{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:34:57Z","timestamp":1787567697862,"version":"build-2736575974"},"reference-count":82,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,7,15]],"date-time":"2025-07-15T00:00:00Z","timestamp":1752537600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM145352"],"award-info":[{"award-number":["R35GM145352"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000060","name":"National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["R03AI178064"],"award-info":[{"award-number":["R03AI178064"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Johns Hopkins University Catalyst Award"}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    During oogenesis, self-renewal and differentiation of germline stem cells (GSCs) must be tightly regulated. The\n                    <jats:italic>Drosophila<\/jats:italic>\n                    female germline serves as an excellent model for studying these regulatory mechanisms. Here, we report that a previously uncharacterized gene\n                    <jats:italic>CG14545<\/jats:italic>\n                    , which we named\n                    <jats:italic>sakura<\/jats:italic>\n                    , is essential for oogenesis and female fertility in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    . Sakura is predominantly expressed in the ovaries, particularly in the germline cells, including GSCs. sakura null mutant female flies display rudimentary ovaries with germline-less and tumorous phenotypes, fail to produce eggs, and are completely sterile. The germline-specific depletion of sakura impairs Dpp\/BMP signaling, leading to aberrant\n                    <jats:italic>bag-of-marbles<\/jats:italic>\n                    (\n                    <jats:italic>bam<\/jats:italic>\n                    ) expression, resulting in faulty differentiation and loss of GSCs. sakura is also necessary for normal levels of piwi-interacting RNAs (piRNAs) levels and for female-specific splicing of\n                    <jats:italic>sex-lethal<\/jats:italic>\n                    (\n                    <jats:italic>sxl<\/jats:italic>\n                    ), a master regulator of sex identity determination. We identified Ovarian Tumor (Otu) as a protein binding partner of Sakura and found that loss of\n                    <jats:italic>otu<\/jats:italic>\n                    phenocopies loss of\n                    <jats:italic>sakura<\/jats:italic>\n                    in ovaries. Thus, we identify Sakura as a crucial factor for GSC renewal and differentiation and oogenesis, and propose that Sakura and Otu function together in these processes.\n                  <\/jats:p>","DOI":"10.7554\/elife.103828","type":"journal-article","created":{"date-parts":[[2024,11,27]],"date-time":"2024-11-27T11:26:21Z","timestamp":1732706781000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.103828","source":"Crossref","is-referenced-by-count":1,"title":["Female-germline specific protein Sakura interacts with Otu and is crucial for germline stem cell renewal and differentiation and oogenesis"],"prefix":"10.7554","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6474-4875","authenticated-orcid":true,"given":"Azali","family":"Azlan","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00za53h95","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cell Biology Johns Hopkins University School of Medicine","place":["Baltimore, United 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