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We provide a comparison of the specificity and kinetics of the PET signals acquired when using nanobodies radiolabeled with\n                    <jats:sup>89<\/jats:sup>\n                    Zr,\n                    <jats:sup>64<\/jats:sup>\n                    Cu, and\n                    <jats:sup>18<\/jats:sup>\n                    F, and find that the chelation of the\n                    <jats:sup>89<\/jats:sup>\n                    Zr and\n                    <jats:sup>64<\/jats:sup>\n                    Cu radioisotopes to anti-TfR nanobodies results in radioisotope release upon endocytosis of the radiolabeled nanobodies. We used a knock-in mouse that expresses a TfR with a human ectodomain (Tfrc\n                    <jats:italic>\n                      <jats:sup>hu\/hu<\/jats:sup>\n                    <\/jats:italic>\n                    ) as a source of bone marrow for transplants into C57BL\/6 recipients and show that V\n                    <jats:sub>H<\/jats:sub>\n                    H188 detects such transplants by PET\/CT. Conversely, C57BL\/6 bone marrow and B16.F10 melanoma cell line transplanted into Tfrc\n                    <jats:italic>\n                      <jats:sup>hu\/hu<\/jats:sup>\n                    <\/jats:italic>\n                    recipients can be imaged with V\n                    <jats:sub>H<\/jats:sub>\n                    H123. In C57BL\/6 mice impregnated by Tfrc\n                    <jats:italic>\n                      <jats:sup>hu\/hu<\/jats:sup>\n                    <\/jats:italic>\n                    males, we saw an intense V\n                    <jats:sub>H<\/jats:sub>\n                    H188 signal in the placenta, showing that TfR-specific V\n                    <jats:sub>H<\/jats:sub>\n                    Hs accumulate at the placental barrier but do not enter the fetal tissue. We were unable to observe accumulation of the anti-TfR radiotracers in the central nervous system (CNS) by PET\/CT but showed evidence of CNS accumulation by radiospectrometry. The model presented here can be used to track many transplanted cell types by PET\/CT, provided cells express TfR, as is typically the case for proliferating cells such as tumor lines.\n                  <\/jats:p>","DOI":"10.7554\/elife.104302","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T05:25:42Z","timestamp":1736227542000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.104302","source":"Crossref","is-referenced-by-count":2,"title":["A pair of congenic mice for imaging of transplants by positron emission tomography using anti-transferrin receptor nanobodies"],"prefix":"10.7554","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0156-8936","authenticated-orcid":true,"given":"Thomas","family":"Balligand","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00dvg7y05","id-type":"ROR","asserted-by":"publisher"}],"name":"Program for Cellular and Molecular Medicine, Boston Children\u2019s Hospital, Harvard Medical 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