{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T21:04:07Z","timestamp":1789506247361,"version":"build-2803163510"},"reference-count":124,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,6,24]],"date-time":"2025-06-24T00:00:00Z","timestamp":1750723200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["T32EY024234"],"award-info":[{"award-number":["T32EY024234"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY022076"],"award-info":[{"award-number":["R01EY022076"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R0EY1031817"],"award-info":[{"award-number":["R0EY1031817"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["P30EY014800"],"award-info":[{"award-number":["P30EY014800"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY034096"],"award-info":[{"award-number":["R01EY034096"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY022359"],"award-info":[{"award-number":["R01EY022359"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000053","name":"National Eye Institute","doi-asserted-by":"publisher","award":["R01EY005722"],"award-info":[{"award-number":["R01EY005722"]}],"id":[{"id":"10.13039\/100000053","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Crandall Glaucoma Initiative"},{"name":"Stauss-Ranking Foundation"},{"DOI":"10.13039\/100001818","name":"Research to Prevent Blindness","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100001818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R21EY036189"],"award-info":[{"award-number":["R21EY036189"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    The risk for developing primary open-angle glaucoma (POAG) correlates with the magnitude of ocular hypertension (OHT) and the concentration of transforming growth factor-\u03b22 (TGF\u03b22) in the aqueous humor. Effective treatment of POAG requires a detailed understanding of the interaction between pressure sensing mechanisms in the trabecular meshwork (TM) and biochemical risk factors. Here, we employed molecular, optical, electrophysiological, and tonometric strategies to establish the role of TGF\u03b22 in transcription and functional expression of mechanosensitive channel isoforms alongside studies of TM contractility in biomimetic hydrogels and intraocular pressure (IOP) regulation in a mouse model of TGF\u03b22-induced OHT. TGF\u03b22 upregulated expression of\n                    <jats:italic>Trpv4<\/jats:italic>\n                    and\n                    <jats:italic>Piezo1<\/jats:italic>\n                    transcripts and time-dependently augmented functional TRPV4 activation. TRPV4 agonists induced contractility of TM-seeded hydrogels, whereas pharmacological inhibition suppressed TGF\u03b22-induced hypercontractility and abrogated OHT in eyes overexpressing TGF\u03b22.\n                    <jats:italic>Trpv4<\/jats:italic>\n                    -deficient mice resisted TGF\u03b22-driven increases in IOP, but nocturnal OHT was not additive to TGF\u03b2-evoked OHT. Our study establishes the fundamental role of TGF\u03b2 as a modulator of mechanosensing in nonexcitable cells, identifies the TRPV4 channel as the final common mechanism for TM contractility and circadian and pathological OHT, and offers insights for future treatments that can lower IOP in the sizeable cohort of hypertensive glaucoma patients that resist current treatments.\n                  <\/jats:p>","DOI":"10.7554\/elife.104894","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T12:25:10Z","timestamp":1737462310000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.104894","source":"Crossref","is-referenced-by-count":11,"title":["TRPV4 activation by TGF\u03b22 enhances cellular contractility and drives ocular hypertension"],"prefix":"10.7554","volume":"14","author":[{"given":"Christopher Nass","family":"Rudzitis","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Salt Lake City, United States"]},{"id":[{"id":"https:\/\/ror.org\/03r0ha626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neurobiology, University of Utah","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"validation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Monika","family":"Lakk","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Ayushi","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Syracuse, United States"]},{"id":[{"id":"https:\/\/ror.org\/040kfrw16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cell and Developmental Biology, SUNY Upstate Medical University","place":["Syracuse, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Sarah N","family":"Redmon","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Denisa","family":"Kirdajov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Yun-Ting","family":"Tseng","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Michael L","family":"De Ieso","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ophthalmology, Duke Eye Center, Duke University","place":["Durham, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"W Daniel","family":"Stamer","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00py81415","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Ophthalmology, Duke Eye Center, Duke University","place":["Durham, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"}]},{"given":"Samuel","family":"Herberg","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Syracuse, United States"]},{"id":[{"id":"https:\/\/ror.org\/040kfrw16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cell and Developmental Biology, SUNY Upstate Medical University","place":["Syracuse, United States"]},{"id":[{"id":"https:\/\/ror.org\/040kfrw16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University","place":["Syracuse, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4468-3029","authenticated-orcid":true,"given":"David","family":"Krizaj","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences","place":["Salt Lake City, United States"]},{"id":[{"id":"https:\/\/ror.org\/03r0ha626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Neurobiology, University of Utah","place":["Salt Lake City, United States"]},{"id":[{"id":"https:\/\/ror.org\/03r0ha626","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Bioengineering, University of Utah","place":["Salt Lake City, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"resources","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2025,6,24]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.07-1080","type":"journal-article","article-title":"Activation of store-operated Ca 2+ channels in trabecular meshwork cells","volume":"49","author":"Abad","year":"2008","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100897","type":"journal-article","article-title":"Normal and glaucomatous outflow regulation","volume":"82","author":"Acott","year":"2021","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/j.yjmcc.2012.10.016","type":"journal-article","article-title":"TRPV4 channels mediate cardiac fibroblast differentiation by integrating mechanical and soluble signals","volume":"54","author":"Adapala","year":"2013","journal-title":"Journal of Molecular and Cellular Cardiology"},{"key":"bib4","first-page":"612","type":"journal-article","article-title":"Aqueous humor TGF-\u03b22 levels in patients with open-angle glaucoma: a meta-analysis","volume":"21","author":"Agarwal","year":"2015","journal-title":"Molecular Vision"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"3864","DOI":"10.1523\/JNEUROSCI.5385-05.2006","type":"journal-article","article-title":"A transient receptor potential vanilloid 4-dependent mechanism of hyperalgesia is engaged by concerted action of inflammatory mediators","volume":"26","author":"Alessandri-Haber","year":"2006","journal-title":"The Journal of Neuroscience"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1016\/j.preteyeres.2011.11.002","type":"journal-article","article-title":"The molecular basis of retinal ganglion cell death in glaucoma","volume":"31","author":"Almasieh","year":"2012","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib7","doi-asserted-by":"publisher","DOI":"10.3389\/fopht.2022.948397","type":"journal-article","article-title":"Effects of netarsudil-family rho kinase inhibitors on human trabecular meshwork cell contractility and actin remodeling using a bioengineered ECM hydrogel","volume":"2","author":"Bagu\u00e9","year":"2022","journal-title":"Frontiers in Ophthalmology"},{"key":"bib8","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100916","type":"journal-article","article-title":"Inflammation in glaucoma: from the back to the front of the eye, and beyond","volume":"83","author":"Baudouin","year":"2021","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"C403","DOI":"10.1152\/ajpcell.00295.2024","type":"journal-article","article-title":"TRPV4 and chloride channels mediate volume sensing in trabecular meshwork cells","volume":"327","author":"Baumann","year":"2024","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/s1350-9462(03)00018-1","type":"journal-article","article-title":"Gene expression in the trabecular meshwork and the influence of intraocular pressure","volume":"22","author":"Borr\u00e1s","year":"2003","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"182","DOI":"10.1001\/jamaophthalmol.2014.4747","type":"journal-article","article-title":"Prevention of nocturnal elevation of intraocular pressure by gene transfer of dominant-negative RhoA in rats","volume":"133","author":"Borr\u00e1s","year":"2015","journal-title":"JAMA Ophthalmology"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"5838","DOI":"10.1167\/iovs.12-9923","type":"journal-article","article-title":"Pharmacologic manipulation of conventional outflow facility in ex vivo mouse eyes","volume":"53","author":"Boussommier-Calleja","year":"2012","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib13","first-page":"286","type":"journal-article","article-title":"The effect of intraocular pressure on conventional outflow resistance in the enucleated human eye","volume":"14","author":"Brubaker","year":"1975","journal-title":"Investigative Ophthalmology"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1097\/CCM.0b013e31822f09d7","type":"journal-article","article-title":"Mechanical stress induces lung fibrosis by epithelial-mesenchymal transition","volume":"40","author":"Cabrera-Ben\u00edtez","year":"2012","journal-title":"Critical Care Medicine"},{"key":"bib15","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-13573-8","type":"journal-article","article-title":"Genome-wide transcriptome profiling of human trabecular meshwork cells treated with TGF-\u03b22","volume":"12","author":"Callaghan","year":"2022","journal-title":"Scientific Reports"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"108","DOI":"10.1016\/j.preteyeres.2016.12.004","type":"journal-article","article-title":"Aqueous outflow - A continuum from trabecular meshwork to episcleral veins","volume":"57","author":"Carreon","year":"2017","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib17","doi-asserted-by":"publisher","DOI":"10.3390\/cells12121654","type":"journal-article","article-title":"Pathophysiological roles of the TRPV4 Channel in the Heart","volume":"12","author":"Chaigne","year":"2023","journal-title":"Cells"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1161\/CIRCULATIONAHA.121.058607","type":"journal-article","article-title":"Novel smooth muscle Ca2+-signaling nanodomains in blood pressure regulation","volume":"146","author":"Chen","year":"2022","journal-title":"Circulation"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1016\/S0923-1811(99)00008-0","type":"journal-article","article-title":"Transforming growth factors-beta 1, -beta 2, and -beta 3 stimulate fibroblast procollagen production in vitro but are differentially expressed during bleomycin-induced lung fibrosis","volume":"150","author":"Coker","year":"1997","journal-title":"The American Journal of Pathology"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.brainresbull.2020.05.010","type":"journal-article","article-title":"Expression and functional characterization of transient receptor potential vanilloid 4 in the dorsal root ganglion and spinal cord of diabetic rats with mechanical allodynia","volume":"162","author":"Cui","year":"2020","journal-title":"Brain Research Bulletin"},{"key":"bib21","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2023130118","type":"journal-article","article-title":"Caveolar peroxynitrite formation impairs endothelial TRPV4 channels and elevates pulmonary arterial pressure in pulmonary hypertension","volume":"118","author":"Daneva","year":"2021","journal-title":"PNAS"},{"key":"bib22","first-page":"424","type":"journal-article","article-title":"Localization of smooth muscle and nonmuscle actin isoforms in the human aqueous outflow pathway","volume":"33","author":"de Kater","year":"1992","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib23","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-0327","type":"journal-article","article-title":"Effects of latrunculin-b on outflow facility and trabecular meshwork structure in human eyes","volume":"47","author":"Ethier","year":"2006","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05226-y","type":"journal-article","article-title":"The production of fibroblast growth factor 23 is controlled by TGF-\u03b22","volume":"7","author":"Feger","year":"2017","journal-title":"Scientific Reports"},{"key":"bib25","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.05-1060","type":"journal-article","article-title":"TGF\u03b22-induced changes in human trabecular meshwork: implications for intraocular pressure","volume":"47","author":"Fleenor","year":"2006","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.04-0649","type":"journal-article","article-title":"Transforming growth factor-\u03b22 modulated extracellular matrix component expression in cultured human optic nerve head astrocytes","volume":"46","author":"Fuchshofer","year":"2005","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1020","DOI":"10.1016\/j.exer.2009.01.002","type":"journal-article","article-title":"Gene expression profiling of TGF\u03b22- and\/or BMP7-treated trabecular meshwork cells: Identification of Smad7 as a critical inhibitor of TGF-\u03b22 signaling","volume":"88","author":"Fuchshofer","year":"2009","journal-title":"Experimental Eye Research"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1007\/s00441-011-1274-7","type":"journal-article","article-title":"The role of TGF-\u03b2 in the pathogenesis of primary open-angle glaucoma","volume":"347","author":"Fuchshofer","year":"2012","journal-title":"Cell and Tissue Research"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1001\/archopht.120.6.714","type":"journal-article","article-title":"The ocular hypertension treatment study: baseline factors that predict the onset of primary open-angle glaucoma","volume":"120","author":"Gordon","year":"2002","journal-title":"Archives of Ophthalmology"},{"key":"bib30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011654","type":"journal-article","article-title":"Importance of non-selective cation channel TRPV4 interaction with cytoskeleton and their reciprocal regulations in cultured cells","volume":"5","author":"Goswami","year":"2010","journal-title":"PLOS ONE"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1167\/iovs.03-0796","type":"journal-article","article-title":"Effects of TGF- 2 in perfused human eyes","volume":"45","author":"Gottanka","year":"2004","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib32","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aau1533","type":"journal-article","article-title":"Translocation of TRPV4-PI3K\u03b3 complexes to the plasma membrane drives myofibroblast transdifferentiation","volume":"12","author":"Grove","year":"2019","journal-title":"Science Signaling"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"6408","DOI":"10.1523\/JNEUROSCI.22-15-06408.2002","type":"journal-article","article-title":"Heat-evoked activation of the ion channel, TRPV4","volume":"22","author":"G\u00fcler","year":"2002","journal-title":"The Journal of Neuroscience"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"1268","DOI":"10.1001\/archopht.120.10.1268","type":"journal-article","article-title":"Reduction of intraocular pressure and glaucoma progression: results from the early manifest glaucoma trial","volume":"120","author":"Heijl","year":"2002","journal-title":"Archives of Ophthalmology"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1001\/archopht.119.8.1171","type":"journal-article","article-title":"Effects of protein kinase inhibitor, HA1077, on intraocular pressure and outflow facility in rabbit eyes","volume":"119","author":"Honjo","year":"2001","journal-title":"Archives of Ophthalmology"},{"key":"bib36","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03295-y","type":"journal-article","article-title":"Suppression of trabecular meshwork phagocytosis by norepinephrine is associated with nocturnal increase in intraocular pressure in mice","volume":"5","author":"Ikegami","year":"2022","journal-title":"Communications Biology"},{"key":"bib37","doi-asserted-by":"publisher","DOI":"10.1097\/IJG.0000000000001402","type":"journal-article","article-title":"Circadian rhythm of intraocular pressure","volume":"74","author":"Ikegami","year":"2024","journal-title":"The Journal of Physiological Sciences: JPS"},{"key":"bib38","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120774","type":"journal-article","article-title":"P38 MAP kinase inhibitor suppresses transforming growth factor-\u03b22-induced type 1 collagen production in trabecular meshwork cells","volume":"10","author":"Inoue-Mochita","year":"2015","journal-title":"PLOS ONE"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"394","DOI":"10.1007\/s13577-024-01035-4","type":"journal-article","article-title":"Role of mechanically-sensitive cation channels Piezo1 and TRPV4 in trabecular meshwork cell mechanotransduction","volume":"37","author":"Jing","year":"2024","journal-title":"Human Cell"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"3885","DOI":"10.1073\/pnas.1515895113","type":"journal-article","article-title":"Differential volume regulation and calcium signaling in two ciliary body cell types is subserved by TRPV4 channels","volume":"113","author":"Jo","year":"2016","journal-title":"PNAS"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"88","DOI":"10.1089\/jop.2013.0224","type":"journal-article","article-title":"Intraocular pressure regulation: findings of pulse-dependent trabecular meshwork motion lead to unifying concepts of intraocular pressure homeostasis","volume":"30","author":"Johnstone","year":"2014","journal-title":"Journal of Ocular Pharmacology and Therapeutics"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1093\/cvr\/cvy156","type":"journal-article","article-title":"TRPV4 increases cardiomyocyte calcium cycling and contractility yet contributes to damage in the aged heart following hypoosmotic stress","volume":"115","author":"Jones","year":"2019","journal-title":"Cardiovascular Research"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"2523","DOI":"10.1167\/iovs.12-11474","type":"journal-article","article-title":"Regulation of SPARC by transforming growth factor \u03b22 in human trabecular meshwork","volume":"54","author":"Kang","year":"2013","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib44","doi-asserted-by":"publisher","DOI":"10.3390\/bioengineering9110672","type":"journal-article","article-title":"The effect of intraocular pressure load boundary on the biomechanics of the human conventional aqueous outflow pathway","volume":"9","author":"Karimi","year":"2022","journal-title":"Bioengineering"},{"key":"bib45","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.2021.35.S1.03643","type":"journal-article","article-title":"TRPV4 regulates matrix stiffness\u2010dependent activation of YAP\/VEGFR2 signaling via Rho\/Rho kinase\/LATS1\/2 pathway","volume":"35","author":"Katari","year":"2021","journal-title":"The FASEB Journal"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"164","DOI":"10.1016\/j.exer.2018.03.001","type":"journal-article","article-title":"Consensus recommendations for trabecular meshwork cell isolation, characterization and culture","volume":"171","author":"Keller","year":"2018","journal-title":"Experimental Eye Research"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"887","DOI":"10.1212\/WNL.0b013e31820f2de3","type":"journal-article","article-title":"TRPV4 mutations and cytotoxic hypercalcemia in axonal Charcot-Marie-Tooth neuropathies","volume":"76","author":"Klein","year":"2011","journal-title":"Neurology"},{"key":"bib48","volume-title":"No Cell Is an Island: Trabecular Meshwork Ion Channels as Sensors of the Ambient Milieu\u201d in Glaucoma Research and Clinical Advances","author":"Krizaj","year":"2020","type":"book"},{"key":"bib49","doi-asserted-by":"publisher","DOI":"10.1016\/j.jlr.2021.100145","type":"journal-article","article-title":"Membrane cholesterol regulates TRPV4 function, cytoskeletal expression, and the cellular response to tension","volume":"62","author":"Lakk","year":"2021","journal-title":"Journal of Lipid Research"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"C1013","DOI":"10.1152\/ajpcell.00599.2020","type":"journal-article","article-title":"TRPV4-Rho signaling drives cytoskeletal and focal adhesion remodeling in trabecular meshwork cells","volume":"320","author":"Lakk","year":"2021","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/bs.ctm.2022.07.005","type":"journal-article","article-title":"TRPV4: Cell type-specific activation, regulation and function in the vertebrate eye","volume":"89","author":"Lapajne","year":"2022","journal-title":"Current Topics in Membranes"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"2147","DOI":"10.1167\/iovs.10-6342","type":"journal-article","article-title":"Elastic modulus determination of normal and glaucomatous human trabecular meshwork","volume":"52","author":"Last","year":"2011","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib53","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1001\/archopht.121.1.48","type":"journal-article","article-title":"Factors for glaucoma progression and the effect of treatment: the early manifest glaucoma trial","volume":"121","author":"Leske","year":"2003","journal-title":"Archives of Ophthalmology"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"1165","DOI":"10.1080\/02713683.2022.2071943","type":"journal-article","article-title":"TGF\u03b22 regulates human trabecular meshwork cell contractility via ERK and ROCK pathways with distinct signaling crosstalk dependent on the culture substrate","volume":"47","author":"Li","year":"2022","journal-title":"Current Eye Research"},{"key":"bib55","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.844342","type":"journal-article","article-title":"Extracellular matrix stiffness and TGF\u03b22 regulate YAP\/TAZ activity in human trabecular meshwork cells","volume":"10","author":"Li","year":"2022","journal-title":"Frontiers in Cell and Developmental Biology"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"13698","DOI":"10.1073\/pnas.1735416100","type":"journal-article","article-title":"Abnormal osmotic regulation in trpv4-\/- mice","volume":"100","author":"Liedtke","year":"2003","journal-title":"PNAS"},{"key":"bib57","doi-asserted-by":"publisher","first-page":"12871","DOI":"10.1073\/pnas.1323292111","type":"journal-article","article-title":"Primary cilia signaling mediates intraocular pressure sensation","volume":"111","author":"Luo","year":"2014","journal-title":"PNAS"},{"key":"bib58","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.4622","type":"journal-article","article-title":"Expression profiles of TRPV1, TRPV4, TLR4 and ERK1\/2 in the dorsal root ganglionic neurons of a cancer-induced neuropathy rat model","volume":"6","author":"Maqboul","year":"2018","journal-title":"PeerJ"},{"key":"bib59","doi-asserted-by":"publisher","DOI":"10.1186\/1744-8069-10-61","type":"journal-article","article-title":"The prophylactic effects of a traditional Japanese medicine, goshajinkigan, on paclitaxel-induced peripheral neuropathy and its mechanism of action","volume":"10","author":"Matsumura","year":"2014","journal-title":"Molecular Pain"},{"key":"bib60","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1039\/c0ib00034e","type":"journal-article","article-title":"Ultra-rapid activation of TRPV4 ion channels by mechanical forces applied to cell surface beta1 integrins","volume":"2","author":"Matthews","year":"2010","journal-title":"Integrative Biology"},{"key":"bib61","doi-asserted-by":"publisher","first-page":"6779","DOI":"10.1167\/iovs.13-12298","type":"journal-article","article-title":"Cellular fibronectin expression in human trabecular meshwork and induction by transforming growth factor-\u03b22","volume":"54","author":"Medina-Ortiz","year":"2013","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib62","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-19672","type":"journal-article","article-title":"TGF\u03b22 induces the formation of Cross-Linked Actin Networks (CLANs) in human trabecular meshwork cells through the smad and non-smad dependent pathways","volume":"58","author":"Montecchi-Palmer","year":"2017","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib63","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-52627-2","type":"journal-article","article-title":"Transcriptome analysis reveals autophagy as regulator of TGF\u03b2\/Smad-induced fibrogenesis in trabecular meshwork cells","volume":"9","author":"Nettesheim","year":"2019","journal-title":"Scientific Reports"},{"key":"bib64","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1038\/embor.2012.219","type":"journal-article","article-title":"The puzzle of TRPV4 channelopathies","volume":"14","author":"Nilius","year":"2013","journal-title":"EMBO Reports"},{"key":"bib65","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2012894118","type":"journal-article","article-title":"Thermosensitive TRPV4 channels mediate temperature-dependent microglia movement","volume":"118","author":"Nishimoto","year":"2021","journal-title":"PNAS"},{"key":"bib66","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/s0021-5155(01)00523-8","type":"journal-article","article-title":"Higher concentration of transforming growth factor-beta in aqueous humor of glaucomatous eyes and diabetic eyes","volume":"46","author":"Ochiai","year":"2002","journal-title":"Japanese Journal of Ophthalmology"},{"key":"bib67","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1073\/pnas.1319569111","type":"journal-article","article-title":"TRPV4-mediated mechanotransduction regulates the metabolic response of chondrocytes to dynamic loading","volume":"111","author":"O\u2019Conor","year":"2014","journal-title":"PNAS"},{"key":"bib68","doi-asserted-by":"publisher","first-page":"3727","DOI":"10.1167\/iovs.13-13699","type":"journal-article","article-title":"The structure of the trabecular meshwork, its connections to the ciliary muscle, and the effect of pilocarpine on outflow facility in mice","volume":"55","author":"Overby","year":"2014","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib69","doi-asserted-by":"publisher","first-page":"12856","DOI":"10.1073\/pnas.2001896117","type":"journal-article","article-title":"Molecular taxonomy of human ocular outflow tissues defined by single-cell transcriptomics","volume":"117","author":"Patel","year":"2020","journal-title":"PNAS"},{"key":"bib70","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2022461118","type":"journal-article","article-title":"Impaired TRPV4-eNOS signaling in trabecular meshwork elevates intraocular pressure in glaucoma","volume":"118","author":"Patel","year":"2021","journal-title":"PNAS"},{"key":"bib71","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23136883","type":"journal-article","article-title":"A novel mouse model of TGF\u03b22-induced ocular hypertension using lentiviral gene delivery","volume":"23","author":"Patil","year":"2022","journal-title":"International Journal of Molecular Sciences"},{"key":"bib72","doi-asserted-by":"publisher","first-page":"496","DOI":"10.1016\/j.ajpath.2014.10.023","type":"journal-article","article-title":"RhoA GTPase-induced ocular hypertension in a rodent model is associated with increased fibrogenic activity in the trabecular meshwork","volume":"185","author":"Pattabiraman","year":"2015","journal-title":"The American Journal of Pathology"},{"key":"bib73","doi-asserted-by":"publisher","first-page":"6869","DOI":"10.1113\/JP275052","type":"journal-article","article-title":"Calcium influx through TRPV4 channels modulates the adherens contacts between retinal microvascular endothelial cells","volume":"595","author":"Phuong","year":"2017","journal-title":"The Journal of Physiology"},{"key":"bib74","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1007\/s00424-012-1190-z","type":"journal-article","article-title":"Novel insights into TRPV4 function in the kidney","volume":"465","author":"Pochynyuk","year":"2013","journal-title":"Pflugers Archiv"},{"key":"bib75","doi-asserted-by":"publisher","first-page":"5225","DOI":"10.1172\/JCI75331","type":"journal-article","article-title":"TRPV4 mediates myofibroblast differentiation and pulmonary fibrosis in mice","volume":"124","author":"Rahaman","year":"2014","journal-title":"The Journal of Clinical Investigation"},{"key":"bib76","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1016\/j.exer.2016.08.023","type":"journal-article","article-title":"Role of the Rho GTPase\/Rho kinase signaling pathway in pathogenesis and treatment of glaucoma: Bench to bedside research","volume":"158","author":"Rao","year":"2017","journal-title":"Experimental Eye Research"},{"key":"bib77","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.17-22236","type":"journal-article","article-title":"Tissue transglutaminase elevates intraocular pressure in mice","volume":"58","author":"Raychaudhuri","year":"2017","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib78","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1016\/j.exer.2005.10.035","type":"journal-article","article-title":"Actin structure in the outflow tract of normal and glaucomatous eyes","volume":"84","author":"Read","year":"2007","journal-title":"Experimental Eye Research"},{"key":"bib79","doi-asserted-by":"publisher","first-page":"5665","DOI":"10.1007\/978-3-319-67199-4_101977","type":"book-chapter","article-title":"Transient receptor potential cation channel subfamily v member 4 (TRPV4)","volume-title":"Encyclopedia of Signaling Molecules","author":"Redmon","year":"2018"},{"key":"bib80","doi-asserted-by":"publisher","DOI":"10.21203\/rs.3.rs-4714050\/v1","type":"preprint","article-title":"TRPV4 subserves physiological and pathological elevations in intraocular pressure","volume-title":"Research Sqaure","author":"Redmon","year":"2024"},{"key":"bib81","doi-asserted-by":"publisher","DOI":"10.1016\/j.preteyeres.2020.100922","type":"journal-article","article-title":"The vital role for nitric oxide in intraocular pressure homeostasis","volume":"83","author":"Reina-Torres","year":"2021","journal-title":"Progress in Retinal and Eye Research"},{"key":"bib82","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1096\/fj.201900961RR","type":"journal-article","article-title":"TRPV4 receptor as a functional sensory molecule in bladder urothelium: stretch-independent, tissue-specific actions and pathological implications","volume":"34","author":"Roberts","year":"2020","journal-title":"FASEB Journal"},{"key":"bib83","doi-asserted-by":"publisher","first-page":"7089","DOI":"10.1523\/JNEUROSCI.0359-11.2011","type":"journal-article","article-title":"The polymodal ion channel transient receptor potential vanilloid 4 modulates calcium flux, spiking rate, and apoptosis of mouse retinal ganglion cells","volume":"31","author":"Ryskamp","year":"2011","journal-title":"The Journal of Neuroscience"},{"key":"bib84","doi-asserted-by":"publisher","DOI":"10.1038\/srep30583","type":"journal-article","article-title":"TRPV4 regulates calcium homeostasis, cytoskeletal remodeling, conventional outflow and intraocular pressure in the mammalian eye","volume":"6","author":"Ryskamp","year":"2016","journal-title":"Scientific Reports"},{"key":"bib85","doi-asserted-by":"publisher","first-page":"7328","DOI":"10.1167\/iovs.12-10632","type":"journal-article","article-title":"Dorsomedial\/Perifornical hypothalamic stimulation increases intraocular pressure, intracranial pressure, and the translaminar pressure gradient","volume":"53","author":"Samuels","year":"2012","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib86","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1111\/j.0022-202X.2005.23859.x","type":"journal-article","article-title":"Topical application of a peptide inhibitor of transforming growth factor-beta1 ameliorates bleomycin-induced skin fibrosis","volume":"125","author":"Santiago","year":"2005","journal-title":"The Journal of Investigative Dermatology"},{"key":"bib87","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","type":"journal-article","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nature Methods"},{"key":"bib88","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2023.109444","type":"journal-article","article-title":"Identifying new drugs and targets to treat rapidly elevated intraocular pressure for angle closure and secondary glaucomas to curb visual impairment and prevent blindness","volume":"232","author":"Sharif","year":"2023","journal-title":"Experimental Eye Research"},{"key":"bib89","doi-asserted-by":"publisher","first-page":"C562","DOI":"10.1152\/ajpcell.00187.2016","type":"journal-article","article-title":"TRPV4 ion channel is a novel regulator of dermal myofibroblast differentiation","volume":"312","author":"Sharma","year":"2017","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"bib90","doi-asserted-by":"publisher","first-page":"581","DOI":"10.1113\/jphysiol.1992.sp018941","type":"journal-article","article-title":"Potassium channels opened by noradrenaline and other transmitters in excised membrane patches of guinea-pig submucosal neurones","volume":"445","author":"Shen","year":"1992","journal-title":"The Journal of Physiology"},{"key":"bib91","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.09-4567","type":"journal-article","article-title":"Adenoviral gene transfer of active human transforming growth factor-\u03b22 elevates intraocular pressure and reduces outflow facility in rodent eyes","volume":"51","author":"Shepard","year":"2010","journal-title":"Investigative Opthalmology & Visual Science"},{"key":"bib92","doi-asserted-by":"publisher","first-page":"30115","DOI":"10.1074\/jbc.M110.141549","type":"journal-article","article-title":"Arresting a transient receptor potential (TRP) channel: beta-arrestin 1 mediates ubiquitination and functional down-regulation of TRPV4","volume":"285","author":"Shukla","year":"2010","journal-title":"The Journal of Biological Chemistry"},{"key":"bib93","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101179","type":"journal-article","article-title":"TRPV4 Channel Inhibits TGF-\u03b21-induced proliferation of hepatic stellate cells","volume":"9","author":"Song","year":"2014","journal-title":"PLOS ONE"},{"key":"bib94","doi-asserted-by":"publisher","first-page":"611","DOI":"10.3109\/02713689508998409","type":"journal-article","article-title":"Isolation and culture of human trabecular meshwork cells by extracellular matrix digestion","volume":"14","author":"Stamer","year":"1995","journal-title":"Current Eye Research"},{"key":"bib95","doi-asserted-by":"publisher","first-page":"907","DOI":"10.1007\/s00424-014-1654-4","type":"journal-article","article-title":"The role of K\u2082p channels in anaesthesia and sleep","volume":"467","author":"Steinberg","year":"2015","journal-title":"Pflugers Archiv"},{"key":"bib96","doi-asserted-by":"publisher","first-page":"2081","DOI":"10.1016\/j.ophtha.2014.05.013","type":"journal-article","article-title":"Global prevalence of glaucoma and projections of glaucoma burden through 2040: a systematic review and meta-analysis","volume":"121","author":"Tham","year":"2014","journal-title":"Ophthalmology"},{"key":"bib97","doi-asserted-by":"publisher","first-page":"3087","DOI":"10.1002\/ajmg.a.38400","type":"journal-article","article-title":"Compound heterozygous TRPV4 mutations in two siblings with a complex phenotype including severe intellectual disability and neuropathy","volume":"173","author":"Thibodeau","year":"2017","journal-title":"American Journal of Medical Genetics. Part A"},{"key":"bib98","doi-asserted-by":"publisher","DOI":"10.1186\/s12987-022-00361-9","type":"journal-article","article-title":"Lysophosphatidic acid as a CSF lipid in posthemorrhagic hydrocephalus that drives CSF accumulation via TRPV4-induced hyperactivation of NKCC1","volume":"19","author":"Toft-Bertelsen","year":"2022","journal-title":"Fluids and Barriers of the CNS"},{"key":"bib99","doi-asserted-by":"publisher","first-page":"723","DOI":"10.1006\/exer.1994.1158","type":"journal-article","article-title":"Aqueous humor in glaucomatous eyes contains an increased level of TGF-beta 2","volume":"59","author":"Tripathi","year":"1994","journal-title":"Experimental Eye Research"},{"key":"bib100","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0258911","type":"journal-article","article-title":"TRPV4 is activated by mechanical stimulation to induce prostaglandins release in trabecular meshwork, lowering intraocular pressure","volume":"16","author":"Uchida","year":"2021","journal-title":"PLOS ONE"},{"key":"bib101","doi-asserted-by":"publisher","first-page":"10339","DOI":"10.1073\/pnas.2001250117","type":"journal-article","article-title":"Cell atlas of aqueous humor outflow pathways in eyes of humans and four model species provides insight into glaucoma pathogenesis","volume":"117","author":"van Zyl","year":"2020","journal-title":"PNAS"},{"key":"bib102","doi-asserted-by":"publisher","first-page":"199","DOI":"10.1093\/intbio\/zyaa015","type":"journal-article","article-title":"Mechanical stretch sustains myofibroblast phenotype and function in microtissues through latent TGF-\u03b21 activation","volume":"12","author":"Walker","year":"2020","journal-title":"Integrative Biology"},{"key":"bib103","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/j.exer.2016.07.011","type":"journal-article","article-title":"Trabecular meshwork stiffness in glaucoma","volume":"158","author":"Wang","year":"2017","journal-title":"Experimental Eye Research"},{"key":"bib104","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1016\/j.tcb.2015.09.009","type":"journal-article","article-title":"Forcing through tumor metastasis: the interplay between tissue rigidity and epithelial-mesenchymal transition","volume":"26","author":"Wei","year":"2016","journal-title":"Trends in Cell Biology"},{"key":"bib105","doi-asserted-by":"publisher","first-page":"S53","DOI":"10.1016\/s0039-6257(02)00306-5","type":"journal-article","article-title":"Effects of prostaglandins on the aqueous humor outflow pathways","volume":"47 Suppl 1","author":"Weinreb","year":"2002","journal-title":"Survey of Ophthalmology"},{"key":"bib106","doi-asserted-by":"publisher","first-page":"2356","DOI":"10.1038\/s41440-023-01363-2","type":"journal-article","article-title":"Aortic smooth muscle TRPV4 channels regulate vasoconstriction in high salt-induced hypertension","volume":"46","author":"Wen","year":"2023","journal-title":"Hypertension Research"},{"key":"bib107","doi-asserted-by":"publisher","first-page":"911","DOI":"10.1152\/physrev.00016.2015","type":"journal-article","article-title":"TRPV4: molecular conductor of a diverse orchestra","volume":"96","author":"White","year":"2016","journal-title":"Physiological Reviews"},{"key":"bib108","doi-asserted-by":"publisher","first-page":"1447","DOI":"10.1002\/stem.3440","type":"journal-article","article-title":"Transient receptor potential vanilloid 4 as a regulator of induced pluripotent stem cell chondrogenesis","volume":"39","author":"Willard","year":"2021","journal-title":"Stem Cells"},{"key":"bib109","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1083\/jcb.200704042","type":"journal-article","article-title":"Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix","volume":"179","author":"Wipff","year":"2007","journal-title":"The Journal of Cell Biology"},{"key":"bib110","doi-asserted-by":"publisher","first-page":"39","DOI":"10.1113\/JP279776","type":"journal-article","article-title":"Emerging roles of the TRPV4 channel in bladder physiology and dysfunction","volume":"599","author":"Wu","year":"2021","journal-title":"The Journal of Physiology"},{"key":"bib111","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1093\/abbs\/gmx129","type":"journal-article","article-title":"Feedback regulation of TGF-\u03b2 signaling","volume":"50","author":"Yan","year":"2018","journal-title":"Acta Biochimica et Biophysica Sinica"},{"key":"bib112","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2022.886706","type":"journal-article","article-title":"The effects of mechanical stretch on integrins and filopodial-associated proteins in normal and glaucomatous trabecular meshwork cells","volume":"10","author":"Yang","year":"2022","journal-title":"Frontiers in Cell and Developmental Biology"},{"key":"bib113","doi-asserted-by":"publisher","first-page":"1660","DOI":"10.1085\/jgp.201812179","type":"journal-article","article-title":"TREK-1 channels regulate pressure sensitivity and calcium signaling in trabecular meshwork cells","volume":"150","author":"Yarishkin","year":"2018","journal-title":"The Journal of General Physiology"},{"key":"bib114","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1007\/978-3-319-75402-4_67","type":"journal-article","article-title":"TRPV4 does not regulate the distal retinal light response","volume":"1074","author":"Yarishkin","year":"2018","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"bib115","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1113\/JP281011","type":"journal-article","article-title":"Piezo1 channels mediate trabecular meshwork mechanotransduction and promote aqueous fluid outflow","volume":"599","author":"Yarishkin","year":"2021","journal-title":"The Journal of Physiology"},{"key":"bib116","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1016\/j.diabres.2017.08.018","type":"journal-article","article-title":"Transforming growth factor beta (TGF-\u03b2) mediates cardiac fibrosis and induces diabetic cardiomyopathy","volume":"133","author":"Yue","year":"2017","journal-title":"Diabetes Research and Clinical Practice"},{"key":"bib117","doi-asserted-by":"publisher","first-page":"C1057","DOI":"10.1152\/ajpcell.00481.2007","type":"journal-article","article-title":"Novel molecular insights into RhoA GTPase-induced resistance to aqueous humor outflow through the trabecular meshwork","volume":"295","author":"Zhang","year":"2008","journal-title":"American Journal of Physiology. Cell Physiology"},{"key":"bib118","doi-asserted-by":"publisher","first-page":"222","DOI":"10.1016\/j.neulet.2007.12.028","type":"journal-article","article-title":"A transient receptor potential vanilloid 4 contributes to mechanical allodynia following chronic compression of dorsal root ganglion in rats","volume":"432","author":"Zhang","year":"2008","journal-title":"Neuroscience Letters"},{"key":"bib119","doi-asserted-by":"publisher","first-page":"F1135","DOI":"10.1152\/ajprenal.00366.2020","type":"journal-article","article-title":"Overexpression of TGF-\u03b21 induces renal fibrosis and accelerates the decline in kidney function in polycystic kidney disease","volume":"319","author":"Zhang","year":"2020","journal-title":"American Journal of Physiology-Renal Physiology"},{"key":"bib120","doi-asserted-by":"publisher","first-page":"4023","DOI":"10.1167\/iovs.04-0535","type":"journal-article","article-title":"Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor","volume":"45","author":"Zhao","year":"2004","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"bib121","doi-asserted-by":"publisher","first-page":"1643","DOI":"10.1080\/15384101.2016.1170261","type":"journal-article","article-title":"Pro-fibrotic pathway activation in trabecular meshwork and lamina cribrosa is the main driving force of glaucoma","volume":"15","author":"Zhavoronkov","year":"2016","journal-title":"Cell Cycle"},{"key":"bib122","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21948-0","type":"journal-article","article-title":"Mechanical stress determines the configuration of TGF\u03b2 activation in articular cartilage","volume":"12","author":"Zhen","year":"2021","journal-title":"Nature Communications"},{"key":"bib123","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102042","type":"journal-article","article-title":"The role of Piezo1 in conventional aqueous humor outflow dynamics","volume":"24","author":"Zhu","year":"2021","journal-title":"iScience"},{"key":"bib124","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1161\/HYPERTENSIONAHA.122.20109","type":"journal-article","article-title":"Vascular smooth muscle TRPV4 (Transient Receptor Potential Vanilloid Family Member 4) channels regulate vasoconstriction and blood pressure in obesity","volume":"80","author":"Zhu","year":"2023","journal-title":"Hypertension"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/104894\/elife-104894-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/104894\/elife-104894-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/104894","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:36:31Z","timestamp":1787564191000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/104894"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,24]]},"references-count":124,"alternative-id":["10.7554\/eLife.104894"],"URL":"https:\/\/doi.org\/10.7554\/elife.104894","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.11.05.622187","asserted-by":"subject"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.104894.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.104894.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.104894.2","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,6,24]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP104894"}}