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Resistance has arisen to the trypanocidal drugs pentamidine and melarsoprol through mutations in the aquaglyceroporin TbAQP2 that prevent their uptake. Here, we use cryogenic electron microscopy to determine the structure of TbAQP2 from\n                    <jats:italic>T. brucei<\/jats:italic>\n                    , bound to either the substrate glycerol or to the sleeping sickness drugs, pentamidine or melarsoprol. The drugs bind within the AQP2 channel at a site completely overlapping that of glycerol. Mutations leading to a drug-resistant phenotype were found in the channel lining. Molecular dynamics (MD) simulations showed the channel can be traversed by pentamidine, with a low energy binding site at the centre of the channel, flanked by regions of high energy association at the extracellular and intracellular ends. Drug-resistant TbAQP2 mutants are still predicted to bind pentamidine, but the much weaker binding in the centre of the channel observed in the MD simulations would be insufficient to compensate for the high energy processes of ingress and egress, hence impairing transport at pharmacologically relevant concentrations. The structures of drug-bound TbAQP2 represent a novel paradigm for drug\u2013transporter interactions and are a new mechanism for targeting drugs in pathogens and human cells.\n                  <\/jats:p>","DOI":"10.7554\/elife.107460","type":"journal-article","created":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T12:30:35Z","timestamp":1751459435000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.107460","source":"Crossref","is-referenced-by-count":1,"title":["Insights from aquaporin structures into drug-resistant sleeping sickness"],"prefix":"10.7554","volume":"14","author":[{"given":"Modestas","family":"Matusevicius","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/013meh722","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Medicine, University of Cambridge, Victor Phillip Dahdaleh Building, Heart and Lung Research Institute","place":["Cambridge, United 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