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Currently, little data exist pertaining to peptides that are presented after T. gondii infection. Herein we purify HLA-A*02:01 complexes from T. gondii infected cells and characterize the peptide ligands using LCMS. We identify 195 T. gondii encoded ligands originating from both secreted and cytoplasmic proteins. Surprisingly, T. gondii ligands are significantly longer than uninfected host ligands, and these longer pathogen-derived peptides maintain a canonical N-terminal binding core yet exhibit a C-terminal extension of 1\u201330 amino acids. Structural analysis demonstrates that binding of extended peptides opens the HLA class I F\u2019 pocket, allowing the C-terminal extension to protrude through one end of the binding groove. In summary, we demonstrate that unrealized structural flexibility makes MHC class I receptive to parasite-derived ligands that exhibit unique C-terminal peptide extensions.<\/jats:p>","DOI":"10.7554\/elife.12556","type":"journal-article","created":{"date-parts":[[2016,1,29]],"date-time":"2016-01-29T07:35:28Z","timestamp":1454052928000},"source":"Crossref","is-referenced-by-count":81,"title":["Toxoplasma gondii peptide ligands open the gate of the HLA class I binding groove"],"prefix":"10.7554","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4540-4140","authenticated-orcid":true,"given":"Curtis","family":"McMurtrey","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, United States"},{"name":"Pure MHC LLC, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0762-2198","authenticated-orcid":true,"given":"Thomas","family":"Trolle","sequence":"additional","affiliation":[{"name":"Center for Biological Sequence Analysis, Technical University of Denmark, Kongens Lyngby, Denmark"},{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Sansom","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University at Buffalo School of Medicine, Buffalo, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soumya G","family":"Remesh","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Kaever","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried","family":"Bardet","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth","family":"Jackson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rima","family":"McLeod","sequence":"additional","affiliation":[{"name":"University of Chicago, Chicago, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morten","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Center for Biological Sequence Analysis, Technical University of Denmark, Kongens Lyngby, Denmark"},{"name":"Instituto de Investigaciones Biotecnol\u00f3gicas, Universidad Nacional de San Mart\u00edn, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk M","family":"Zajonc","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ira J","family":"Blader","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University at Buffalo School of Medicine, Buffalo, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bjoern","family":"Peters","sequence":"additional","affiliation":[{"name":"La Jolla Institute for Allergy and Immunology, La Jolla, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Hildebrand","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City, United States"},{"name":"Pure MHC LLC, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2016,1,29]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"246","DOI":"10.1002\/ijc.27661","article-title":"Molecular characterisation of the monocytic cell line THP-1 demonstrates a discrepancy with the documented HLA type","volume":"132","author":"Battle","year":"2013","journal-title":"International Journal of Cancer. 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