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In comparison to viral infections, Mtb antigens are relatively limited and hence, challenging to sample. Specialized antigen presentation pathways enable the presentation of such scarce antigens to CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, which are, thus, uniquely poised to survey intracellular environments. A subset of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells prevalent in the airways, known as mucosal-associated invariant T (MAIT) cells, can be activated through the presentation of Mtb antigens via the major histocompatibility complex class I-related protein 1 (MR1) molecule. Prior work demonstrates that endosomal calcium signaling is critical for MR1-mediated presentation of Mtb-derived antigens. Here, we show that the calcium-sensing trafficking proteins Synaptotagmin (Syt) 1 and Syt7 specifically promote MAIT cell activation in response to Mtb-infected cells. In bronchial epithelial cells, Syt1 and Syt7 localize to late endo-lysosomes and MR1 vesicles. Loss of Syt1 and Syt7 results in enlarged MR1 vesicles and an increased number of MR1 vesicles in close proximity to Mtb-containing vacuoles during infection. This study identifies a specialized pathway in which Syt1 and Syt7 facilitate the translocation of MR1 from Mtb-containing vacuoles, potentially to the cell surface for antigen presentation.\n                  <\/jats:p>","DOI":"10.7554\/elife.108318","type":"journal-article","created":{"date-parts":[[2025,9,24]],"date-time":"2025-09-24T16:30:10Z","timestamp":1758731410000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.108318","source":"Crossref","is-referenced-by-count":0,"title":["Synaptotagmin 1 and Synaptotagmin 7 promote MR1-mediated presentation of Mycobacterium tuberculosis antigens"],"prefix":"10.7554","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6704-4644","authenticated-orcid":true,"given":"Se-Jin","family":"Kim","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/009avj582","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Pulmonary, Allergy, and Critical Care Medicine, Oregon Health and Science 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