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In RPC-specific\n                    <jats:italic>Phosphatase and tensin homolog<\/jats:italic>\n                    conditional knockout (\n                    <jats:italic>Pten-<\/jats:italic>\n                    cKO) and RPC-specific conditional gain-of-function of dominant active PFKFB3 (cytoPFKFB3) mice, glycolytic gene expression and activity are elevated, correlating with precocious rod photoreceptor differentiation and outer segment (OS) maturation. Conversely, glycolytic inhibition in retinal explants suppresses RPC proliferation and photoreceptor differentiation, achieved either with 2-deoxy-\n                    <jats:sc>D<\/jats:sc>\n                    -glucose, a competitive inhibitor of glucose metabolism, by lowering media pH, which disables PKM2, a rate-limiting enzyme, or by inhibiting lactate\/H\n                    <jats:sup>+<\/jats:sup>\n                    symporters, which lowers intracellular pH. Mechanistically, we show that Wnt signaling, the top-upregulated pathway in\n                    <jats:italic>Pten-<\/jats:italic>\n                    cKO retinas, is a glycolysis-dependent pathway. Pharmacological and genetic perturbation of Wnt signaling by knocking-out\n                    <jats:italic>Ctnnb1<\/jats:italic>\n                    , encoding \u03b2-catenin, phenocopies glycolytic inhibition, suppressing RPC proliferation, photoreceptor differentiation, and OS maturation. Thus, developmental rewiring of glycolytic flux modulates Wnt signaling to drive rod photoreceptor differentiation and maturation, an instructive role that may be exploited therapeutically for cell replacement strategies.\n                  <\/jats:p>","DOI":"10.7554\/elife.100604","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T13:25:15Z","timestamp":1728048315000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.100604","source":"Crossref","is-referenced-by-count":1,"title":["Glycolytic flux controls retinal progenitor cell differentiation via regulating Wnt signaling"],"prefix":"10.7554","volume":"13","author":[{"given":"Joseph","family":"Hanna","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05n0tzs53","id-type":"ROR","asserted-by":"publisher"}],"name":"Biological Sciences, Sunnybrook Research Institute","place":["Toronto, 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