{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T02:57:50Z","timestamp":1789441070103,"version":"build-2803163510"},"reference-count":155,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM153408"],"award-info":[{"award-number":["R35GM153408"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM133764"],"award-info":[{"award-number":["R35GM133764"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R21AI110712"],"award-info":[{"award-number":["R21AI110712"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["T32DK007115"],"award-info":[{"award-number":["T32DK007115"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000861","name":"Burroughs Wellcome Fund","doi-asserted-by":"publisher","award":["1011969"],"award-info":[{"award-number":["1011969"]}],"id":[{"id":"10.13039\/100000861","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000875","name":"Pew Charitable Trusts","doi-asserted-by":"publisher","award":["32099"],"award-info":[{"award-number":["32099"]}],"id":[{"id":"10.13039\/100000875","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000051","name":"National Human Genome Research Institute","doi-asserted-by":"publisher","award":["R25HG009886"],"award-info":[{"award-number":["R25HG009886"]}],"id":[{"id":"10.13039\/100000051","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Malaria parasites have evolved unusual metabolic adaptations that specialize them for growth within heme-rich human erythrocytes. During blood-stage infection,\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    parasites internalize and digest abundant host hemoglobin within the digestive vacuole. This massive catabolic process generates copious free heme, most of which is biomineralized into inert hemozoin. Parasites also express a divergent heme oxygenase (HO)-like protein (PfHO) that lacks key active-site residues and has lost canonical HO activity. The cellular role of this unusual protein that underpins its retention by parasites has been unknown. To unravel PfHO function, we first determined a 2.8 \u00c5-resolution X-ray structure that revealed a highly \u03b1-helical fold indicative of distant HO homology. Localization studies unveiled PfHO targeting to the apicoplast organelle, where it is imported and undergoes N-terminal processing but retains most of the electropositive transit peptide. We observed that conditional knockdown of PfHO was lethal to parasites, which died from defective apicoplast biogenesis and impaired isoprenoid-precursor synthesis. Complementation and molecular-interaction studies revealed an essential role for the electropositive N-terminus of PfHO, which selectively associates with the apicoplast genome and enzymes involved in nucleic acid metabolism and gene expression. PfHO knockdown resulted in a specific deficiency in levels of apicoplast-encoded RNA but not DNA. These studies reveal an essential function for PfHO in apicoplast maintenance and suggest that\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    repurposed the conserved HO scaffold from its canonical heme-degrading function in the ancestral chloroplast to fulfill a critical adaptive role in organelle gene expression.\n                  <\/jats:p>","DOI":"10.7554\/elife.100256","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T12:26:00Z","timestamp":1724761560000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.100256","source":"Crossref","is-referenced-by-count":6,"title":["Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7473-5822","authenticated-orcid":true,"given":"Amanda Mixon","family":"Blackwell","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03r0ha626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, University of Utah School of Medicine","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8289-2222","authenticated-orcid":true,"given":"Yasaman","family":"Jami-Alahmadi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Chemistry, University of California, Los Angeles","place":["Los Angeles, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Armiyaw S","family":"Nasamu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036c27j91","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Medicine and Molecular Microbiology, Washington University School of Medicine","place":["St. Louis, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Shota","family":"Kudo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry & Biotechnology, The University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Akinobu","family":"Senoo","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00p4k0j84","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Protein Drug Discovery, Graduate School of Pharmaceutical Sciences, Kyushu University","place":["Fukuoka, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Celine","family":"Slam","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03r0ha626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, University of Utah School of Medicine","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Kouhei","family":"Tsumoto","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry & Biotechnology, The University of Tokyo","place":["Tokyo, Japan"]},{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bioengineering, University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]},{"given":"James A","family":"Wohlschlegel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/046rm7j60","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biological Chemistry, University of California, Los Angeles","place":["Los Angeles, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"given":"Jose","family":"Manuel Martinez Caaveiro","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry & Biotechnology, The University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3529-8399","authenticated-orcid":true,"given":"Daniel E","family":"Goldberg","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036c27j91","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Medicine and Molecular Microbiology, Washington University School of Medicine","place":["St. Louis, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3464-3042","authenticated-orcid":true,"given":"Paul A","family":"Sigala","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03r0ha626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, University of Utah School of Medicine","place":["Salt Lake City, United States"]},{"id":[{"id":"https:\/\/ror.org\/036c27j91","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Medicine and Molecular Microbiology, Washington University School of Medicine","place":["St. Louis, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2024,12,11]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"E2068","DOI":"10.1073\/pnas.1615195114","type":"journal-article","article-title":"Quantification of labile heme in live malaria parasites using a genetically encoded biosensor","volume":"114","author":"Abshire","year":"2017","journal-title":"PNAS"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"213","DOI":"10.1107\/S0907444909052925","type":"journal-article","article-title":"PHENIX: a comprehensive Python-based system for macromolecular structure solution","volume":"66","author":"Adams","year":"2010","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"bib3","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006754","type":"journal-article","article-title":"Transcription facilitated genome-wide recruitment of topoisomerase I and DNA gyrase","volume":"13","author":"Ahmed","year":"2017","journal-title":"PLOS Genetics"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"420","DOI":"10.1038\/s41587-019-0036-z","type":"journal-article","article-title":"SignalP 5.0 improves signal peptide predictions using deep neural networks","volume":"37","author":"Almagro Armenteros","year":"2019","journal-title":"Nature Biotechnology"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","type":"journal-article","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"Journal of Molecular Biology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"D898","DOI":"10.1093\/nar\/gkab929","type":"journal-article","article-title":"VEuPathDB: the eukaryotic pathogen, vector and host bioinformatics resource center","volume":"50","author":"Amos","year":"2022","journal-title":"Nucleic Acids Research"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"1744","DOI":"10.1093\/pcp\/pcr108","type":"journal-article","article-title":"A heme oxygenase isoform is essential for aerobic growth in the cyanobacterium synechocystis sp. PCC 6803: modes of differential operation of two isoforms\/enzymes to adapt to low oxygen environments in cyanobacteria","volume":"52","author":"Aoki","year":"2011","journal-title":"Plant and Cell Physiology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"1007","DOI":"10.1038\/nmeth1132","type":"journal-article","article-title":"An FKBP destabilization domain modulates protein levels in Plasmodium falciparum","volume":"4","author":"Armstrong","year":"2007","journal-title":"Nature Methods"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"6235","DOI":"10.1073\/pnas.1203516109","type":"journal-article","article-title":"Gene selective mRNA cleavage inhibits the development of Plasmodium falciparum","volume":"109","author":"Augagneur","year":"2012","journal-title":"PNAS"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"10037","DOI":"10.1073\/pnas.181342398","type":"journal-article","article-title":"Electrostatics of nanosystems: application to microtubules and the ribosome","volume":"98","author":"Baker","year":"2001","journal-title":"PNAS"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1016\/S0021-9258(18)57175-1","type":"journal-article","article-title":"Studies on malarial parasites","volume":"175","author":"Ball","year":"1948","journal-title":"Journal of Biological Chemistry"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"16391","DOI":"10.1073\/pnas.0504679102","type":"journal-article","article-title":"High-efficiency transformation of Plasmodium falciparum by the lepidopteran transposable element piggyBac","volume":"102","author":"Balu","year":"2005","journal-title":"PNAS"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"D523","DOI":"10.1093\/nar\/gkac1052","type":"journal-article","article-title":"UniProt: the universal protein knowledgebase in 2023","volume":"51","author":"Bateman","year":"2023","journal-title":"Nucleic Acids Research"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"592","DOI":"10.1038\/nature13574","type":"journal-article","article-title":"PTEX component HSP101 mediates export of diverse malaria effectors into host erythrocytes","volume":"511","author":"Beck","year":"2014","journal-title":"Nature"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"59","DOI":"10.1038\/embor.2009.232","type":"journal-article","article-title":"Coordination of genomic structure and transcription by the main bacterial nucleoid-associated protein HU","volume":"11","author":"Berger","year":"2010","journal-title":"EMBO Reports"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"26882","DOI":"10.1074\/jbc.M114.567685","type":"journal-article","article-title":"Nuclear heme oxygenase-1 (HO-1) modulates subcellular distribution and activation of Nrf2, impacting metabolic and anti-oxidant defenses","volume":"289","author":"Biswas","year":"2014","journal-title":"The Journal of Biological Chemistry"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/0166-6851(95)02483-2","type":"journal-article","article-title":"Restriction enzyme-mediated integration elevates transformation frequency and enables co-transfection of Toxoplasma gondii","volume":"74","author":"Black","year":"1995","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib18","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49396-3","type":"journal-article","article-title":"Non-canonical transcriptional regulation of heme oxygenase in Aedes aegypti","volume":"9","author":"Bottino-Rojas","year":"2019","journal-title":"Scientific Reports"},{"key":"bib19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2005895","type":"journal-article","article-title":"Integrative proteomics and bioinformatic prediction enable a high-confidence apicoplast proteome in malaria parasites","volume":"16","author":"Boucher","year":"2018","journal-title":"PLOS Biology"},{"key":"bib20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007661","type":"journal-article","article-title":"Plastid-endomembrane connections in apicomplexan parasites","volume":"15","author":"Boucher","year":"2019","journal-title":"PLOS Pathogens"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"128","DOI":"10.1016\/j.molbiopara.2007.06.011","type":"journal-article","article-title":"Expression and biochemical characterization of Plasmodium falciparum DNA ligase I","volume":"155","author":"Buguliskis","year":"2007","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"260","DOI":"10.1016\/j.cell.2017.06.030","type":"journal-article","article-title":"Functional profiling of a plasmodium genome reveals an abundance of essential genes","volume":"170","author":"Bushell","year":"2017","journal-title":"Cell"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"369","DOI":"10.1146\/annurev.biochem.70.1.369","type":"journal-article","article-title":"DNA topoisomerases: structure, function, and mechanism","volume":"70","author":"Champoux","year":"2001","journal-title":"Annual Review of Biochemistry"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"3389","DOI":"10.1128\/AAC.02711-13","type":"journal-article","article-title":"Sulfur mobilization for Fe-S cluster assembly by the essential SUF pathway in the Plasmodium falciparum apicoplast and its inhibition","volume":"58","author":"Charan","year":"2014","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"3124","DOI":"10.1128\/AAC.00394-06","type":"journal-article","article-title":"Tetracyclines specifically target the apicoplast of the malaria parasite Plasmodium falciparum","volume":"50","author":"Dahl","year":"2006","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"3485","DOI":"10.1128\/AAC.00527-07","type":"journal-article","article-title":"Multiple antibiotics exert delayed effects against the Plasmodium falciparum apicoplast","volume":"51","author":"Dahl","year":"2007","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1016\/j.pt.2008.03.007","type":"journal-article","article-title":"Apicoplast translation, transcription and genome replication: targets for antimalarial antibiotics","volume":"24","author":"Dahl","year":"2008","journal-title":"Trends in Parasitology"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"656","DOI":"10.1104\/pp.126.2.656","type":"journal-article","article-title":"The heme-oxygenase family required for phytochrome chromophore biosynthesis is necessary for proper photomorphogenesis in higher plants","volume":"126","author":"Davis","year":"2001","journal-title":"Plant Physiology"},{"key":"bib29","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2016.0181","type":"journal-article","article-title":"Structure and dynamics of bacterial ribosome biogenesis","volume":"372","author":"Davis","year":"2017","journal-title":"Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"1743","DOI":"10.1089\/ars.2013.5674","type":"journal-article","article-title":"Signaling function of heme oxygenase proteins","volume":"20","author":"Dennery","year":"2014","journal-title":"Antioxidants & Redox Signaling"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"155","DOI":"10.1006\/jmbi.1996.0449","type":"journal-article","article-title":"Complete gene map of the plastid-like DNA of the malaria parasite Plasmodium falciparum","volume":"261","author":"Denny","year":"1996","journal-title":"Journal of Molecular Biology"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1038\/nrmicro2261","type":"journal-article","article-title":"Bacterial nucleoid-associated proteins, nucleoid structure and gene expression","volume":"8","author":"Dillon","year":"2010","journal-title":"Nature Reviews Microbiology"},{"key":"bib33","doi-asserted-by":"publisher","DOI":"10.1186\/s12860-019-0211-6","type":"journal-article","article-title":"DNA supercoiling and transcription in bacteria: a two-way street","volume":"20","author":"Dorman","year":"2019","journal-title":"BMC Molecular and Cell Biology"},{"key":"bib34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004008","type":"journal-article","article-title":"Evolution of chloroplast transcript processing in Plasmodium and its chromerid algal relatives","volume":"10","author":"Dorrell","year":"2014","journal-title":"PLOS Genetics"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"456","DOI":"10.1016\/j.jmb.2010.09.051","type":"journal-article","article-title":"The Clp chaperones and proteases of the human malaria parasite Plasmodium falciparum","volume":"404","author":"El Bakkouri","year":"2010","journal-title":"Journal of Molecular Biology"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"438","DOI":"10.1111\/paa.1999.111.5.438","type":"journal-article","article-title":"Heme oxygenase: recent advances in understanding its regulation and role","volume":"111","author":"Elbirt","year":"1999","journal-title":"Proceedings of the Association of American Physicians"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"856","DOI":"10.1104\/pp.105.074211","type":"journal-article","article-title":"Multiple heme oxygenase family members contribute to the biosynthesis of the phytochrome chromophore in Arabidopsis","volume":"140","author":"Emborg","year":"2006","journal-title":"Plant Physiology"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1107\/S0907444910007493","type":"journal-article","article-title":"Features and development of Coot","volume":"66","author":"Emsley","year":"2010","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2301047120","type":"journal-article","article-title":"Direct tests of cytochrome c and C1 functions in the electron transport chain of malaria parasites","volume":"120","author":"Espino-Sanchez","year":"2023","journal-title":"PNAS"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1107\/S0907444905036693","type":"journal-article","article-title":"Scaling and assessment of data quality","volume":"62","author":"Evans","year":"2006","journal-title":"Acta Crystallographica Section D Biological Crystallography"},{"key":"bib41","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.71636","type":"journal-article","article-title":"Divergent acyl carrier protein decouples mitochondrial Fe-S cluster biogenesis from fatty acid synthesis in malaria parasites","volume":"10","author":"Falekun","year":"2021","journal-title":"eLife"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"430","DOI":"10.1006\/expr.1995.1055","type":"journal-article","article-title":"Plasmodium falciparum: alterations in organelle transcript abundance during the erythrocytic cycle","volume":"80","author":"Feagin","year":"1995","journal-title":"Experimental Parasitology"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"10931","DOI":"10.1073\/pnas.94.20.10931","type":"journal-article","article-title":"Transformation with human dihydrofolate reductase renders malaria parasites insensitive to WR99210 but does not affect the intrinsic activity of proguanil","volume":"94","author":"Fidock","year":"1997","journal-title":"PNAS"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"1746","DOI":"10.1016\/j.celrep.2017.10.081","type":"journal-article","article-title":"PfClpC Is an Essential Clp chaperone required for plastid integrity and Clp protease stability in Plasmodium falciparum","volume":"21","author":"Florentin","year":"2017","journal-title":"Cell Reports"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1146\/annurev.micro.51.1.97","type":"journal-article","article-title":"Hemoglobin metabolism in the malaria parasite Plasmodium falciparum","volume":"51","author":"Francis","year":"1997","journal-title":"Annual Review of Microbiology"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"575","DOI":"10.1002\/prot.22582","type":"journal-article","article-title":"Plasmodium falciparum acyl carrier protein crystal structures in disulfide-linked and reduced states and their prevalence during blood stage growth","volume":"78","author":"Gallagher","year":"2010","journal-title":"Proteins"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"1124","DOI":"10.1111\/j.1600-0854.2011.01232.x","type":"journal-article","article-title":"Plasmodium falciparum apicoplast transit peptides are unstructured in vitro and during apicoplast import","volume":"12","author":"Gallagher","year":"2011","journal-title":"Traffic"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/j.molbiopara.2011.01.004","type":"journal-article","article-title":"Yeast dihydroorotate dehydrogenase as a new selectable marker for Plasmodium falciparum transfection","volume":"177","author":"Ganesan","year":"2011","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms10727","type":"journal-article","article-title":"Synthetic RNA-protein modules integrated with native translation mechanisms to control gene expression in malaria parasites","volume":"7","author":"Ganesan","year":"2016","journal-title":"Nature Communications"},{"key":"bib50","doi-asserted-by":"publisher","DOI":"10.1101\/2024.02.01.575742","type":"preprint","article-title":"Biogenesis of Cytochromes c and C1 in the electron transport chain of malaria parasites","volume-title":"bioRxiv","author":"Garc\u00eda-Guerrero","year":"2024"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1042\/BJ20090775","type":"journal-article","article-title":"Characterization of the haem oxygenase protein family in Arabidopsis thaliana reveals a diversity of functions","volume":"425","author":"Gisk","year":"2010","journal-title":"Biochemical Journal"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"2931","DOI":"10.1073\/pnas.87.8.2931","type":"journal-article","article-title":"Hemoglobin degradation in the malaria parasite Plasmodium falciparum: an ordered process in a unique organelle","volume":"87","author":"Goldberg","year":"1990","journal-title":"PNAS"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6329","type":"journal-article","article-title":"Versatile control of Plasmodium falciparum gene expression with an inducible protein-RNA interaction","volume":"5","author":"Goldfless","year":"2014","journal-title":"Nature Communications"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1141","DOI":"10.1016\/j.bbrc.2006.02.195","type":"journal-article","article-title":"A complex of DNA gyrase and RNA polymerase fosters transcription in Mycobacterium smegmatis","volume":"343","author":"Gupta","year":"2006","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"1711","DOI":"10.1016\/j.molcel.2024.03.006","type":"journal-article","article-title":"DDX21 mediates co-transcriptional RNA m6A modification to promote transcription termination and genome stability","volume":"84","author":"Hao","year":"2024","journal-title":"Molecular Cell"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/978-1-0716-0270-6_3","type":"journal-article","article-title":"Using dali for protein structure comparison","volume":"2112","author":"Holm","year":"2020","journal-title":"Methods in Molecular Biology"},{"key":"bib57","doi-asserted-by":"publisher","DOI":"10.1002\/pro.4519","type":"journal-article","article-title":"DALI shines a light on remote homologs: One hundred discoveries","volume":"32","author":"Holm","year":"2023","journal-title":"Protein Science"},{"key":"bib58","doi-asserted-by":"publisher","first-page":"6805","DOI":"10.1038\/onc.2017.294","type":"journal-article","article-title":"Acetylation is essential for nuclear heme oxygenase-1-enhanced tumor growth and invasiveness","volume":"36","author":"Hsu","year":"2017","journal-title":"Oncogene"},{"key":"bib59","doi-asserted-by":"publisher","first-page":"7","DOI":"10.15430\/JCP.2022.27.1.7","type":"journal-article","article-title":"Non-canonical vs. canonical functions of heme oxygenase-1 in cancer","volume":"27","author":"Jagadeesh","year":"2022","journal-title":"Journal of Cancer Prevention"},{"key":"bib60","doi-asserted-by":"publisher","DOI":"10.3791\/62380","type":"journal-article","article-title":"A robust method for packing high resolution C18 RP-nano-HPLC Columns","volume":"01","author":"Jami-Alahmadi","year":"2021","journal-title":"Journal of Visualized Experiments"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"10200","DOI":"10.1073\/pnas.1423790112","type":"journal-article","article-title":"Factors mediating plastid dependency and the origins of parasitism in apicomplexans and their close relatives","volume":"112","author":"Janou\u0161kovec","year":"2015","journal-title":"PNAS"},{"key":"bib62","doi-asserted-by":"publisher","first-page":"10941","DOI":"10.1093\/nar\/gkx772","type":"journal-article","article-title":"The FASTK family of proteins: emerging regulators of mitochondrial RNA biology","volume":"45","author":"Jourdain","year":"2017","journal-title":"Nucleic Acids Research"},{"key":"bib63","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","type":"journal-article","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1002\/pro.3280","type":"journal-article","article-title":"Improvements to the APBS biomolecular solvation software suite","volume":"27","author":"Jurrus","year":"2018","journal-title":"Protein Science"},{"key":"bib65","doi-asserted-by":"publisher","first-page":"1027","DOI":"10.1016\/j.jmb.2004.03.016","type":"journal-article","article-title":"A combined transmembrane topology and signal peptide prediction method","volume":"338","author":"K\u00e4ll","year":"2004","journal-title":"Journal of Molecular Biology"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1146\/annurev-arplant-050312-120144","type":"journal-article","article-title":"The number, speed, and impact of plastid endosymbioses in eukaryotic evolution","volume":"64","author":"Keeling","year":"2013","journal-title":"Annual Review of Plant Biology"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1083\/jcb200307147","type":"journal-article","article-title":"Trafficking of plasmepsin II to the food vacuole of the malaria parasite Plasmodium falciparum","volume":"164","author":"Klemba","year":"2004","journal-title":"The Journal of Cell Biology"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"382","DOI":"10.1111\/febs.15445","type":"journal-article","article-title":"Supply and demand-heme synthesis, salvage and utilization by Apicomplexa","volume":"288","author":"Kloehn","year":"2021","journal-title":"The FEBS Journal"},{"key":"bib69","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2214765120","type":"journal-article","article-title":"Coordination of apicoplast transcription in a malaria parasite by internal and host cues","volume":"120","author":"Kobayashi","year":"2023","journal-title":"PNAS"},{"key":"bib70","doi-asserted-by":"publisher","first-page":"1485","DOI":"10.1126\/science.275.5305.1485","type":"journal-article","article-title":"A plastid of probable green algal origin in apicomplexan parasites","volume":"275","author":"K\u00f6hler","year":"1997","journal-title":"Science"},{"key":"bib71","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.18357","type":"journal-article","article-title":"The aspartyl protease DDI2 activates Nrf1 to compensate for proteasome dysfunction","volume":"5","author":"Koizumi","year":"2016","journal-title":"eLife"},{"key":"bib72","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010094","type":"journal-article","article-title":"Proximity ligation assay detection of protein-DNA interactions-is there a link between heme oxygenase-1 and G-quadruplexes?","volume":"10","author":"Krzeptowski","year":"2021","journal-title":"Antioxidants"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1007\/s00775-004-0620-6","type":"journal-article","article-title":"Crystal structures of the G139A, G139A?NO and G143H mutants of human heme oxygenase-1. A finely tuned hydrogen-bonding network controls oxygenase versus peroxidase activity","volume":"10","author":"Lad","year":"2005","journal-title":"JBIC Journal of Biological Inorganic Chemistry"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1107\/S0021889892009944","type":"journal-article","article-title":"PROCHECK: A program to check the stereochemical quality of protein structures","volume":"26","author":"Laskowski","year":"1993","journal-title":"Journal of Applied Crystallography"},{"key":"bib75","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.17721","type":"journal-article","article-title":"Proteasome dysfunction triggers activation of SKN-1A\/Nrf1 by the aspartic protease DDI-1","volume":"5","author":"Lehrbach","year":"2016","journal-title":"eLife"},{"key":"bib76","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-12-25","type":"journal-article","article-title":"Cryo-electron tomography reveals four-membrane architecture of the Plasmodium apicoplast","volume":"12","author":"Lemgruber","year":"2013","journal-title":"Malaria Journal"},{"key":"bib77","doi-asserted-by":"publisher","first-page":"20621","DOI":"10.1074\/jbc.M607954200","type":"journal-article","article-title":"Heme oxygenase-1 protein localizes to the nucleus and activates transcription factors important in oxidative stress","volume":"282","author":"Lin","year":"2007","journal-title":"The Journal of Biological Chemistry"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.molbiopara.2011.08.002","type":"journal-article","article-title":"The primase domain of PfPrex is a proteolytically matured, essential enzyme of the apicoplast","volume":"180","author":"Lindner","year":"2011","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib79","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1074\/jbc.M409740200","type":"journal-article","article-title":"The role of Plasmodium falciparum food vacuole plasmepsins","volume":"280","author":"Liu","year":"2005","journal-title":"The Journal of Biological Chemistry"},{"key":"bib80","doi-asserted-by":"publisher","first-page":"1166","DOI":"10.1093\/nar\/gkj517","type":"journal-article","article-title":"Comparative whole genome transcriptome analysis of three Plasmodium falciparum strains","volume":"34","author":"Llin\u00e1s","year":"2006","journal-title":"Nucleic Acids Research"},{"key":"bib81","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-452","type":"journal-article","article-title":"Artemisinin resistance--modelling the potential human and economic costs","volume":"13","author":"Lubell","year":"2014","journal-title":"Malaria Journal"},{"key":"bib82","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009301","type":"journal-article","article-title":"Complexome profile of Toxoplasma gondii mitochondria identifies divergent subunits of respiratory chain complexes including new subunits of cytochrome bc1 complex","volume":"17","author":"Maclean","year":"2021","journal-title":"PLOS Pathogens"},{"key":"bib83","doi-asserted-by":"publisher","first-page":"30025","DOI":"10.1074\/jbc.M114.586735","type":"journal-article","article-title":"Kinase-associated endopeptidase 1 (Kae1) participates in an atypical ribosome-associated complex in the apicoplast of Plasmodium falciparum","volume":"289","author":"Mallari","year":"2014","journal-title":"The Journal of Biological Chemistry"},{"key":"bib84","doi-asserted-by":"publisher","first-page":"8716","DOI":"10.1073\/pnas.96.15.8716","type":"journal-article","article-title":"A set of independent selectable markers for transfection of the human malaria parasite Plasmodium falciparum","volume":"96","author":"Mamoun","year":"1999","journal-title":"PNAS"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"973","DOI":"10.1046\/j.1365-2958.2001.02289.x","type":"journal-article","article-title":"Plasmodium protein phosphatase 2C dephosphorylates translation elongation factor 1\u03b2 and inhibits its PKC\u2010mediated nucleotide exchange activity in vitro","volume":"39","author":"Mamoun","year":"2001","journal-title":"Molecular Microbiology"},{"key":"bib86","doi-asserted-by":"publisher","first-page":"493","DOI":"10.1016\/j.ijpara.2020.11.004","type":"journal-article","article-title":"Replication and partitioning of the apicoplast genome of Toxoplasma gondii is linked to the cell cycle and requires DNA polymerase and gyrase","volume":"51","author":"Martins-Duarte","year":"2021","journal-title":"International Journal for Parasitology"},{"key":"bib87","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00579-20","type":"journal-article","article-title":"Dispensable role of mitochondrial fission protein 1 (Fis1) in the erythrocytic development of Plasmodium falciparum","volume":"5","author":"Maruthi","year":"2020","journal-title":"mSphere"},{"key":"bib88","doi-asserted-by":"publisher","DOI":"10.3390\/antiox10010087","type":"journal-article","article-title":"Nuclear localization of heme oxygenase-1 in pathophysiological conditions: does it explain the dual role in cancer?","volume":"10","author":"Mascar\u00f3","year":"2021","journal-title":"Antioxidants"},{"key":"bib89","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1107\/S0021889807021206","type":"journal-article","article-title":"Phaser crystallographic software","volume":"40","author":"McCoy","year":"2007","journal-title":"Journal of Applied Crystallography"},{"key":"bib90","doi-asserted-by":"publisher","DOI":"10.1038\/381482a0","type":"journal-article","article-title":"Plastid in human parasites","volume":"381","author":"McFadden","year":"1996","journal-title":"Nature"},{"key":"bib91","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1016\/j.molbiopara.2016.06.006","type":"journal-article","article-title":"Replication and maintenance of the Plasmodium falciparum apicoplast genome","volume":"208","author":"Milton","year":"2016","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib92","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20381-z","type":"journal-article","article-title":"ATP synthase hexamer assemblies shape cristae of Toxoplasma mitochondria","volume":"12","author":"M\u00fchleip","year":"2021","journal-title":"Nature Communications"},{"key":"bib93","doi-asserted-by":"publisher","first-page":"4411","DOI":"10.1073\/pnas.1018449108","type":"journal-article","article-title":"Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable fluorescent affinity tag","volume":"108","author":"Muralidharan","year":"2011","journal-title":"PNAS"},{"key":"bib94","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1107\/S0907444996012255","type":"journal-article","article-title":"Refinement of macromolecular structures by the maximum-likelihood method","volume":"53","author":"Murshudov","year":"1997","journal-title":"Acta Crystallographica Section D Biological Crystallography"},{"key":"bib95","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-77644-4","type":"journal-article","article-title":"An integrated platform for genome engineering and gene expression perturbation in Plasmodium falciparum","volume":"11","author":"Nasamu","year":"2021","journal-title":"Scientific Reports"},{"key":"bib96","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2019.104275","type":"journal-article","article-title":"Development of artemisinin resistance in malaria therapy","volume":"146","author":"Na\u00df","year":"2019","journal-title":"Pharmacological Research"},{"key":"bib97","doi-asserted-by":"publisher","first-page":"377","DOI":"10.1016\/j.protis.2016.06.003","type":"journal-article","article-title":"Transcripts in the Plasmodium Apicoplast undergo cleavage at tRNAs and editing, and include antisense sequences","volume":"167","author":"Nisbet","year":"2016","journal-title":"Protist"},{"key":"bib98","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.molbiopara.2016.07.004","type":"journal-article","article-title":"Transcription of the apicoplast genome","volume":"210","author":"Nisbet","year":"2016","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib99","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004367","type":"journal-article","article-title":"The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction","volume":"4","author":"Oberto","year":"2009","journal-title":"PLOS ONE"},{"key":"bib100","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1016\/B978-0-12-407694-5.00008-0","type":"book-chapter","article-title":"Chapter eight - cell biology of chromerids: autotrophic relatives to apicomplexan parasites","volume-title":"International Review of Cell and Molecular Biology","author":"Oborn\u00edk","year":"2013"},{"key":"bib101","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1038\/290470a0","type":"journal-article","article-title":"tRNA punctuation model of RNA processing in human mitochondria","volume":"290","author":"Ojala","year":"1981","journal-title":"Nature"},{"key":"bib102","doi-asserted-by":"publisher","first-page":"313","DOI":"10.1016\/j.febslet.2008.12.015","type":"journal-article","article-title":"The novel heme oxygenase-like protein from Plasmodium falciparum converts heme to bilirubin IXalpha in the apicoplast","volume":"583","author":"Okada","year":"2009","journal-title":"FEBS Letters"},{"key":"bib103","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.73208","type":"journal-article","article-title":"Critical role for isoprenoids in apicoplast biogenesis by malaria parasites","volume":"11","author":"Okada","year":"2022","journal-title":"eLife"},{"key":"bib104","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1011713","type":"journal-article","article-title":"The interdependence of isoprenoid synthesis and apicoplast biogenesis in malaria parasites","volume":"19","author":"Okada","year":"2023","journal-title":"PLOS Pathogens"},{"key":"bib105","doi-asserted-by":"publisher","first-page":"4235","DOI":"10.1093\/nar\/8.18.4235","type":"journal-article","article-title":"Involvement of DNA gyrase in the transcription of ribosomal RNA","volume":"8","author":"Oostra","year":"1980","journal-title":"Nucleic Acids Research"},{"key":"bib106","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04966-3","type":"journal-article","article-title":"Genome-wide real-time in vivo transcriptional dynamics during Plasmodium falciparum blood-stage development","volume":"9","author":"Painter","year":"2018","journal-title":"Nature Communications"},{"key":"bib107","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00267-21","type":"journal-article","article-title":"Inhibitory compounds targeting Plasmodium falciparum gyrase B","volume":"65","author":"Pakosz","year":"2021","journal-title":"Antimicrobial Agents and Chemotherapy"},{"key":"bib108","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1146\/annurev.genet.38.072902.091347","type":"journal-article","article-title":"rRNA transcription in Escherichia coli","volume":"38","author":"Paul","year":"2004","journal-title":"Annual Review of Genetics"},{"key":"bib109","doi-asserted-by":"publisher","first-page":"314","DOI":"10.1111\/j.1365-2958.2006.05443.x","type":"journal-article","article-title":"A role for falcilysin in transit peptide degradation in the Plasmodium falciparum apicoplast","volume":"63","author":"Ponpuak","year":"2007","journal-title":"Molecular Microbiology"},{"key":"bib110","doi-asserted-by":"publisher","first-page":"W200","DOI":"10.1093\/nar\/gky448","type":"journal-article","article-title":"HMMER web server: 2018 update","volume":"46","author":"Potter","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"bib111","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0073783","type":"journal-article","article-title":"Inducible knockdown of Plasmodium gene expression using the glmS ribozyme","volume":"8","author":"Prommana","year":"2013","journal-title":"PLOS ONE"},{"key":"bib112","doi-asserted-by":"publisher","first-page":"7037","DOI":"10.1093\/nar\/gkq565","type":"journal-article","article-title":"Single-stranded DNA binding protein from human malarial parasite Plasmodium falciparum is encoded in the nucleus and targeted to the apicoplast","volume":"38","author":"Prusty","year":"2010","journal-title":"Nucleic Acids Research"},{"key":"bib113","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.molbiopara.2007.04.001","type":"journal-article","article-title":"Nuclear gyrB encodes a functional subunit of the Plasmodium falciparum gyrase that is involved in apicoplast DNA replication","volume":"154","author":"Raghu Ram","year":"2007","journal-title":"Molecular and Biochemical Parasitology"},{"key":"bib114","doi-asserted-by":"publisher","first-page":"5061","DOI":"10.1093\/nar\/gkn483","type":"journal-article","article-title":"DNA organization by the apicoplast-targeted bacterial histone-like protein of Plasmodium falciparum","volume":"36","author":"Ram","year":"2008","journal-title":"Nucleic Acids Research"},{"key":"bib115","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2022.111067","type":"journal-article","article-title":"Resolution of R-loops by topoisomerase III-\u03b2 (TOP3B) in coordination with the DEAD-box helicase DDX5","volume":"40","author":"Saha","year":"2022","journal-title":"Cell Reports"},{"key":"bib116","doi-asserted-by":"publisher","first-page":"1097","DOI":"10.1111\/j.1529-8817.2008.00559.x","type":"journal-article","article-title":"A hypothesis for plastid evolution in chromalveolates(1)","volume":"44","author":"Sanchez-Puerta","year":"2008","journal-title":"Journal of Phycology"},{"key":"bib117","doi-asserted-by":"publisher","first-page":"594","DOI":"10.1038\/s41594-023-00947-3","type":"journal-article","article-title":"A co-transcriptional ribosome assembly checkpoint controls nascent large ribosomal subunit maturation","volume":"30","author":"Sanghai","year":"2023","journal-title":"Nature Structural & Molecular Biology"},{"key":"bib118","doi-asserted-by":"publisher","first-page":"1446","DOI":"10.1016\/j.febslet.2009.03.071","type":"journal-article","article-title":"The Plasmodium HU homolog, which binds the plastid DNA sequence-independent manner, is essential for the parasite\u2019s survival","volume":"583","author":"Sasaki","year":"2009","journal-title":"FEBS Letters"},{"key":"bib119","doi-asserted-by":"publisher","first-page":"1889","DOI":"10.1242\/jcs.112.12.1889","type":"journal-article","article-title":"The nucleolar antigen Nop52, the human homologue of the yeast ribosomal RNA processing RRP1, is recruited at late stages of nucleologenesis","volume":"112","author":"Savino","year":"1999","journal-title":"Journal of Cell Science"},{"key":"bib120","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11112135","type":"journal-article","article-title":"Identification of heme oxygenase-1 as a putative DNa-binding protein","volume":"11","author":"Scaffa","year":"2022","journal-title":"Antioxidants"},{"key":"bib121","doi-asserted-by":"publisher","first-page":"1101","DOI":"10.1038\/nprot.2008.73","type":"journal-article","article-title":"Analyzing real-time PCR data by the comparative C(T) method","volume":"3","author":"Schmittgen","year":"2008","journal-title":"Nature Protocols"},{"key":"bib122","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1007\/978-1-62703-646-7_6","type":"journal-article","article-title":"Clustal Omega, accurate alignment of very large numbers of sequences","volume":"1079","author":"Sievers","year":"2014","journal-title":"Methods in Molecular Biology"},{"key":"bib123","doi-asserted-by":"publisher","first-page":"37793","DOI":"10.1074\/jbc.M112.414078","type":"journal-article","article-title":"Direct tests of enzymatic heme degradation by the malaria parasite Plasmodium falciparum","volume":"287","author":"Sigala","year":"2012","journal-title":"Journal of Biological Chemistry"},{"key":"bib124","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.09143","type":"journal-article","article-title":"Deconvoluting heme biosynthesis to target blood-stage malaria parasites","volume":"4","author":"Sigala","year":"2015","journal-title":"eLife"},{"key":"bib125","doi-asserted-by":"publisher","first-page":"1370","DOI":"10.1101\/gad.300624.117","type":"journal-article","article-title":"SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability","volume":"31","author":"Song","year":"2017","journal-title":"Genes & Development"},{"key":"bib126","doi-asserted-by":"publisher","DOI":"10.1111\/cmi.12719","type":"journal-article","article-title":"The chaperonin TRiC forms an oligomeric complex in the malaria parasite cytosol","volume":"19","author":"Spillman","year":"2017","journal-title":"Cellular Microbiology"},{"key":"bib127","doi-asserted-by":"publisher","first-page":"7293","DOI":"10.1021\/bi025662a","type":"journal-article","article-title":"Crystal structure of rat apo-heme oxygenase-1 (HO-1): mechanism of heme binding in HO-1 inferred from structural comparison of the apo and heme complex forms ,","volume":"41","author":"Sugishima","year":"2002","journal-title":"Biochemistry"},{"key":"bib128","doi-asserted-by":"publisher","first-page":"4517","DOI":"10.1111\/j.1432-1033.2004.04411.x","type":"journal-article","article-title":"Crystal structure of heme oxygenase-1 from cyanobacterium Synechocystis sp. PCC 6803 in complex with heme","volume":"271","author":"Sugishima","year":"2004","journal-title":"European Journal of Biochemistry"},{"key":"bib129","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2020.100062","type":"journal-article","article-title":"An optimized protocol for ChIP-Seq from human embryonic stem cell cultures","volume":"1","author":"Sullivan","year":"2020","journal-title":"STAR Protocols"},{"key":"bib130","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.84491","type":"journal-article","article-title":"The Plasmodium falciparum apicoplast cysteine desulfurase provides sulfur for both iron-sulfur cluster assembly and tRNA modification","volume":"12","author":"Swift","year":"2023","journal-title":"eLife"},{"key":"bib131","doi-asserted-by":"publisher","first-page":"748","DOI":"10.1073\/pnas.61.2.748","type":"journal-article","article-title":"The enzymatic conversion of heme to bilirubin by microsomal heme oxygenase","volume":"61","author":"Tenhunen","year":"1968","journal-title":"PNAS"},{"key":"bib132","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA120.016271","type":"journal-article","article-title":"Crystal structure of higher plant heme oxygenase-1 and its mechanism of interaction with ferredoxin","volume":"296","author":"Tohda","year":"2021","journal-title":"The Journal of Biological Chemistry"},{"key":"bib133","doi-asserted-by":"publisher","DOI":"10.1038\/srep33671","type":"journal-article","article-title":"Structural studies of the yeast DNA damage-inducible protein Ddi1 reveal domain architecture of this eukaryotic protein family","volume":"6","author":"Trempe","year":"2016","journal-title":"Scientific Reports"},{"key":"bib134","doi-asserted-by":"publisher","first-page":"23612","DOI":"10.1074\/jbc.M201748200","type":"journal-article","article-title":"Processing of an apicoplast leader sequence in Plasmodium falciparum and the identification of a putative leader cleavage enzyme","volume":"277","author":"van Dooren","year":"2002","journal-title":"The Journal of Biological Chemistry"},{"key":"bib135","doi-asserted-by":"publisher","first-page":"69075","DOI":"10.18632\/oncotarget.11923","type":"journal-article","article-title":"The non-canonical functions of the heme oxygenases","volume":"7","author":"Vanella","year":"2016","journal-title":"Oncotarget"},{"key":"bib136","doi-asserted-by":"publisher","first-page":"159","DOI":"10.1016\/j.bcp.2018.02.010","type":"journal-article","article-title":"The macrophage heme-heme oxygenase-1 system and its role in inflammation","volume":"153","author":"Vijayan","year":"2018","journal-title":"Biochemical Pharmacology"},{"key":"bib137","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1128\/jb.162.1.458-460.1985","type":"journal-article","article-title":"Effect of DNA gyrase inactivation on RNA synthesis in Escherichia coli","volume":"162","author":"Wahle","year":"1985","journal-title":"Journal of Bacteriology"},{"key":"bib138","doi-asserted-by":"publisher","first-page":"1794","DOI":"10.1093\/emboj\/19.8.1794","type":"journal-article","article-title":"Protein trafficking to the plastid of Plasmodium falciparum is via the secretory pathway","volume":"19","author":"Waller","year":"2000","journal-title":"The EMBO Journal"},{"key":"bib139","doi-asserted-by":"publisher","first-page":"20069","DOI":"10.1074\/jbc.M300989200","type":"journal-article","article-title":"The binding sites on human heme oxygenase-1 for cytochrome P450 Reductase and Biliverdin Reductase","volume":"278","author":"Wang","year":"2003","journal-title":"Journal of Biological Chemistry"},{"key":"bib140","doi-asserted-by":"publisher","first-page":"42593","DOI":"10.1074\/jbc.M406346200","type":"journal-article","article-title":"Human heme oxygenase oxidation of 5- and 15-phenylhemes","volume":"279","author":"Wang","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"bib141","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.360","type":"journal-article","article-title":"Chromatin Immunoprecipitation in Chloroplasts","volume":"2","author":"Wang","year":"2022","journal-title":"Current Protocols"},{"key":"bib142","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.1002\/2211-5463.13453","type":"journal-article","article-title":"Enzymological and structural characterization of Arabidopsis thaliana heme oxygenase\u20101","volume":"12","author":"Wang","year":"2022","journal-title":"FEBS Open Bio"},{"key":"bib143","doi-asserted-by":"publisher","first-page":"1189","DOI":"10.1093\/bioinformatics\/btp033","type":"journal-article","article-title":"Jalview Version 2--a multiple sequence alignment editor and analysis workbench","volume":"25","author":"Waterhouse","year":"2009","journal-title":"Bioinformatics"},{"key":"bib144","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1038\/345253a0","type":"journal-article","article-title":"Chloroquine resistance not linked to mdr-like genes in a Plasmodium falciparum cross","volume":"345","author":"Wellems","year":"1990","journal-title":"Nature"},{"key":"bib145","doi-asserted-by":"publisher","first-page":"603","DOI":"10.1089\/15230860260220102","type":"journal-article","article-title":"Heme oxygenase: evolution, structure, and mechanism","volume":"4","author":"Wilks","year":"2002","journal-title":"Antioxidants & Redox Signaling"},{"key":"bib146","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1038\/nature02362","type":"journal-article","article-title":"Control of gene expression by a natural metabolite-responsive ribozyme","volume":"428","author":"Winkler","year":"2004","journal-title":"Nature"},{"key":"bib147","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1107\/S0907444910045749","type":"journal-article","article-title":"Overview of the CCP4 suite and current developments","volume":"67","author":"Winn","year":"2011","journal-title":"Acta Crystallographica. Section D, Biological Crystallography"},{"key":"bib148","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1007\/978-1-59745-566-4_3","type":"journal-article","article-title":"Identification of SUMO-conjugated proteins and their SUMO attachment sites using proteomic mass spectrometry","volume":"497","author":"Wohlschlegel","year":"2009","journal-title":"Methods in Molecular Biology"},{"key":"bib149","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.06974","type":"journal-article","article-title":"Chromerid genomes reveal the evolutionary path from photosynthetic algae to obligate intracellular parasites","volume":"4","author":"Woo","year":"2015","journal-title":"eLife"},{"key":"bib150","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2021.102170","type":"journal-article","article-title":"The non-canonical effects of heme oxygenase-1, a classical fighter against oxidative stress","volume":"47","author":"Wu","year":"2021","journal-title":"Redox Biology"},{"key":"bib151","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001138","type":"journal-article","article-title":"Chemical rescue of malaria parasites lacking an apicoplast defines organelle function in blood-stage Plasmodium falciparum","volume":"9","author":"Yeh","year":"2011","journal-title":"PLOS Biology"},{"key":"bib152","doi-asserted-by":"publisher","DOI":"10.1126\/science.aap7847","type":"journal-article","article-title":"Uncovering the essential genes of the human malaria parasite Plasmodium falciparum by saturation mutagenesis","volume":"360","author":"Zhang","year":"2018","journal-title":"Science"},{"key":"bib153","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/S0378-1119(01)00776-4","type":"journal-article","article-title":"Deciphering apicoplast targeting signals \u2013 feature extraction from nuclear-encoded precursors of Plasmodium falciparum apicoplast proteins","volume":"280","author":"Zuegge","year":"2001","journal-title":"Gene"},{"key":"dataset1","type":"dataset","article-title":"Crystal structure of PfHO from Plasmodium falciparum at 2.78 A","volume-title":"RCSB Protein Data Bank","author":"Caaveiro","year":"2024","unstructured":"Caaveiro JMM, Senoo A. 2024. Crystal structure of PfHO from Plasmodium falciparum at 2.78 A. RCSB Protein Data Bank. https:\/\/www.rcsb.org\/structure\/8ZLD. 8ZLD."},{"key":"dataset2","doi-asserted-by":"publisher","DOI":"10.25345\/C50K26P12","type":"dataset","article-title":"Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis","volume-title":"MassIVE","author":"Wohlschlegel","year":"2024","unstructured":"Wohlschlegel JA. 2024. Malaria parasites require a divergent heme oxygenase for apicoplast gene expression and biogenesis. MassIVE."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/100256\/elife-100256-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/100256\/elife-100256-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/100256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:02:37Z","timestamp":1787565757000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/100256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,11]]},"references-count":155,"alternative-id":["10.7554\/eLife.100256"],"URL":"https:\/\/doi.org\/10.7554\/elife.100256","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.05.30.596652","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.100256.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.100256.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.100256.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"accession","id":"8ZLD","asserted-by":"subject"},{"id-type":"doi","id":"10.25345\/C50K26P12","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,11]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP100256"}}