{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T11:58:27Z","timestamp":1789387107121,"version":"build-2803163510"},"reference-count":62,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T00:00:00Z","timestamp":1493078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T00:00:00Z","timestamp":1493078400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,4,25]],"date-time":"2017-04-25T00:00:00Z","timestamp":1493078400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The nicotinic acetylcholine receptor (nAChR) is a major target of autoantibodies in myasthenia gravis (MG), an autoimmune disease that causes neuromuscular transmission dysfunction. Despite decades of research, the molecular mechanisms underlying MG have not been fully elucidated. Here, we present the crystal structure of the nAChR \u03b11 subunit bound by the Fab fragment of mAb35, a reference monoclonal antibody that causes experimental MG and competes with ~65% of antibodies from MG patients. Our structures reveal for the first time the detailed molecular interactions between MG antibodies and a core region on nAChR \u03b11. These structures suggest a major nAChR-binding mechanism shared by a large number of MG antibodies and the possibility to treat MG by blocking this binding mechanism. Structure-based modeling also provides insights into antibody-mediated nAChR cross-linking known to cause receptor degradation. Our studies establish a structural basis for further mechanistic studies and therapeutic development of MG.<\/jats:p>","DOI":"10.7554\/elife.23043","type":"journal-article","created":{"date-parts":[[2017,4,21]],"date-time":"2017-04-21T03:00:24Z","timestamp":1492743624000},"source":"Crossref","is-referenced-by-count":33,"title":["Structural insights into the molecular mechanisms of myasthenia gravis and their therapeutic implications"],"prefix":"10.7554","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4303-7511","authenticated-orcid":true,"given":"Kaori","family":"Noridomi","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Southern California, Los Angeles, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3911-3021","authenticated-orcid":true,"given":"Go","family":"Watanabe","sequence":"additional","affiliation":[{"name":"USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa N","family":"Hansen","sequence":"additional","affiliation":[{"name":"Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gye Won","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Bridge Institute, University of Southern California, Los Angeles, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4798-6199","authenticated-orcid":true,"given":"Lin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Southern California, Los Angeles, United States"},{"name":"USC Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, United States"},{"name":"Molecular and Computational Biology, Department of Biological Sciences, University of Southern California, Los Angeles, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2017,4,25]]},"reference":[{"key":"bib1","doi-asserted-by":"crossref","first-page":"5916","DOI":"10.1016\/S0021-9258(18)60652-0","article-title":"Monoclonal antibodies to the main immunogenic region of the nicotinic acetylcholine receptor bind to residues 61-76 of the alpha subunit","volume":"263","author":"Barkas","year":"1988","journal-title":"The Journal of Biological Chemistry"},{"key":"bib2","doi-asserted-by":"crossref","first-page":"3568","DOI":"10.4049\/jimmunol.143.11.3568","article-title":"The main immunogenic region of the nicotinic acetylcholine receptor. identification of amino acid residues interacting with different antibodies","volume":"143","author":"Bellone","year":"1989","journal-title":"Journal of Immunology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"323","DOI":"10.1016\/0896-6273(95)90037-3","article-title":"Three-dimensional location of the main immunogenic region of the acetylcholine receptor","volume":"15","author":"Beroukhim","year":"1995","journal-title":"Neuron"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1006\/jaut.1995.0011","article-title":"Myasthenia gravis, a model of organ-specific autoimmune disease","volume":"8","author":"Berrih-Aknin","year":"1995","journal-title":"Journal of Autoimmunity"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"557","DOI":"10.1113\/jphysiol.2009.184085","article-title":"In pursuit of the high-resolution structure of nicotinic acetylcholine receptors","volume":"588","author":"Chen","year":"2010","journal-title":"The Journal of Physiology"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1107\/S0907444994003112","article-title":"The CCP4 suite: programs for protein crystallography","volume":"50","author":"Collaborative Computational Project Number 4","year":"1994","journal-title":"Acta Crystallographica. 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