{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T14:49:49Z","timestamp":1792853389469,"version":"4.5.0"},"reference-count":78,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,11,24]],"date-time":"2017-11-24T00:00:00Z","timestamp":1511481600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DEB-1354625"],"award-info":[{"award-number":["DEB-1354625"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003151","name":"Fonds de Recherche du Qu\u00e9bec - Nature et Technologies","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Intracellular parasitism often results in gene loss, genome reduction, and dependence upon the host for cellular functioning. Rozellomycota is a clade comprising many such parasites and is related to the diverse, highly reduced, animal parasites, Microsporidia. We sequenced the nuclear and mitochondrial genomes of Paramicrosporidium saccamoebae [Rozellomycota], an intranuclear parasite of amoebae. A canonical fungal mitochondrial genome was recovered from P. saccamoebae that encodes genes necessary for the complete oxidative phosphorylation pathway including Complex I, differentiating it from most endoparasites including its sequenced relatives in Rozellomycota and Microsporidia. Comparative analysis revealed that P. saccamoebae shares more gene content with distantly related Fungi than with its closest relatives, suggesting that genome evolution in Rozellomycota and Microsporidia has been affected by repeated and independent gene losses, possibly as a result of variation in parasitic strategies (e.g. host and subcellular localization) or due to multiple transitions to parasitism.<\/jats:p>","DOI":"10.7554\/elife.29594","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T11:00:15Z","timestamp":1511434815000},"source":"Crossref","is-referenced-by-count":81,"title":["The genome of an intranuclear parasite, Paramicrosporidium saccamoebae, reveals alternative adaptations to obligate intracellular parasitism"],"prefix":"10.7554","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0260-8995","authenticated-orcid":true,"given":"C Alisha","family":"Quandt","sequence":"first","affiliation":[{"name":"Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denis","family":"Beaudet","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, Ottawa, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Corsaro","sequence":"additional","affiliation":[{"name":"CHLAREAS Chlamydia Research Association, Nancy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Walochnik","sequence":"additional","affiliation":[{"name":"Molecular Parasitology, Institute for Specific Prophylaxis and Tropical Medicine, Medical University of Vienna, Koblenz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Michel","sequence":"additional","affiliation":[{"name":"Laboratory of Electron Microscopy, Central Institute of the Federal Armed Forces Medical Services, Koblenz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7932-7932","authenticated-orcid":true,"given":"Nicolas","family":"Corradi","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Ottawa, Ottawa, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1123-5986","authenticated-orcid":true,"given":"Timothy Y","family":"James","sequence":"additional","affiliation":[{"name":"Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2017,11,24]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1093\/gbe\/evu028","article-title":"High variability of mitochondrial gene order among fungi","volume":"6","author":"Aguileta","year":"2014","journal-title":"Genome Biology and Evolution"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"4116","DOI":"10.1073\/pnas.1517242113","article-title":"Horizontally acquired genes in early-diverging pathogenic fungi enable the use of host nucleosides and nucleotides","volume":"113","author":"Alexander","year":"2016","journal-title":"PNAS"},{"key":"bib3","article-title":"Scripts required to calculate tetramer frequencies and create input files for ESOM","volume-title":"Github","author":"Anderson","year":"2010","unstructured":"Anderson A, Itai S, Jain S. 2010. 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