{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:28:16Z","timestamp":1790836096257,"version":"4.1.0"},"reference-count":45,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T00:00:00Z","timestamp":1468886400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005304","name":"Agence Nationale pour le D\u00e9veloppement de la Recherche en Sant\u00e9","doi-asserted-by":"publisher","award":["ANR12-BSV4-0011-01"],"award-info":[{"award-number":["ANR12-BSV4-0011-01"]}],"id":[{"id":"10.13039\/501100005304","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Breathing is a vital rhythmic behavior generated by hindbrain neuronal circuitry, including the preB\u00f6tzinger complex network (preB\u00f6tC) that controls inspiration. The emergence of preB\u00f6tC network activity during prenatal development has been described, but little is known regarding inspiratory neurons expressing pacemaker properties at embryonic stages. Here, we combined calcium imaging and electrophysiological recordings in mouse embryo brainstem slices together with computational modeling to reveal the existence of heterogeneous pacemaker oscillatory properties relying on distinct combinations of burst-generating INaP and ICAN conductances. The respective proportion of the different inspiratory pacemaker subtypes changes during prenatal development. Concomitantly, network rhythmogenesis switches from a purely INaP\/ICAN-dependent mechanism at E16.5 to a combined pacemaker\/network-driven process at E18.5. Our results provide the first description of pacemaker bursting properties in embryonic preB\u00f6tC neurons and indicate that network rhythmogenesis undergoes important changes during prenatal development through alterations in both circuit properties and the biophysical characteristics of pacemaker neurons.<\/jats:p>","DOI":"10.7554\/elife.16125","type":"journal-article","created":{"date-parts":[[2016,7,19]],"date-time":"2016-07-19T07:59:42Z","timestamp":1468915182000},"source":"Crossref","is-referenced-by-count":42,"title":["Development of pacemaker properties and rhythmogenic mechanisms in the mouse embryonic respiratory network"],"prefix":"10.7554","volume":"5","author":[{"given":"Marc","family":"Chevalier","sequence":"first","affiliation":[{"name":"Institut de Neurosciences Cognitives et Int\u00e9gratives d'Aquitaine, CNRS UMR 5287, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalia","family":"Toporikova","sequence":"additional","affiliation":[{"name":"Department of Biology, Washington and Lee University, Lexington, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Simmers","sequence":"additional","affiliation":[{"name":"Institut de Neurosciences Cognitives et Int\u00e9gratives d'Aquitaine, CNRS UMR 5287, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3214-1724","authenticated-orcid":true,"given":"Muriel","family":"Thoby-Brisson","sequence":"additional","affiliation":[{"name":"Institut de Neurosciences Cognitives et Int\u00e9gratives d'Aquitaine, CNRS UMR 5287, Universit\u00e9 de Bordeaux, Bordeaux, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2016,7,19]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"510","DOI":"10.1111\/j.1460-9568.2008.06345.x","article-title":"Brain-derived neurotrophic factor enhances fetal respiratory rhythm frequency in the mouse preB\u00f6tzinger complex in vitro","volume":"28","author":"Bouvier","year":"2008","journal-title":"European Journal of Neuroscience"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1066","DOI":"10.1038\/nn.2622","article-title":"Hindbrain interneurons and axon guidance signaling critical for breathing","volume":"13","author":"Bouvier","year":"2010","journal-title":"Nature Neuroscience"},{"key":"bib3","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1152\/jn.1999.82.1.382","article-title":"Models of respiratory rhythm generation in the pre-B\u00f6tzinger complex. 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