{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T10:31:38Z","timestamp":1787567498555,"version":"build-2736575974"},"reference-count":56,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T00:00:00Z","timestamp":1762905600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21K19569"],"award-info":[{"award-number":["JP21K19569"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP21K19569"],"award-info":[{"award-number":["JP21K19569"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP22H03214"],"award-info":[{"award-number":["JP22H03214"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP22H03214"],"award-info":[{"award-number":["JP22H03214"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP21H05033"],"award-info":[{"award-number":["JP21H05033"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP21H05033"],"award-info":[{"award-number":["JP21H05033"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP22H04922"],"award-info":[{"award-number":["JP22H04922"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP22H04922"],"award-info":[{"award-number":["JP22H04922"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["JP23K20043"],"award-info":[{"award-number":["JP23K20043"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP23K20043"],"award-info":[{"award-number":["JP23K20043"]}],"id":[{"id":"https:\/\/ror.org\/00hhkn466","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"crossref","award":["10.52926\/jpmjfr211f"],"award-info":[{"award-number":["10.52926\/jpmjfr211f"],"id":[{"id":"10.52926\/jpmjfr211f","id-type":"DOI","asserted-by":"publisher"}]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"crossref"}]},{"award":["10.52926\/jpmjfr211f"],"award-info":[{"award-number":["10.52926\/jpmjfr211f"],"id":[{"id":"10.52926\/jpmjfr211f","id-type":"DOI","asserted-by":"publisher"}]}],"id":[{"id":"https:\/\/ror.org\/00097mb19","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002241","name":"Japan Science and Technology Agency","doi-asserted-by":"crossref","award":["10.52926\/jpmjcr21n1"],"award-info":[{"award-number":["10.52926\/jpmjcr21n1"],"id":[{"id":"10.52926\/jpmjcr21n1","id-type":"DOI","asserted-by":"publisher"}]}],"id":[{"id":"10.13039\/501100002241","id-type":"DOI","asserted-by":"crossref"}]},{"award":["10.52926\/jpmjcr21n1"],"award-info":[{"award-number":["10.52926\/jpmjcr21n1"],"id":[{"id":"10.52926\/jpmjcr21n1","id-type":"DOI","asserted-by":"publisher"}]}],"id":[{"id":"https:\/\/ror.org\/00097mb19","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP23jf0126001"],"award-info":[{"award-number":["JP23jf0126001"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP23jf0126001"],"award-info":[{"award-number":["JP23jf0126001"]}],"id":[{"id":"https:\/\/ror.org\/004rtk039","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP23fa627002"],"award-info":[{"award-number":["JP23fa627002"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP23fa627002"],"award-info":[{"award-number":["JP23fa627002"]}],"id":[{"id":"https:\/\/ror.org\/004rtk039","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"crossref","award":["JP23fa627006"],"award-info":[{"award-number":["JP23fa627006"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"crossref"}]},{"award":["JP23fa627006"],"award-info":[{"award-number":["JP23fa627006"]}],"id":[{"id":"https:\/\/ror.org\/004rtk039","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"crossref"}]},{"id":[{"id":"https:\/\/ror.org\/02y123g31","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Eunice Kennedy Shriver National Institute of Child Health and Human Development","award":["R01HD088412"],"award-info":[{"award-number":["R01HD088412"]}]},{"award":["R01HD088412"],"award-info":[{"award-number":["R01HD088412"]}],"id":[{"id":"https:\/\/ror.org\/04byxyr05","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill & Melinda Gates Foundation","doi-asserted-by":"crossref","award":["INV-001902"],"award-info":[{"award-number":["INV-001902"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"crossref"}]},{"award":["INV-001902"],"award-info":[{"award-number":["INV-001902"]}],"id":[{"id":"https:\/\/ror.org\/0456r8d26","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Osaka University","award":["OU Master Plan Implementation Project"],"award-info":[{"award-number":["OU Master Plan Implementation Project"]}]},{"award":["OU Master Plan Implementation Project"],"award-info":[{"award-number":["OU Master Plan Implementation Project"]}],"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":["elifesciences.org"],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>\n                    Despite the recognized importance of glycans in biological phenomena, their complex roles in spermatogenesis and sperm function remain unclear. SLC35G3, a 10-transmembrane protein specifically found in early round spermatids, belongs to the sugar-nucleotide transporter family, indicating its involvement in glycan formation. In this study, we found that\n                    <jats:italic>Slc35g3<\/jats:italic>\n                    knockout male mice were sterile due to impaired sperm functions in uterotubal junction passage, zona pellucida binding, and oocyte fusion. Mouse SLC35G3 has UDP-GlcNAc transporter activity, and its ablation caused abnormal processing of the sperm plasma membrane and acrosome membrane proteins. Reported human\n                    <jats:italic>SLC35G3<\/jats:italic>\n                    mutations (F267L and T179HfsTer27) diminished the UDP-GlcNAc transporter activity of SLC35G3, implying infertility risks in males carrying these mutations. Our findings unveil the vital roles of SLC35G3 in the glycan formation of sperm membrane proteins critical for sperm-fertilizing ability.\n                  <\/jats:p>","DOI":"10.7554\/elife.107494","type":"journal-article","created":{"date-parts":[[2025,7,21]],"date-time":"2025-07-21T16:31:58Z","timestamp":1753115518000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.107494","source":"Crossref","is-referenced-by-count":0,"title":["SLC35G3 is a UDP-N-acetylglucosamine transporter for sperm glycoprotein formation and underpins male fertility in mice"],"prefix":"10.7554","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3927-3076","authenticated-orcid":true,"given":"Daisuke","family":"Mashiko","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, The University of Osaka","place":["Osaka, Japan"]},{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Immunology Frontier Research Center, The University of Osaka","place":["Osaka, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Shingo","family":"Tonai","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, The University of Osaka","place":["Osaka, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"investigation","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4758-5803","authenticated-orcid":true,"given":"Haruhiko","family":"Miyata","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, The University of Osaka","place":["Osaka, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1445-8632","authenticated-orcid":true,"given":"Martin M","family":"Matzuk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02pttbw34","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Immunology, Baylor College of Medicine","place":["Houston, United States"]},{"id":[{"id":"https:\/\/ror.org\/02pttbw34","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Drug Discovery, Baylor College of Medicine","place":["Houston, United States"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"visualization","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9859-6217","authenticated-orcid":true,"given":"Masahito","family":"Ikawa","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Experimental Genome Research, Research Institute for Microbial Diseases, The University of Osaka","place":["Osaka, Japan"]},{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Immunology Frontier Research Center, The University of Osaka","place":["Osaka, Japan"]},{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Advanced Modalities and Drug Delivery System, The University of Osaka","place":["Osaka, Japan"]},{"id":[{"id":"https:\/\/ror.org\/035t8zc32","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Disease Education and Research, The University of Osaka","place":["Osaka, Japan"]},{"id":[{"id":"https:\/\/ror.org\/057zh3y96","id-type":"ROR","asserted-by":"publisher"}],"name":"The Institute of Medical Science, The University of Tokyo","place":["Tokyo, Japan"]}],"role":[{"role":"author","vocabulary":"crossref"},{"role":"corresponding-author","vocabulary":"crossref"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"methodology","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]}],"member":"4374","published-online":{"date-parts":[[2025,11,12]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"2430","DOI":"10.1016\/j.bbamcr.2013.04.001","type":"journal-article","article-title":"N-linked protein glycosylation in the ER","volume":"1833","author":"Aebi","year":"2013","journal-title":"Biochimica et Biophysica Acta"},{"key":"bib2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.45221","type":"journal-article","article-title":"Structural basis for mammalian nucleotide sugar transport","volume":"8","author":"Ahuja","year":"2019","journal-title":"eLife"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/978-1-4684-5913-5_3","type":"journal-article","article-title":"Effects of elevated temperature on the epididymis and testis: experimental studies","volume":"286","author":"Bedford","year":"1991","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"2993","DOI":"10.1021\/acschembio.1c00689","type":"journal-article","article-title":"A useful guide to lectin binding: machine-learning directed annotation of 57 unique lectin specificities","volume":"17","author":"Bojar","year":"2022","journal-title":"ACS Chemical Biology"},{"key":"bib5","doi-asserted-by":"publisher","DOI":"10.1126\/science.adg7492","type":"journal-article","article-title":"Accurate proteome-wide missense variant effect prediction with AlphaMissense","volume":"381","author":"Cheng","year":"2023","journal-title":"Science"},{"key":"bib6","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abo6049","type":"journal-article","article-title":"C11orf94\/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly","volume":"8","author":"Contreras","year":"2022","journal-title":"Science Advances"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"3583","DOI":"10.1242\/dev.081778","type":"journal-article","article-title":"SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia","volume":"139","author":"Fujihara","year":"2012","journal-title":"Development"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"8111","DOI":"10.1073\/pnas.1222166110","type":"journal-article","article-title":"Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa","volume":"110","author":"Fujihara","year":"2013","journal-title":"PNAS"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1095\/biolreprod.113.112888","type":"journal-article","article-title":"GPI-anchored protein complex, LY6K\/TEX101, is required for sperm migration into the oviduct and male fertility in mice","volume":"90","author":"Fujihara","year":"2014","journal-title":"Biology of Reproduction"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1538\/expanim.17-0153","type":"journal-article","article-title":"Factors controlling sperm migration through the oviduct revealed by gene-modified mouse models","volume":"67","author":"Fujihara","year":"2018","journal-title":"Experimental Animals"},{"key":"bib11","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.221481","type":"journal-article","article-title":"Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice","volume":"131","author":"Fujihara","year":"2018","journal-title":"Journal of Cell Science"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"18498","DOI":"10.1073\/pnas.1908736116","type":"journal-article","article-title":"Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice","volume":"116","author":"Fujihara","year":"2019","journal-title":"PNAS"},{"key":"bib13","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2108777118","type":"journal-article","article-title":"The conserved fertility factor SPACA4\/Bouncer has divergent modes of action in vertebrate fertilization","volume":"118","author":"Fujihara","year":"2021","journal-title":"PNAS"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"2552","DOI":"10.1073\/pnas.95.5.2552","type":"journal-article","article-title":"Angiotensin-converting enzyme and male fertility","volume":"95","author":"Hagaman","year":"1998","journal-title":"PNAS"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1538\/expanim.59.105","type":"journal-article","article-title":"Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo","volume":"59","author":"Hasuwa","year":"2010","journal-title":"Experimental Animals"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1038\/42484","type":"journal-article","article-title":"The putative chaperone calmegin is required for sperm fertility","volume":"387","author":"Ikawa","year":"1997","journal-title":"Nature"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1038\/nature03362","type":"journal-article","article-title":"The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs","volume":"434","author":"Inoue","year":"2005","journal-title":"Nature"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"910","DOI":"10.1016\/j.bbrc.2008.10.073","type":"journal-article","article-title":"Putative sperm fusion protein IZUMO and the role of N-glycosylation","volume":"377","author":"Inoue","year":"2008","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"583","DOI":"10.1038\/s41586-021-03819-2","type":"journal-article","article-title":"Highly accurate protein structure prediction with AlphaFold","volume":"596","author":"Jumper","year":"2021","journal-title":"Nature"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"5634","DOI":"10.1074\/jbc.M510558200","type":"journal-article","article-title":"Mouse sperm lacking ADAM1b\/ADAM2 fertilin can fuse with the egg plasma membrane and effect fertilization","volume":"281","author":"Kim","year":"2006","journal-title":"The Journal of Biological Chemistry"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1038\/nmeth1204-249","type":"journal-article","article-title":"Preparation, isolation and characterization of stage-specific spermatogenic cells for cellular and molecular analysis","volume":"1","author":"Kotaja","year":"2004","journal-title":"Nature Methods"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1038\/375146a0","type":"journal-article","article-title":"Male-female differences in fertility and blood pressure in ACE-deficient mice","volume":"375","author":"Krege","year":"1995","journal-title":"Nature"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1016\/0146-664X(82)90034-X","type":"journal-article","article-title":"Elliptic Fourier features of a closed contour","volume":"18","author":"Kuhl","year":"1982","journal-title":"Computer Graphics and Image Processing"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"6794","DOI":"10.1128\/MCB.01029-07","type":"journal-article","article-title":"Loss of zona pellucida binding proteins in the acrosomal matrix disrupts acrosome biogenesis and sperm morphogenesis","volume":"27","author":"Lin","year":"2007","journal-title":"Molecular and Cellular Biology"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1093\/biolre\/ioz103","type":"journal-article","article-title":"CRISPR\/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice\u2020","volume":"101","author":"Lu","year":"2019","journal-title":"Biology of Reproduction"},{"key":"bib26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2207263120","type":"journal-article","article-title":"1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm\u2013egg interaction","volume":"120","author":"Lu","year":"2023","journal-title":"PNAS"},{"key":"bib27","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.75373","type":"journal-article","article-title":"Oligogenic heterozygous inheritance of sperm abnormalities in mouse","volume":"11","author":"Martinez","year":"2022","journal-title":"eLife"},{"key":"bib28","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.3913","type":"journal-article","article-title":"Mouse spermatozoa with higher fertilization rates have thinner nuclei","volume":"5","author":"Mashiko","year":"2017","journal-title":"PeerJ"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1083\/jcb.106.4.1049","type":"journal-article","article-title":"The role of the asparagine-linked oligosaccharides of the alpha subunit in the secretion and assembly of human chorionic gonadotrophin","volume":"106","author":"Matzuk","year":"1988","journal-title":"The Journal of Cell Biology"},{"key":"bib30","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1016\/S0021-9258(19)81628-9","type":"journal-article","article-title":"Site specificity of the chorionic gonadotropin N-linked oligosaccharides in signal transduction","volume":"264","author":"Matzuk","year":"1989","journal-title":"The Journal of Biological Chemistry"},{"key":"bib31","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1126\/science.aad0836","type":"journal-article","article-title":"Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive","volume":"350","author":"Miyata","year":"2015","journal-title":"Science"},{"key":"bib32","doi-asserted-by":"publisher","DOI":"10.1242\/dev.199644","type":"journal-article","article-title":"FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice","volume":"148","author":"Morohoshi","year":"2021","journal-title":"Development"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1016\/0378-1119(91)90434-d","type":"journal-article","article-title":"Efficient selection for high-expression transfectants with a novel eukaryotic vector","volume":"108","author":"Niwa","year":"1991","journal-title":"Gene"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"11493","DOI":"10.1073\/pnas.1922650117","type":"journal-article","article-title":"Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice","volume":"117","author":"Noda","year":"2020","journal-title":"PNAS"},{"key":"bib35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ggedit.2022.100014","type":"journal-article","article-title":"Genome editing in mice and its application to the study of spermatogenesis","volume":"3\u20134","author":"Oura","year":"2022","journal-title":"Gene and Genome Editing"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"2955","DOI":"10.1242\/dev.077982","type":"journal-article","article-title":"Absence of Dpy19l2, a new inner nuclear membrane protein, causes globozoospermia in mice by preventing the anchoring of the acrosome to the nucleus","volume":"139","author":"Pierre","year":"2012","journal-title":"Development"},{"key":"bib37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000202","type":"journal-article","article-title":"PlotsOfData\u2014A web app for visualizing data together with their summaries","volume":"17","author":"Postma","year":"2019","journal-title":"PLOS Biology"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1093\/glycob\/cwv003","type":"journal-article","article-title":"UDP-N-acetyl-\u03b1-D-galactosamine: polypeptide N-acetylgalactosaminyltransferases: completion of the family tree","volume":"22","author":"Raman","year":"2012","journal-title":"Glycobiology"},{"key":"bib39","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-020-00826-z","type":"journal-article","article-title":"Large-scale discovery of male reproductive tract-specific genes through analysis of RNA-seq datasets","volume":"18","author":"Robertson","year":"2020","journal-title":"BMC Biology"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"D735","DOI":"10.1093\/nar\/gkm1005","type":"journal-article","article-title":"TreeFam: 2008 Update","volume":"36","author":"Ruan","year":"2008","journal-title":"Nucleic Acids Research"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1038\/s41580-020-00294-x","type":"journal-article","article-title":"Global view of human protein glycosylation pathways and functions","volume":"21","author":"Schjoldager","year":"2020","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"bib42","doi-asserted-by":"publisher","first-page":"1445","DOI":"10.1095\/biolreprod61.6.1445","type":"journal-article","article-title":"Male mice deficient for germ-cell cyritestin are infertile","volume":"61","author":"Shamsadin","year":"1999","journal-title":"Biology of Reproduction"},{"key":"bib43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2018355118","type":"journal-article","article-title":"ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility","volume":"118","author":"Shimada","year":"2021","journal-title":"PNAS"},{"key":"bib44","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1073\/pnas.1310777111","type":"journal-article","article-title":"A heterozygous mutation of GALNTL5 affects male infertility with impairment of sperm motility","volume":"111","author":"Takasaki","year":"2014","journal-title":"PNAS"},{"key":"bib45","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.semcdb.2014.12.001","type":"book-chapter","article-title":"N-linked sugar-regulated protein folding and quality control in the ER","volume-title":"Seminars in Cell & Developmental Biology","author":"Tannous","year":"2015"},{"key":"bib46","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1038\/nprot.2006.37","type":"journal-article","article-title":"Production and purification of lentiviral vectors","volume":"1","author":"Tiscornia","year":"2006","journal-title":"Nature Protocols"},{"key":"bib47","doi-asserted-by":"publisher","first-page":"3850","DOI":"10.1073\/pnas.1117963109","type":"journal-article","article-title":"Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility","volume":"109","author":"Tokuhiro","year":"2012","journal-title":"PNAS"},{"key":"bib48","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1095\/biolreprod47.6.1161","type":"journal-article","article-title":"Masking the cryptodeterminant on the 54-kilodalton mouse sperm surface antigen","volume":"47","author":"Toshimori","year":"1992","journal-title":"Biology of Reproduction"},{"key":"bib49","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1262\/jrd1955.16.152","type":"journal-article","article-title":"Studies on the fertilization of mouse eggs in vitro","volume":"16","author":"Toyoda","year":"1971","journal-title":"Jpn. J. Anim. Reprod"},{"key":"bib50","doi-asserted-by":"publisher","first-page":"W401","DOI":"10.1093\/nar\/gkv485","type":"journal-article","article-title":"The TOPCONS web server for consensus prediction of membrane protein topology and signal peptides","volume":"43","author":"Tsirigos","year":"2015","journal-title":"Nucleic Acids Research"},{"key":"bib51","doi-asserted-by":"publisher","first-page":"1033","DOI":"10.1093\/biolre\/ioaa018","type":"journal-article","article-title":"LYPD4, mouse homolog of a human acrosome protein, is essential for sperm fertilizing ability and male fertility\u2020","volume":"102","author":"Wang","year":"2020","journal-title":"Biology of Reproduction"},{"key":"bib52","doi-asserted-by":"publisher","first-page":"501","DOI":"10.1093\/nar\/gkac633","type":"journal-article","article-title":"Construction of a cross-species cell landscape at single-cell level","volume":"51","author":"Wang","year":"2023","journal-title":"Nucleic Acids Research"},{"key":"bib53","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1011337","type":"journal-article","article-title":"Golgi associated RAB2 interactor protein family contributes to murine male fertility to various extents by assuring correct morphogenesis of sperm heads","volume":"20","author":"Wang","year":"2024","journal-title":"PLOS Genetics"},{"key":"bib54","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1095\/biolreprod.106.052977","type":"journal-article","article-title":"Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice","volume":"75","author":"Yamaguchi","year":"2006","journal-title":"Biology of Reproduction"},{"key":"bib55","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1095\/biolreprod.108.074021","type":"journal-article","article-title":"Disruption of ADAM3 impairs the migration of sperm into oviduct in mouse","volume":"81","author":"Yamaguchi","year":"2009","journal-title":"Biology of Reproduction"},{"key":"bib56","doi-asserted-by":"publisher","first-page":"11211","DOI":"10.1073\/pnas.162027899","type":"journal-article","article-title":"Lack of acrosome formation in mice lacking a Golgi protein, GOPC","volume":"99","author":"Yao","year":"2002","journal-title":"PNAS"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/107494\/elife-107494-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/107494\/elife-107494-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/107494","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:18:53Z","timestamp":1787563133000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/107494"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,11,12]]},"references-count":56,"alternative-id":["10.7554\/eLife.107494"],"URL":"https:\/\/doi.org\/10.7554\/elife.107494","archive":["CLOCKSS"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2025.05.12.653469","asserted-by":"subject"},{"id-type":"doi","id":"10.1101\/2025.05.12.653469","asserted-by":"object"}],"is-same-as":[{"id-type":"doi","id":"10.7554\/eLife.107494.3","asserted-by":"subject"}],"is-version-of":[{"id-type":"doi","id":"10.7554\/eLife.107494.1","asserted-by":"subject"},{"id-type":"doi","id":"10.7554\/eLife.107494.2","asserted-by":"subject"}],"is-financed-by":[{"id-type":"doi","id":"10.52926\/jpmjfr211f","asserted-by":"subject"},{"id-type":"doi","id":"10.52926\/jpmjcr21n1","asserted-by":"subject"},{"id-type":"doi","id":"10.52926\/JPMJFR211F","asserted-by":"object"},{"id-type":"doi","id":"10.52926\/JPMJCR21N1","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,11,12]]},"assertion":[{"value":"single anonymised","name":"peer_review_transparency","label":"Peer review transparency","group":{"name":"peer_review_taxonomy"}},{"value":"other reviewer(s), editor","name":"peer_review_interaction","label":"Peer review interaction","group":{"name":"peer_review_taxonomy"}},{"value":"review summaries, review reports, author\/editor communication, reviewer identities reviewer opt in, editor identities","name":"peer_review_published","label":"Peer review published","group":{"name":"peer_review_taxonomy"}},{"value":"open (sign in with ORCID iD required)","name":"post_publication_commenting","label":"Post publication commenting","group":{"name":"post_publication_commenting"}}],"article-number":"RP107494"}}