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While it is clearly known that intracellular\n                    <jats:italic>Mtb<\/jats:italic>\n                    utilize host-derived lipids to maintain infection, the role of macrophage lipid processing on the bacteria\u2019s ability to access the intracellular lipid pool remains undefined. We utilized a CRISPR-Cas9 genetic approach to assess the impact of sequential steps in fatty acid metabolism on the growth of intracellular\n                    <jats:italic>Mtb<\/jats:italic>\n                    . Our analyses demonstrate that macrophages that cannot either import, store, or catabolize fatty acids restrict\n                    <jats:italic>Mtb<\/jats:italic>\n                    growth by both common and divergent antimicrobial mechanisms, including increased glycolysis, increased oxidative stress, production of pro-inflammatory cytokines, enhanced autophagy, and nutrient limitation. We also show that impaired macrophage lipid droplet biogenesis is restrictive to\n                    <jats:italic>Mtb<\/jats:italic>\n                    replication, but increased induction of the same fails to rescue\n                    <jats:italic>Mtb<\/jats:italic>\n                    growth. Our work expands our understanding of how host fatty acid homeostasis impacts\n                    <jats:italic>Mtb<\/jats:italic>\n                    growth in the macrophage.\n                  <\/jats:p>","DOI":"10.7554\/elife.102980","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T05:25:22Z","timestamp":1734326722000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.102980","source":"Crossref","is-referenced-by-count":14,"title":["Impaired fatty acid import or catabolism in macrophages restricts intracellular growth of Mycobacterium tuberculosis"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4734-0518","authenticated-orcid":true,"given":"Nelson V","family":"Simwela","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05bnh6r87","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell 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