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synthesis are common. Pathways and mechanisms to minimize errors are classified as pre-ribosomal or ribosomal. Pre-ribosomal pathways are primarily concerned with the appropriate charging of tRNAs with their cognate amino acids. By contrast, the ribosomal decoding center is considered \u2018blind\u2019 to mischarged tRNAs since these have cognate codon\u2022anti-codon pairing. Here, we identified that in mycobacteria, deletion of the 16S ribosomal RNA methyltransferase\n                    <jats:italic>gidB<\/jats:italic>\n                    led to increased ribosomal discrimination of mischarged tRNAs. Discrimination only occurred in mycobacteria enriched from environments or genetic backgrounds with high rates of mistranslation. GidB deletion was necessary, but not sufficient for reducing mistranslation due to misacylation. Analysis of new cryo-EM structures of the\n                    <jats:italic>M. smegmatis<\/jats:italic>\n                    ribosomes derived from wild-type and\n                    <jats:italic>gidB<\/jats:italic>\n                    -deleted strains point to the interaction between the base methylated by GidB on the 16S RNA and an asparagine on the ribosomal S12 protein that, when mistranslated to aspartate, may be involved in altering translational fidelity. Our data suggest a mechanism by which mycobacterial ribosomes can discriminate mischarged tRNAs and that 16S rRNA differential methylation by GidB may act to prevent catastrophic translational error.\n                  <\/jats:p>","DOI":"10.7554\/elife.102752","type":"journal-article","created":{"date-parts":[[2024,12,3]],"date-time":"2024-12-03T11:26:04Z","timestamp":1733225164000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.102752","source":"Crossref","is-referenced-by-count":0,"title":["Ribosomal RNA methylation by GidB modulates discrimination of mischarged tRNA"],"prefix":"10.7554","volume":"13","author":[{"given":"Zhuo","family":"Bi","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03cve4549","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Global Health and Infectious Disease, Tsinghua University School of Medicine","place":["Beijing, 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