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While these processes are known to interact at a coarse scale, the specific mechanisms integrating them have remained elusive. Using large-scale, wide-field calcium imaging, high-density multielectrode array recordings, single-cell RNA sequencing, and immunohistochemistry, we characterise a tightly aligned centrifugal expansion pattern during retinal development. This pattern is common to stage II retinal wave onsets, vascular development, Heme oxygenase-1 (Hmox1) expressing microglia, apoptotic cell markers, and a novel set of auto-fluorescent cluster complexes (ACCs) identified in this study. Apoptotic cells are known to upregulate functional pannexin-1 (PANX-1) hemichannels. These voltage-gated channels release purinergic molecules which act as \u2018eat me\u2019 signals to neighbouring microglia. PANX-1 hemichannel blockade with the drug probenecid results in a profound decrease in spontaneous wave frequency and strength, suggesting that retinal waves are indeed triggered by these apoptotic cells. Taken together, our observations suggest that spontaneous waves are initially triggered in hotspots by hyperactive apoptotic retinal ganglion cells (RGCs) in unvascularised retinal areas. These apoptotic cells release purinergic molecules via PANX-1 hemichannels, leading to wave generation. This hyperactivity leads to local hypoxic conditions, which, coupled with high extracellular ATP concentrations, promotes angiogenesis. Once blood vessels reach a particular hotspot, ATP release activates Hmox1-positive microglia, which engulf the dying RGCs, creating the auto-fluorescent clusters. Herein, we present a unified mechanism linking causally linking early neural activity, programmed cell death, and angiogenesis in the mammalian retina.<\/jats:p>","DOI":"10.7554\/elife.111419","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:30:14Z","timestamp":1780929014000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.111419","source":"Crossref","is-referenced-by-count":0,"title":["Developmental synchrony of retinal waves, apoptosis, and angiogenesis in postnatal retina"],"prefix":"10.7554","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2717-5412","authenticated-orcid":true,"given":"Michael A","family":"Savage","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01kj2bm70","id-type":"ROR","asserted-by":"publisher"}],"name":"Biosciences Institute, Faculty of Medical Sciences, Newcastle University","place":["Newcastle upon Tyne, United 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