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A well-known example is the specialized association of\n                    <jats:italic>Drosophila sechellia<\/jats:italic>\n                    with noni fruit (\n                    <jats:italic>Morinda citrifolia<\/jats:italic>\n                    ), which is toxic to other insects, including\n                    <jats:italic>Drosophila simulans<\/jats:italic>\n                    and\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    . The main noni toxin is octanoic acid (OA), but the mechanisms that determine sensitivity or resistance to OA in different species remain unclear. Here, we experimentally evolved\n                    <jats:italic>D. simulans<\/jats:italic>\n                    with increased OA resistance, identifying multiple loci under selection. Cross-referencing these with a genome-wide, OA resistance CRISPR screen in a\n                    <jats:italic>D. melanogaster<\/jats:italic>\n                    cell line highlighted two proteins: Kraken, a putative detoxification enzyme expressed in digestive and renal tissues, and Alkbh7, a mitochondrial protein linked to fatty acid metabolism. Both genes show elevated expression in\n                    <jats:italic>D. sechellia<\/jats:italic>\n                    and OA-resistant\n                    <jats:italic>D. simulans<\/jats:italic>\n                    . In\n                    <jats:italic>D. melanogaster<\/jats:italic>\n                    ,\n                    <jats:italic>kraken<\/jats:italic>\n                    mutants are more OA-sensitive, while\n                    <jats:italic>Alkbh7<\/jats:italic>\n                    overexpression increased OA resistance. Mutation of these genes in\n                    <jats:italic>D. sechellia<\/jats:italic>\n                    reduced OA tolerance. Our identification of genes contributing to OA resistance in laboratory and natural contexts demonstrates how complementary selection approaches can provide insights into complex mechanisms of toxin susceptibility and adaptation. Such methods could have practical applications in the characterization of natural and artificial insecticides.\n                  <\/jats:p>","DOI":"10.7554\/elife.111773","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:48:33Z","timestamp":1782917313000},"update-policy":"https:\/\/doi.org\/10.7554\/elife.111773","source":"Crossref","is-referenced-by-count":0,"title":["Intersecting experimental evolution and CRISPR screens to identify novel toxin resistance loci"],"prefix":"10.7554","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6785-9106","authenticated-orcid":true,"given":"Michele","family":"Marconcini","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/019whta54","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Integrative Genomics, Faculty of Biology and Medicine, University of Lausanne","place":["Lausanne, 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