{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:34:25Z","timestamp":1787567665564,"version":"build-2736575974"},"reference-count":57,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,7]],"date-time":"2025-07-07T00:00:00Z","timestamp":1751846400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 EY030071"],"award-info":[{"award-number":["R01 EY030071"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008065","name":"Georgia Research Alliance","doi-asserted-by":"publisher","award":["Endowed chair"],"award-info":[{"award-number":["Endowed chair"]}],"id":[{"id":"10.13039\/100008065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Trabecular meshwork (TM) cell therapy has been proposed as a next-generation treatment for elevated intraocular pressure (IOP) in glaucoma, the most common cause of irreversible blindness. Using a magnetic cell steering technique with excellent efficiency and tissue-specific targeting, we delivered two types of cells into a mouse model of glaucoma: either human adipose-derived mesenchymal stem cells (hAMSCs) or induced pluripotent cell derivatives (iPSC-TM cells). We observed a 4.5 [3.1, 6.0] mmHg or 27% reduction in intraocular pressure (IOP) for 9 months after a single dose of only 1500 magnetically steered hAMSCs, explained by increased outflow through the conventional pathway and associated with a higher TM cellularity. iPSC-TM cells were also effective, but less so, showing only a 1.9 [0.4, 3.3] mmHg or 13% IOP reduction and increased risk of tumorigenicity. In both cases, injected cells remained detectable in the iridocorneal angle 3 weeks post-transplantation. Based on the locations of the delivered cells, the mechanism of IOP lowering is most likely paracrine signaling. We conclude that magnetically steered hAMSC cell therapy has potential for long-term treatment of ocular hypertension in glaucoma.<\/jats:p>","DOI":"10.7554\/elife.103256","type":"journal-article","created":{"date-parts":[[2024,11,28]],"date-time":"2024-11-28T12:25:30Z","timestamp":1732796730000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.103256","source":"Crossref","is-referenced-by-count":5,"title":["Magnetically steered cell therapy for reduction of intraocular pressure as a treatment strategy for open-angle glaucoma"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-1369-8772","authenticated-orcid":true,"given":"M Reza","family":"Bahranifard","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Woodruff School of Mechanical Engineering, Georgia Institute of Technology","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Jessica","family":"Chan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Chemistry and Biochemistry, Georgia Institute of Technology","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2764-6413","authenticated-orcid":true,"given":"A Thomas","family":"Read","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology\/Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-4047-8546","authenticated-orcid":true,"given":"Guorong","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ophthalmology, Duke University","place":["Durham, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Lin","family":"Cheng","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ophthalmology and Visual Sciences, The University of Iowa","place":["Iowa City, United States"]},{"id":[{"id":"https:\/\/ror.org\/04hgm3062","id-type":"ROR","asserted-by":"publisher"}],"name":"Veterans Administration Center for the Prevention and Treatment of Visual Loss, Iowa City VA Healthcare System","place":["Iowa City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1849-2870","authenticated-orcid":true,"given":"Babak N","family":"Safa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology\/Emory University","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/032db5x82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medical Engineering, University of South Florida","place":["Tampa, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5063-0305","authenticated-orcid":true,"given":"Seyed Mohammad","family":"Siadat","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology\/Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7126-4463","authenticated-orcid":true,"given":"Anamik","family":"Jhunjhunwala","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology\/Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Hans E","family":"Grossniklaus","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03czfpz43","id-type":"ROR","asserted-by":"publisher"}],"name":"Departments of Ophthalmology and Pathology, Emory University School of Medicine","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Stanislav Y","family":"Emelianov","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology\/Emory University","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"School of Electrical and Computer Engineering, Georgia Institute of Technology","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2504-8997","authenticated-orcid":true,"given":"W Daniel","family":"Stamer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ophthalmology, Duke University","place":["Durham, United States"]},{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biomedical Engineering, Duke University","place":["Durham, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Markus H","family":"Kuehn","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/036jqmy94","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ophthalmology and Visual Sciences, The University of Iowa","place":["Iowa City, United States"]},{"id":[{"id":"https:\/\/ror.org\/04hgm3062","id-type":"ROR","asserted-by":"publisher"}],"name":"Veterans Administration Center for the Prevention and Treatment of Visual Loss, Iowa City VA Healthcare System","place":["Iowa City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6110-3052","authenticated-orcid":true,"given":"C Ross","family":"Ethier","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Woodruff School of Mechanical Engineering, Georgia Institute of Technology","place":["Atlanta, United States"]},{"id":[{"id":"https:\/\/ror.org\/01zkghx44","id-type":"ROR","asserted-by":"publisher"}],"name":"Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology\/Emory University","place":["Atlanta, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2025,7,7]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"751","DOI":"10.1002\/stem.1885","type":"journal-article","article-title":"Induced pluripotent stem cells restore function in a human cell loss model of open-angle glaucoma","volume":"33","author":"Abu-Hassan","year":"2015","journal-title":"Stem Cells"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0002-9394(89)90805-2","type":"journal-article","article-title":"Trabecular repopulation by anterior trabecular meshwork cells after laser trabeculoplasty","volume":"107","author":"Acott","year":"1989","journal-title":"American Journal of Ophthalmology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1016\/s0161-6420(84)34248-8","type":"journal-article","article-title":"Trabecular meshwork cellularity in primary open-angle glaucoma and nonglaucomatous normals","volume":"91","author":"Alvarado","year":"1984","journal-title":"Ophthalmology"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2023.109602","type":"journal-article","article-title":"Improved magnetic delivery of cells to the trabecular meshwork in mice","volume":"234","author":"Bahrani Fard","year":"2023","journal-title":"Experimental Eye Research"},{"key":"bib5","first-page":"1734","type":"journal-article","article-title":"Quantitative tumorigenicity assays using the anterior chamber of the mouse eye","volume":"28","author":"Boone","year":"1968","journal-title":"Cancer Research"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"7499","DOI":"10.1167\/iovs.14-14872","type":"journal-article","article-title":"Identification of adult stem cells in Schwalbe\u2019s line region of the primate eye","volume":"55","author":"Braunger","year":"2014","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"633","DOI":"10.1016\/j.exer.2003.07.002","type":"journal-article","article-title":"Goldmann\u2019s equation and clinical measures of aqueous dynamics","volume":"78","author":"Brubaker","year":"2004","journal-title":"Experimental Eye Research"},{"key":"bib8","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00143","type":"journal-article","article-title":"Aggregation of human mesenchymal stromal cells eliminates their ability to suppress human T cells","volume":"11","author":"Burand","year":"2020","journal-title":"Frontiers in Immunology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"S3","DOI":"10.1016\/j.survophthal.2008.08.006","type":"journal-article","article-title":"Risk factors for glaucoma onset and progression","volume":"53","author":"Coleman","year":"2008","journal-title":"Survey of Ophthalmology"},{"key":"bib10","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2022.101063","type":"journal-article","article-title":"A novel glaucoma approach: Stem cell regeneration of the trabecular meshwork","volume":"90","author":"Coulon","year":"2022","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"7065","DOI":"10.1167\/iovs.14-14800","type":"journal-article","article-title":"Induction of trabecular meshwork cells from induced pluripotent stem cells","volume":"55","author":"Ding","year":"2014","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib12","first-page":"1741","type":"journal-article","article-title":"Calculations of flow resistance in the juxtacanalicular meshwork","volume":"27","author":"Ethier","year":"1986","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib13","doi-asserted-by":"publisher","DOI":"10.20411\/pai.v2i3.186","type":"journal-article","article-title":"The critical role of nonhuman primates in medical research - white paper","volume":"2","author":"Friedman","year":"2017","journal-title":"Pathogens and Immunity"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1016\/s0014-4835(03)00220-3","type":"journal-article","article-title":"The effect of TGF-beta2 on human trabecular meshwork extracellular proteolytic system","volume":"77","author":"Fuchshofer","year":"2003","journal-title":"Experimental Eye Research"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"1326","DOI":"10.1093\/gerona\/glt032","type":"journal-article","article-title":"C57BL\/6 neuromuscular healthspan scoring system","volume":"68","author":"Graber","year":"2013","journal-title":"The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.1001\/archopht.120.10.1268","type":"journal-article","article-title":"Reduction of intraocular pressure and glaucoma progression: results from the Early Manifest Glaucoma Trial","volume":"120","author":"Heijl","year":"2002","journal-title":"Archives of Ophthalmology"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1093\/stcltm\/szac036","type":"journal-article","article-title":"The Anterior eye chamber as a visible medium for in vivo tumorigenicity tests","volume":"11","author":"Inagaki","year":"2022","journal-title":"Stem Cells Translational Medicine"},{"key":"bib18","first-page":"249","type":"journal-article","article-title":"Intraocular pressure in inbred mouse strains","volume":"38","author":"John","year":"1997","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047559","type":"journal-article","article-title":"Safety of cell therapy with mesenchymal stromal cells (SafeCell): a systematic review and meta-analysis of clinical trials","volume":"7","author":"Lalu","year":"2012","journal-title":"PLOS ONE"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/j.stem.2015.11.003","type":"journal-article","article-title":"Genomic instability in human pluripotent stem cells arises from replicative stress and chromosome condensation defects","volume":"18","author":"Lamm","year":"2016","journal-title":"Cell Stem Cell"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"1714","DOI":"10.1073\/pnas.1814889116","type":"journal-article","article-title":"In vivo measurement of trabecular meshwork stiffness in a corticosteroid-induced ocular hypertensive mouse model","volume":"116","author":"Li","year":"2019","journal-title":"PNAS"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.60831","type":"journal-article","article-title":"Anti-fibrotic activity of a rho-kinase inhibitor restores outflow function and intraocular pressure homeostasis","volume":"10","author":"Li","year":"2021","journal-title":"eLife"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"1136","DOI":"10.1002\/stem.1364","type":"journal-article","article-title":"Transplantation of mesenchymal stem cells promotes tissue regeneration in a glaucoma model through laser-induced paracrine factor secretion and progenitor cell recruitment","volume":"31","author":"Manuguerra-Gagn\u00e9","year":"2013","journal-title":"Stem Cells"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.63.2.12","type":"journal-article","article-title":"Consensus recommendation for mouse models of ocular hypertension to study aqueous humor outflow and its mechanisms","volume":"63","author":"McDowell","year":"2022","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1117\/1.JBO.24.10.106502","type":"journal-article","article-title":"Determination of cell nucleus-to-cytoplasmic ratio using imaging flow cytometry and a combined ultrasound and photoacoustic technique: a comparison study","volume":"24","author":"Moore","year":"2019","journal-title":"Journal of Biomedical Optics"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1006\/exer.1996.0184","type":"journal-article","article-title":"A rat model of chronic pressure-induced optic nerve damage","volume":"64","author":"Morrison","year":"1997","journal-title":"Experimental Eye Research"},{"key":"bib27","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20102406","type":"journal-article","article-title":"Genetic stability of mesenchymal stromal cells for regenerative medicine applications: a fundamental biosafety aspect","volume":"20","author":"Neri","year":"2019","journal-title":"International Journal of Molecular Sciences"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"175","DOI":"10.1007\/BF00315125","type":"journal-article","article-title":"Brain tissue transplanted to the anterior chamber of the eye","volume":"135","author":"Olson","year":"1972","journal-title":"Zeitschrift F\u00fcr Zellforschung Und Mikroskopische Anatomie"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"4922","DOI":"10.1167\/iovs.14-14429","type":"journal-article","article-title":"Ultrastructural changes associated with dexamethasone-induced ocular hypertension in mice","volume":"55","author":"Overby","year":"2014","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib30","doi-asserted-by":"publisher","DOI":"10.1098\/rsfs.2013.0001","type":"journal-article","article-title":"From seeing to believing: labelling strategies for in vivo cell-tracking experiments","volume":"3","author":"Progatzky","year":"2013","journal-title":"Interface Focus"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"365","DOI":"10.3109\/02713683.2013.845224","type":"journal-article","article-title":"Effects of three commonly used anesthetics on intraocular pressure in mouse","volume":"39","author":"Qiu","year":"2014","journal-title":"Current Eye Research"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"444","DOI":"10.1016\/j.actbio.2018.02.037","type":"journal-article","article-title":"Glaucomatous cell derived matrices differentially modulate non-glaucomatous trabecular meshwork cellular behavior","volume":"71","author":"Raghunathan","year":"2018","journal-title":"Acta Biomaterialia"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"441","DOI":"10.2147\/PPA.S23780","type":"journal-article","article-title":"Objective assessment of compliance and persistence among patients treated for glaucoma and ocular hypertension: a systematic review","volume":"5","author":"Reardon","year":"2011","journal-title":"Patient Preference and Adherence"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1016\/j.arcmed.2020.08.006","type":"journal-article","article-title":"Mesenchymal stem cells current clinical applications: a systematic review","volume":"52","author":"Rodr\u00edguez-Fuentes","year":"2021","journal-title":"Archives of Medical Research"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1186\/s13287-015-0168-0","type":"journal-article","article-title":"Intraocular pressure reduction and neuroprotection conferred by bone marrow-derived mesenchymal stem cells in an animal model of glaucoma","volume":"6","author":"Roubeix","year":"2015","journal-title":"Stem Cell Research & Therapy"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"466","DOI":"10.1016\/j.jmmm.2004.11.131","type":"journal-article","article-title":"Genotoxicity and inflammatory investigation in mice treated with magnetite nanoparticles surface coated with polyaspartic acid","volume":"289","author":"Sadeghiani","year":"2005","journal-title":"Journal of Magnetism and Magnetic Materials"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1167\/iovs.09-3947","type":"journal-article","article-title":"The microbead occlusion model: a paradigm for induced ocular hypertension in rats and mice","volume":"51","author":"Sappington","year":"2010","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1038\/nmeth.2089","type":"journal-article","article-title":"NIH Image to ImageJ: 25 years of image analysis","volume":"9","author":"Schneider","year":"2012","journal-title":"Nature Methods"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0150694","type":"journal-article","article-title":"Measurement of outflow facility using iperfusion","volume":"11","author":"Sherwood","year":"2016","journal-title":"PLOS ONE"},{"key":"bib40","doi-asserted-by":"publisher","DOI":"10.3402\/nano.v1i0.5358","type":"journal-article","article-title":"Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION)","volume":"1","author":"Singh","year":"2010","journal-title":"Nano Reviews"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"976","DOI":"10.1016\/j.freeradbiomed.2004.01.016","type":"journal-article","article-title":"Iron oxide particles for molecular magnetic resonance imaging cause transient oxidative stress in rat macrophages","volume":"36","author":"Stroh","year":"2004","journal-title":"Free Radical Biology & Medicine"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"S101","DOI":"10.1016\/j.survophthal.2007.08.002","type":"journal-article","article-title":"What increases outflow resistance in primary open-angle glaucoma?","volume":"52","author":"Tamm","year":"2007","journal-title":"Survey of Ophthalmology"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1016\/j.ophtha.2014.05.013","type":"journal-article","article-title":"Global prevalence of glaucoma and projections of glaucoma burden through 2040: a systematic review and meta-analysis","volume":"121","author":"Tham","year":"2014","journal-title":"Ophthalmology"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1006\/exer.1994.1158","type":"journal-article","article-title":"Aqueous humor in glaucomatous eyes contains an increased level of TGF-beta 2","volume":"59","author":"Tripathi","year":"1994","journal-title":"Experimental Eye Research"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2020.108420","type":"journal-article","article-title":"Effect of inhalation anesthesia with isoflurane on circadian rhythm of murine intraocular pressure","volume":"203","author":"Tsuchiya","year":"2021","journal-title":"Experimental Eye Research"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1111\/ceo.12992","type":"journal-article","article-title":"DBA\/2J mouse model for experimental glaucoma: pitfalls and problems","volume":"45","author":"Turner","year":"2017","journal-title":"Clinical & Experimental Ophthalmology"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.2147\/IJN.S346141","type":"journal-article","article-title":"Magnetic nano-platform enhanced ipsc-derived trabecular meshwork delivery and tracking efficiency","volume":"17","author":"Wang","year":"2022","journal-title":"International Journal of Nanomedicine"},{"key":"bib48","first-page":"488","type":"journal-article","article-title":"Neuropathology for the neuroradiologist: rosettes and pseudorosettes","volume":"27","author":"Wippold","year":"2006","journal-title":"AJNR. American Journal of Neuroradiology"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.63677","type":"journal-article","article-title":"Stem cell transplantation rescued a primary open-angle glaucoma mouse model","volume":"10","author":"Xiong","year":"2021","journal-title":"eLife"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1016\/j.stem.2020.09.014","type":"journal-article","article-title":"Pluripotent stem cell-based cell therapy-promise and challenges","volume":"27","author":"Yamanaka","year":"2020","journal-title":"Cell Stem Cell"},{"key":"bib51","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0107446","type":"journal-article","article-title":"A laser-induced mouse model with long-term intraocular pressure elevation","volume":"9","author":"Yun","year":"2014","journal-title":"PLOS ONE"},{"key":"bib52","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0227-z","type":"journal-article","article-title":"Human stem cells home to and repair laser-damaged trabecular meshwork in a mouse model","volume":"1","author":"Yun","year":"2018","journal-title":"Communications Biology"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"7160","DOI":"10.1096\/fj.201902326R","type":"journal-article","article-title":"Adipose-derived stem cells integrate into trabecular meshwork with glaucoma treatment potential","volume":"34","author":"Zhou","year":"2020","journal-title":"FASEB Journal"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"E3492","DOI":"10.1073\/pnas.1604153113","type":"journal-article","article-title":"Transplantation of iPSC-derived TM cells rescues glaucoma phenotypes in vivo","volume":"113","author":"Zhu","year":"2016","journal-title":"PNAS"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"2054","DOI":"10.1167\/iovs.16-20672","type":"journal-article","article-title":"Restoration of Aqueous Humor Outflow Following Transplantation of iPSC-derived trabecular meshwork cells in a transgenic mouse model of glaucoma","volume":"58","author":"Zhu","year":"2017","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib56","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-59941-0","type":"journal-article","article-title":"Transplantation of iPSC-TM stimulates division of trabecular meshwork cells in human eyes","volume":"10","author":"Zhu","year":"2020","journal-title":"Scientific Reports"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"3542","DOI":"10.1172\/JCI58183","type":"journal-article","article-title":"Reduction of ER stress via a chemical chaperone prevents disease phenotypes in a mouse model of primary open angle glaucoma","volume":"121","author":"Zode","year":"2011","journal-title":"The Journal of Clinical Investigation"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/103256\/elife-103256-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/103256\/elife-103256-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/103256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:33:07Z","timestamp":1787563987000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/103256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,7]]},"references-count":57,"alternative-id":["10.7554\/eLife.103256"],"URL":"https:\/\/doi.org\/10.7554\/elife.103256","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.05.13.593917","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2024.05.13.593917","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.103256.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.103256.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.103256.2","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,7]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP103256"}}