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In mammals and humans, these functions are generally thought to be produced by different populations of neurons. Here, we investigated in the larva of\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    whether both these functions can be induced by the same individual dopaminergic neurons in the central brain. Focusing on the dopaminergic neurons of the DL1 cluster, we asked whether the optogenetic activation of individual neurons established associative punishment and\/or safety memories, controlled the retrieval of the established memories, and acutely modulated locomotion. We found that each neuron had a unique yet overlapping set of behavioural effects. Several individual neurons both established a memory and modulated acute locomotion by increasing the animals\u2019 bending and decreasing their velocity. Our results demonstrate that individual dopaminergic neurons can fulfil a surprisingly broad range of functions in different behavioural contexts. Given the highly conserved roles of the dopaminergic system across the animal kingdom, this study raises the question whether a similarly diverse functionality can be found also in other animals, including humans.\n                  <\/jats:p>","DOI":"10.7554\/elife.106148","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T10:30:27Z","timestamp":1744367427000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.106148","source":"Crossref","is-referenced-by-count":0,"title":["Individual dopaminergic neurons induce unique, yet overlapping combinations of behavioural modulations, including safety learning, memory retrieval, and acute locomotion"],"prefix":"10.7554","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9245-7494","authenticated-orcid":true,"given":"Naoko","family":"Toshima","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01zwmgk08","id-type":"ROR","asserted-by":"publisher"}],"name":"Leibniz Institute for 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