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Here, we investigate how the microenvironment of the injured cerebellum changes upon injury and contributes to the regenerative potential of normally gliogenic-NEPs and their adaptive reprogramming. Single-cell transcriptomic and bulk chromatin accessibility analyses of the NEPs from injured neonatal cerebella compared to controls show a temporary increase in cellular processes involved in responding to reactive oxygen species (ROS), a known damage-associated molecular pattern. Analysis of ROS levels in cerebellar tissue confirms a transient increase 1 day after injury at postnatal day 1, overlapping with the peak cell death in the cerebellum. In a transgenic mouse line that ubiquitously overexpresses human mitochondrial catalase (mCAT), ROS is reduced 1 day after injury to the granule cell progenitors, and we demonstrate that several steps in the regenerative process of NEPs are curtailed, leading to reduced cerebellar growth. We also provide preliminary evidence that microglia are involved in one step of adaptive reprogramming by regulating NEP replenishment of the granule cell precursors. Collectively, our results highlight that changes in the tissue microenvironment regulate multiple steps in adaptive reprogramming of NEPs upon death of cerebellar granule cell progenitors at birth, highlighting the instructive roles of microenvironmental signals during regeneration of the neonatal brain.<\/jats:p>","DOI":"10.7554\/elife.102515","type":"journal-article","created":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T11:25:10Z","timestamp":1735903510000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.102515","source":"Crossref","is-referenced-by-count":5,"title":["An increase in reactive oxygen species underlies neonatal cerebellum repair"],"prefix":"10.7554","volume":"14","author":[{"given":"Anna","family":"Pakula","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02yrq0923","id-type":"ROR","asserted-by":"publisher"}],"name":"Developmental Biology Program, Sloan Kettering Institute","place":["New York, United 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