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Thus, modification of the native airway community may serve as a means of altering the local environment in favor of health. In this work, we hypothesize that synthetic bacterial communities introduced into the airway can serve as prophylactic countermeasures against infection by\n                    <jats:italic>Burkholderia thailandensis<\/jats:italic>\n                    in mice. We demonstrate that understanding of antagonistic interactions between a pathogen and airway microbiota in vitro can guide identification of probiotics with protective capabilities in vivo. Specifically, we show that niche overlap between the probiotic and pathogen is indicative of probiotic performance in vivo. This work serves as a foundation for the rational design of probiotic communities for protection against and treatment of respiratory infections.\n                  <\/jats:p>","DOI":"10.7554\/elife.108304","type":"journal-article","created":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T15:30:08Z","timestamp":1765899008000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.108304","source":"Crossref","is-referenced-by-count":0,"title":["Niche exclusion of a lung pathogen in mice with designed probiotic communities"],"prefix":"10.7554","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0256-4507","authenticated-orcid":true,"given":"Kelsey E","family":"Hern","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01an7q238","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant and Microbial Biology, University of California, Berkeley","place":["Berkeley, United 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