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To address this issue, we have developed long-term, continuous, fast and high-resolution imaging of growth cone dynamics from axon growth to synapse formation in cultured Drosophila brains. Using R7 photoreceptor neurons as a model we show that &gt;90% of the growth cone filopodia exhibit fast, stochastic dynamics that persist despite ongoing stepwise layer formation. Correspondingly, R7 growth cones stabilize early and change their final position by passive dislocation. N-Cadherin controls both fast filopodial dynamics and growth cone stabilization. Surprisingly, loss of N-Cadherin causes no primary targeting defects, but destabilizes R7 growth cones to jump between correct and incorrect layers. Hence, growth cone dynamics can influence wiring specificity without a direct role in target recognition and implement simple rules during circuit assembly.<\/jats:p>","DOI":"10.7554\/elife.10721","type":"journal-article","created":{"date-parts":[[2015,10,29]],"date-time":"2015-10-29T07:35:45Z","timestamp":1446104145000},"source":"Crossref","is-referenced-by-count":86,"title":["Filopodial dynamics and growth cone stabilization in Drosophila visual circuit development"],"prefix":"10.7554","volume":"4","author":[{"given":"Mehmet Neset","family":"\u00d6zel","sequence":"first","affiliation":[{"name":"Department of Physiology, University of Texas Southwestern Medical Center, Dallas, United States"},{"name":"Division of Neurobiology, Institute for Biology, Freie Universit\u00e4t Berlin, Berlin, Germany"},{"name":"NeuroCure Cluster of Excellence, Charite Universit\u00e4tsmedizin Berlin, Berlin, 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