gt;). The experiments to be replicated are those reported in Figures 2A, 3D, 4B, 4D and Supplementary Figures 11A-B and 16A. In this study, BET proteins were demonstrated as potential therapeutic targets for modulating aberrant gene expression programs associated with MLL-fusion leukemia. In Figure 2A, the BET bromodomain inhibitor I-BET151 was reported to suppress growth of cells harboring MLL-fusions compared to those with alternate oncogenic drivers. In Figure 3D, treatment of MLL-fusion leukemia cells with I-BET151 resulted in transcriptional suppression of the anti-apoptotic gene BCL2. Figures 4B and 4D tested the therapeutic efficacy of I-BET151 in vivo using mice injected with human MLL-fusion leukemia cells and evaluated disease progression following I-BET151 treatment. The Reproducibility Project: Cancer Biology is a collaboration between the Center for Open Science and Science Exchange and the results of the replications will be published in eLife.<\/jats:p>","DOI":"10.7554\/elife.08997","type":"journal-article","created":{"date-parts":[[2015,8,29]],"date-time":"2015-08-29T03:35:22Z","timestamp":1440819322000},"source":"Crossref","is-referenced-by-count":6,"title":["Registered report: Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukemia"],"prefix":"10.7554","volume":"4","author":[{"given":"Juan Jos\u00e9","family":"Fung","sequence":"first","affiliation":[{"name":"ProNovus Bioscience, Mountain View, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan","family":"Kosaka","sequence":"additional","affiliation":[{"name":"ProNovus Bioscience, Mountain View, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaochuan","family":"Shan","sequence":"additional","affiliation":[{"name":"Stem Cell and Xenograft Core, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwenn","family":"Danet-Desnoyers","sequence":"additional","affiliation":[{"name":"Stem Cell and Xenograft Core, University of Pennsylvania, Perelman School of Medicine, Philadelphia, Pennsylvania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Gormally","sequence":"additional","affiliation":[{"name":"University of Cambridge, Cambridge, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"Owen","sequence":"additional","affiliation":[{"name":"University of Virginia, Charlottesville, Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Reproducibility Project: Cancer Biology","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"4374","published-online":{"date-parts":[[2015,9,1]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"697","DOI":"10.1182\/blood-2013-01-478420","article-title":"Potent antimyeloma activity of the novel bromodomain inhibitors I-BET151 and I-BET762","volume":"123","author":"Chaidos","year":"2014","journal-title":"Blood"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"303","DOI":"10.4103\/0976-500X.119726","article-title":"How to calculate sample size in animal studies?","volume":"4","author":"Charan","year":"2013","journal-title":"Journal of Pharmacology & Pharmacotherapeutics"},{"key":"bib3","doi-asserted-by":"crossref","first-page":"3230","DOI":"10.1182\/blood.V88.8.3230.bloodjournal8883230","article-title":"An experimental model of idiopathic pneumonia syndrome after bone marrow transplantation: I. 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