{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T03:15:33Z","timestamp":1788318933658,"version":"build-2803163510"},"reference-count":44,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,8,20]],"date-time":"2025-08-20T00:00:00Z","timestamp":1755648000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFD1400100"],"award-info":[{"award-number":["2022YFD1400100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32072448"],"award-info":[{"award-number":["32072448"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Modern fungicides have made significant contributions to crop disease management, but the development of resistant fungal strains has caused their failure in disease control. Therefore, developing fungicides with novel action mechanisms is the most effective measure to manage resistance. Quinofumelin, a novel quinoline fungicide, exhibits exceptional antifungal activity against phytopathogens. However, there is currently no available information on its mechanism of action. Here, we used transcriptome and metabolome analysis to observe a co-enrichment pattern of differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) within pyrimidine biosynthesis pathway (PBP), identifying down-regulation of dihydroorotate dehydrogenase (DHODH). Exogenous uridine monophosphate (UMP), uridine, or uracil (metabolites in PBP) successfully restored quinofumelin-induced inhibition of mycelial growth in\n                    <jats:italic>Fusarium graminearum<\/jats:italic>\n                    and\n                    <jats:italic>Fusarium asiaticum<\/jats:italic>\n                    . Additionally, the deletion of\n                    <jats:italic>FgDHODHII<\/jats:italic>\n                    was determined to be lethal; however, mycelial growth of \u0394FgDHODHII mutants could be restored by adding UMP, uridine, or uracil. These findings indicate that the deficiencies in\n                    <jats:italic>FgDHODHII<\/jats:italic>\n                    are functionally equivalent to complete inhibition of its activity by quinofumelin. Finally, molecular docking, surface plasmon resonance (SPR), and microscale thermophoresis (MST) results strongly support the precise interaction between quinofumelin and FgDHODHII. Collectively, these findings provide compelling evidence for the involvement of de novo uracil biosynthesis as a mechanism of action for quinofumelin while identifying FgDHODHII as its specific target.\n                  <\/jats:p>","DOI":"10.7554\/elife.105892","type":"journal-article","created":{"date-parts":[[2025,3,7]],"date-time":"2025-03-07T11:25:07Z","timestamp":1741346707000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.105892","source":"Crossref","is-referenced-by-count":1,"title":["Action mechanism of a novel agrichemical quinofumelin against Fusarium graminearum"],"prefix":"10.7554","volume":"14","author":[{"given":"Qian","family":"Xiu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-2877-6950","authenticated-orcid":true,"given":"Xiaoru","family":"Yin","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"given":"Yuanyuan","family":"Chen","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Science, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"software","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4779-3041","authenticated-orcid":true,"given":"Ziyang","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Yushuai","family":"Mao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"data-curation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"}]},{"given":"Tianshi","family":"Wang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"College of Science, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"software","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0190-7574","authenticated-orcid":true,"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Mingguo","family":"Zhou","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4183-8729","authenticated-orcid":true,"given":"Yabing","family":"Duan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05td3s095","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection, Nanjing Agricultural University","place":["Nanjing, China"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2025,8,20]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"17790","DOI":"10.1039\/c5cp02016f","type":"journal-article","article-title":"Insights into the mechanism of oxidation of dihydroorotate to orotate catalysed by human class 2 dihydroorotate dehydrogenase: a QM\/MM free energy study","volume":"17","author":"Alves","year":"2015","journal-title":"Physical Chemistry Chemical Physics"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"W202","DOI":"10.1093\/nar\/gkp335","type":"journal-article","article-title":"MEME SUITE: tools for motif discovery and searching","volume":"37","author":"Bailey","year":"2009","journal-title":"Nucleic Acids Research"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"D506","DOI":"10.1093\/nar\/gky1049","type":"journal-article","article-title":"UniProt: a worldwide hub of protein knowledge","volume":"47","author":"Bateman","year":"2019","journal-title":"Nucleic Acids Research"},{"key":"bib4","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2020.165759","type":"journal-article","article-title":"Dihydroorotate dehydrogenase in oxidative phosphorylation and cancer","volume":"1866","author":"Boukalova","year":"2020","journal-title":"Biochimica et Biophysica Acta. Molecular Basis of Disease"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"82","DOI":"10.1016\/0003-9861(76)90143-0","type":"journal-article","article-title":"The cellular location of dihydroorotate dehydrogenase: relation to de novo biosynthesis of pyrimidines","volume":"176","author":"Chen","year":"1976","journal-title":"Archives of Biochemistry and Biophysics"},{"key":"bib6","first-page":"3521","type":"journal-article","article-title":"Inhibition of dihydroorotate dehydrogenase activity by brequinar sodium","volume":"52","author":"Chen","year":"1992","journal-title":"Cancer Research"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"1194","DOI":"10.1016\/j.molp.2020.06.009","type":"journal-article","article-title":"TBtools: An integrative toolkit developed for interactive analyses of big biological data","volume":"13","author":"Chen","year":"2020","journal-title":"Molecular Plant"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/0006-3002(63)91816-x","type":"journal-article","article-title":"The kinetics of enzyme-catalyzed reactions with two or more substrates or products. III. Prediction of initial velocity and inhibition patterns by inspection","volume":"67","author":"Cleland","year":"1963","journal-title":"Biochimica et Biophysica Acta"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"3086","DOI":"10.7150\/jca.14835","type":"journal-article","article-title":"Dihydroorotate dehydrogenase inhibitors target c-Myc and arrest melanoma, myeloma and lymphoma cells at S-phase","volume":"8","author":"Dorasamy","year":"2017","journal-title":"Journal of Cancer"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1016\/j.pestbp.2018.11.006","type":"journal-article","article-title":"Benzimidazole- and QoI-resistance in Corynespora cassiicola populations from greenhouse-cultivated cucumber: An emerging problem in China","volume":"153","author":"Duan","year":"2019","journal-title":"Pesticide Biochemistry and Physiology"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"467","DOI":"10.7754\/Clin.Lab.2017.170922","type":"journal-article","article-title":"High-level prokaryotic expression and purification of death domain superfamily with MBP tag","volume":"64","author":"Fang","year":"2018","journal-title":"Clinical Laboratory"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1002\/jbio.201200015","type":"journal-article","article-title":"Surface plasmon resonance based biosensor technique: a review","volume":"5","author":"Guo","year":"2012","journal-title":"Journal of Biophotonics"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1584\/jpestics.D22-027","type":"journal-article","article-title":"The target site of the novel fungicide quinofumelin, Pyricularia oryzae class II dihydroorotate dehydrogenase","volume":"47","author":"Higashimura","year":"2022","journal-title":"Journal of Pesticide Science"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1584\/jpestics.D22-042","type":"journal-article","article-title":"Design and biological activity of a novel fungicide, quinofumelin","volume":"48","author":"Ito","year":"2023","journal-title":"Journal of Pesticide Science"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","type":"journal-article","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"2086","DOI":"10.1111\/ppa.13441","type":"journal-article","article-title":"Fungicide resistance status and chemical control options for the brassica pathogen Pyrenopeziza brassicae","volume":"70","author":"King","year":"2021","journal-title":"Plant Pathology"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"428","DOI":"10.1016\/j.ejps.2012.11.016","type":"journal-article","article-title":"Analysis of the landscape of biologically-derived pharmaceuticals in Europe: dominant production systems, molecule types on the rise and approval trends","volume":"48","author":"Kyriakopoulos","year":"2013","journal-title":"European Journal of Pharmaceutical Sciences"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1016\/B978-0-12-821035-2.00047-4","type":"book-chapter","article-title":"Chapter 4 - agrochemical disease control: the story so far","volume-title":"Recent Highlights in the Discovery and Optimization of Crop Protection Products","author":"Lamberth","year":"2021"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1021\/acsmedchemlett.2c00113","type":"journal-article","article-title":"Latest research trends in agrochemical fungicides: any learnings for pharmaceutical antifungals?","volume":"13","author":"Lamberth","year":"2022","journal-title":"ACS Medicinal Chemistry Letters"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1016\/bs.aambs.2014.09.001","type":"journal-article","article-title":"The evolution of fungicide resistance","volume":"90","author":"Lucas","year":"2015","journal-title":"Advances in Applied Microbiology"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"586","DOI":"10.1038\/s41586-021-03539-7","type":"journal-article","article-title":"DHODH-mediated ferroptosis defence is a targetable vulnerability in cancer","volume":"593","author":"Mao","year":"2021","journal-title":"Nature"},{"key":"bib22","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2023.117797","type":"journal-article","article-title":"Genetic variation of drug target CYP51 conferring resistance to ergosterol biosynthesis inhibitors in Botrytis cinerea, causing lily gray mould","volume":"208","author":"Mao","year":"2024","journal-title":"Industrial Crops and Products"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"D205","DOI":"10.1093\/nar\/gkn845","type":"journal-article","article-title":"CDD: specific functional annotation with the Conserved Domain Database","volume":"37","author":"Marchler-Bauer","year":"2009","journal-title":"Nucleic Acids Research"},{"key":"bib24","doi-asserted-by":"publisher","DOI":"10.1094\/APSnetFeature-2008-0308","type":"journal-article","article-title":"A short history of fungicides","volume":"1","author":"Morton","year":"2008","journal-title":"APSnet Feature Articles"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"1211","DOI":"10.1016\/s0969-2126(02)00831-6","type":"journal-article","article-title":"E. coli dihydroorotate dehydrogenase reveals structural and functional distinctions between different classes of dihydroorotate dehydrogenases","volume":"10","author":"N\u00f8rager","year":"2002","journal-title":"Structure"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1080\/10408347.2014.881250","type":"journal-article","article-title":"Surface plasmon resonance (SPR) biosensors in pharmaceutical analysis","volume":"45","author":"Olaru","year":"2015","journal-title":"Critical Reviews in Analytical Chemistry"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"1650","DOI":"10.1038\/nprot.2016.095","type":"journal-article","article-title":"Transcript-level expression analysis of RNA-seq experiments with HISAT, StringTie and Ballgown","volume":"11","author":"Pertea","year":"2016","journal-title":"Nature Protocols"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1007\/978-1-60327-567-5_5","type":"book-chapter","article-title":"Surface plasmon resonance biosensing","volume-title":"Biosensors and Biodetection","author":"Piliarik","year":"2009"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1128\/JB.00422-15","type":"journal-article","article-title":"An extended cyclic Di-GMP network in the predatory bacterium bdellovibrio bacteriovorus","volume":"198","author":"Rotem","year":"2016","journal-title":"Journal of Bacteriology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1094\/PHYTO-07-20-0313-IA","type":"journal-article","article-title":"Advances in understanding fungicide resistance in Botrytis cinerea in China","volume":"111","author":"Shao","year":"2021","journal-title":"Phytopathology"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1111\/mpp.12887","type":"journal-article","article-title":"Capping proteins regulate fungal development, DON-toxisome formation and virulence in Fusarium graminearum","volume":"21","author":"Tang","year":"2020","journal-title":"Molecular Plant Pathology"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"2567","DOI":"10.1094\/PDIS-08-20-1821-RE","type":"journal-article","article-title":"Antifungal activity and biological characteristics of the novel fungicide quinofumelin against Sclerotinia sclerotiorum","volume":"105","author":"Tao","year":"2021","journal-title":"Plant Disease"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1584\/jpestics.D20-201","type":"journal-article","article-title":"Development of novel pesticides in the 21st century","volume":"45","author":"Umetsu","year":"2020","journal-title":"Journal of Pesticide Science"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1038\/s41564-023-01542-4","type":"journal-article","article-title":"Aspergillus fumigatus strains that evolve resistance to the agrochemical fungicide ipflufenoquin in vitro are also resistant to olorofim","volume":"9","author":"van Rhijn","year":"2024","journal-title":"Nature Microbiology"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"367","DOI":"10.1016\/j.tcb.2015.12.007","type":"journal-article","article-title":"Understanding Ubiquinone","volume":"26","author":"Wang","year":"2016","journal-title":"Trends in Cell Biology"},{"key":"bib36","doi-asserted-by":"publisher","DOI":"10.3390\/biotech12040064","type":"journal-article","article-title":"Fungicide resistance in Botrytis spp. and regional strategies for its management in Northern European strawberry production","volume":"12","author":"Weber","year":"2023","journal-title":"BioTech"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1046\/j.1364-3703.2003.00165.x","type":"journal-article","article-title":"Quinoxyfen perturbs signal transduction in barley powdery mildew (Blumeria graminis f.sp. hordei)","volume":"4","author":"Wheeler","year":"2003","journal-title":"Molecular Plant Pathology"},{"key":"bib38","doi-asserted-by":"publisher","DOI":"10.3390\/toxins13050348","type":"journal-article","article-title":"Antifungal activity of quinofumelin against Fusarium graminearum and its inhibitory effect on DON biosynthesis","volume":"13","author":"Xiu","year":"2021","journal-title":"Toxins"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"10520","DOI":"10.1021\/acs.chemrev.8b00728","type":"journal-article","article-title":"Concepts of Artificial intelligence for computer-assisted drug discovery","volume":"119","author":"Yang","year":"2019","journal-title":"Chemical Reviews"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1042\/EBC20200172","type":"journal-article","article-title":"Escherichia coli as a platform microbial host for systems metabolic engineering","volume":"65","author":"Yang","year":"2021","journal-title":"Essays in Biochemistry"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1111\/mpp.12258","type":"journal-article","article-title":"Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum","volume":"17","author":"Zhang","year":"2016","journal-title":"Molecular Plant Pathology"},{"key":"bib42","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00572-w","type":"journal-article","article-title":"Small molecules in targeted cancer therapy: advances, challenges, and future perspectives","volume":"6","author":"Zhong","year":"2021","journal-title":"Signal Transduction and Targeted Therapy"},{"key":"bib43","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1134\/S0003683814040140","type":"journal-article","article-title":"Molecular cloning, prokaryotic expression and promoter analysis of squalene synthase gene from Schizochytrium Limacinum","volume":"50","author":"Zhu","year":"2014","journal-title":"Applied Biochemistry and Microbiology"},{"key":"dataset1","doi-asserted-by":"publisher","DOI":"10.5061\/dryad.n5tb2rc7r","type":"dataset","article-title":"Action mechanism of a novel agrichemical quinofumelin against Fusarium graminearum","volume-title":"Dryad Digital Repository","author":"Xiu","year":"2025","unstructured":"Xiu Q, Yin XR, Chen YY, Zhang ZY, Mao YS, Wang TS, Zhang J, Zhou MG, Duan YB. 2025. Action mechanism of a novel agrichemical quinofumelin against Fusarium graminearum. Dryad Digital Repository."}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/105892\/elife-105892-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/105892\/elife-105892-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/105892","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:30:59Z","timestamp":1787563859000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/105892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,20]]},"references-count":44,"alternative-id":["10.7554\/eLife.105892"],"URL":"https:\/\/doi.org\/10.7554\/elife.105892","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.01.13.632717","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2025.01.13.632717","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.105892.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.105892.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.105892.2","asserted-by":"subject"}],"is-supplemented-by":[{"id-type":"doi","id":"10.5061\/dryad.n5tb2rc7r","asserted-by":"subject"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,20]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP105892"}}