{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:03:01Z","timestamp":1790834581197,"version":"4.1.0"},"reference-count":58,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2015,9,30]],"date-time":"2015-09-30T00:00:00Z","timestamp":1443571200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,9,30]],"date-time":"2015-09-30T00:00:00Z","timestamp":1443571200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2015,9,30]],"date-time":"2015-09-30T00:00:00Z","timestamp":1443571200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01-Al1076434"],"award-info":[{"award-number":["R01-Al1076434"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of","award":["R01-DK078938"],"award-info":[{"award-number":["R01-DK078938"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Processes that define immunoglobulin repertoires are commonly presumed to be the same for all murine B cells. However, studies here that couple high-dimensional FACS sorting with large-scale quantitative IgH deep-sequencing demonstrate that B-1a IgH repertoire differs dramatically from the follicular and marginal zone B cells repertoires and is defined by distinct mechanisms. We track B-1a cells from their early appearance in neonatal spleen to their long-term residence in adult peritoneum and spleen. We show that de novo B-1a IgH rearrangement mainly occurs during the first few weeks of life, after which their repertoire continues to evolve profoundly, including convergent selection of certain V(D)J rearrangements encoding specific CDR3 peptides in all adults and progressive introduction of hypermutation and class-switching as animals age. This V(D)J selection and AID-mediated diversification operate comparably in germ-free and conventional mice, indicating these unique B-1a repertoire-defining mechanisms are driven by antigens that are not derived from microbiota.<\/jats:p>","DOI":"10.7554\/elife.09083","type":"journal-article","created":{"date-parts":[[2015,9,30]],"date-time":"2015-09-30T07:49:41Z","timestamp":1443599381000},"source":"Crossref","is-referenced-by-count":134,"title":["Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires"],"prefix":"10.7554","volume":"4","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"Genetics Department, Stanford University, Stanford, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlin","family":"Wang","sequence":"additional","affiliation":[{"name":"HudsonAlpha Institute for Biotechnology, Huntsville, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qunying","family":"Yang","sequence":"additional","affiliation":[{"name":"HudsonAlpha Institute for Biotechnology, Huntsville, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aaron B","family":"Kantor","sequence":"additional","affiliation":[{"name":"Genetics Department, Stanford University, Stanford, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiutung","family":"Chu","sequence":"additional","affiliation":[{"name":"Biology and Biological Engineering Department, California Institute of Technology, Pasadena, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliver EB","family":"Ghosn","sequence":"additional","affiliation":[{"name":"Genetics Department, Stanford University, Stanford, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guang","family":"Qin","sequence":"additional","affiliation":[{"name":"Genetics Department, Stanford University, Stanford, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarkis K","family":"Mazmanian","sequence":"additional","affiliation":[{"name":"Biology and Biological Engineering Department, California Institute of Technology, Pasadena, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Han","sequence":"additional","affiliation":[{"name":"HudsonAlpha Institute for Biotechnology, Huntsville, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonore A","family":"Herzenberg","sequence":"additional","affiliation":[{"name":"Genetics Department, Stanford University, Stanford, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2015,9,30]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"13700","DOI":"10.1073\/pnas.1107172108","article-title":"Reduced production of B-1-specified common lymphoid progenitors results in diminished potential of adult marrow to generate B-1 cells","volume":"108","author":"Barber","year":"2011","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/S1074-7613(00)80418-5","article-title":"B-1 and B-2 cell-derived immunoglobulin m antibodies are nonredundant components of the protective response to influenza virus infection","volume":"192","author":"Baumgarth","year":"2000","journal-title":"The Journal of Experimental Medicine"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1007\/s00281-004-0182-2","article-title":"Inherent specificities in natural antibodies: a key to immune defense against pathogen invasion","volume":"26","author":"Baumgarth","year":"2005","journal-title":"Springer Seminars in Immunopathology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"34","DOI":"10.1038\/nri2901","article-title":"The double life of a B-1 cell: self-reactivity selects for protective effector functions","volume":"11","author":"Baumgarth","year":"2011","journal-title":"Nature Reviews. 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