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Its polymerization kinetics are unusual, permitting only short, unstable F-actin filaments. It has not been possible to study actin in vivo and so its physiological roles have remained obscure, leading to models distinct from conventional actin behaviour. Here a modified version of the commercially available actin-chromobody was tested as a novel tool for visualising F-actin dynamics in Toxoplasma gondii. Cb labels filamentous actin structures within the parasite cytosol and labels an extensive F-actin network that connects parasites within the parasitophorous vacuole and allows vesicles to be exchanged between parasites. In the absence of actin, parasites lack a residual body and inter-parasite connections and grow in an asynchronous and disorganized manner. Collectively, these data identify new roles for actin in the intracellular phase of the parasites lytic cycle and provide a robust new tool for imaging parasitic F-actin dynamics.<\/jats:p>","DOI":"10.7554\/elife.24119","type":"journal-article","created":{"date-parts":[[2017,3,20]],"date-time":"2017-03-20T20:00:14Z","timestamp":1490040014000},"source":"Crossref","is-referenced-by-count":136,"title":["Toxoplasma gondii F-actin forms an extensive filamentous network required for material exchange and parasite maturation"],"prefix":"10.7554","volume":"6","author":[{"given":"Javier","family":"Periz","sequence":"first","affiliation":[{"name":"Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity & Inflammation, University of Glasgow, Glasgow, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jamie","family":"Whitelaw","sequence":"additional","affiliation":[{"name":"Wellcome Centre for Molecular Parasitology, Institute of Infection, 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