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Biofilms also form in the course of many infections.\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    infections frequently involve biofilms, which contribute materially to the difficulty to treat these infections with antibiotic therapy. Many biofilm-related characteristics are controlled by the second messenger, cyclic-di-GMP, which is upregulated on surface contact. Among these factors is the exopolysaccharide Psl, which is a critically important component of the biofilm matrix. Here, we describe the discovery of a\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    bacteriophage, which we have called Clew-1, that directly binds to and uses Psl as a receptor. While this phage does not efficiently infect planktonically growing bacteria, it can disrupt\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    biofilms and replicate in biofilm bacteria. We further demonstrate that the Clew-1 can reduce the bacterial burden in a mouse model of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    keratitis, which is characterized by the formation of a biofilm on the cornea. Due to its reliance on Psl for infection, Clew-1 does not actually form plaques on wild-type bacteria under standard in vitro conditions. This argues that our standard isolation procedures likely exclude bacteriophage that are adapted to using biofilm markers for infection. Importantly, the manner in which we isolated Clew-1 can be easily extended to other strains of\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    and indeed other bacterial species, which will fuel the discovery of other biofilm-tropic bacteriophage and expand their therapeutic use.\n                  <\/jats:p>","DOI":"10.7554\/elife.102352","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T06:25:34Z","timestamp":1731047134000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.102352","source":"Crossref","is-referenced-by-count":4,"title":["A biofilm-tropic Pseudomonas aeruginosa bacteriophage uses the exopolysaccharide Psl as receptor"],"prefix":"10.7554","volume":"13","author":[{"given":"Brenna","family":"Walton","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/051fd9666","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Microbiology, Case Western Reserve 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