{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T10:04:39Z","timestamp":1790762679005,"version":"4.1.0"},"reference-count":41,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T00:00:00Z","timestamp":1515024000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/K01207X\/1"],"award-info":[{"award-number":["MR\/K01207X\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/K020196\/1"],"award-info":[{"award-number":["MR\/K020196\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000848","name":"University Of Edinburgh","doi-asserted-by":"publisher","award":["Principal Career Development PhD Scholarship"],"award-info":[{"award-number":["Principal Career Development PhD Scholarship"]}],"id":[{"id":"10.13039\/501100000848","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","award":["MR\/L008076\/1"],"award-info":[{"award-number":["MR\/L008076\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Both TH2-dependent helminth killing and suppression of the TH2 effector response have been attributed to macrophages (M\u03a6) activated by IL-4 (M(IL-4)). To investigate how M(IL-4) contribute to diverse infection outcomes, the M\u03a6 compartment of susceptible BALB\/c mice and more resistant C57BL\/6 mice was profiled during infection of the pleural cavity with the filarial nematode, Litomosoides sigmodontis. C57BL\/6 mice exhibited a profoundly expanded resident M\u03a6 (resM\u03a6) population, which was gradually replenished from the bone marrow in an age-dependent manner. Infection status did not alter the bone-marrow derived contribution to the resM\u03a6 population, confirming local proliferation as the driver of resM\u03a6 expansion. Significantly less resM\u03a6 expansion was observed in the susceptible BALB\/c strain, which instead exhibited an influx of monocytes that assumed an immunosuppressive PD-L2+ phenotype. Inhibition of monocyte recruitment enhanced nematode killing. Thus, the balance of monocytic vs. resident M(IL-4) numbers varies between inbred mouse strains and impacts infection outcome.<\/jats:p>","DOI":"10.7554\/elife.30947","type":"journal-article","created":{"date-parts":[[2018,1,4]],"date-time":"2018-01-04T06:00:51Z","timestamp":1515045651000},"source":"Crossref","is-referenced-by-count":50,"title":["Myeloid cell recruitment versus local proliferation differentiates susceptibility from resistance to filarial infection"],"prefix":"10.7554","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5558-0749","authenticated-orcid":true,"given":"Sharon M","family":"Campbell","sequence":"first","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9038-0566","authenticated-orcid":true,"given":"Johanna A","family":"Knipper","sequence":"additional","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0206-1451","authenticated-orcid":true,"given":"Dominik","family":"Ruckerl","sequence":"additional","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"},{"name":"Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, Faculty of Biology, Medicine & Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Conor M","family":"Finlay","sequence":"additional","affiliation":[{"name":"Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, Faculty of Biology, Medicine & Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Logan","sequence":"additional","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9663-1928","authenticated-orcid":true,"given":"Carlos M","family":"Minutti","sequence":"additional","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Mack","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine II, University Hospital Regensburg, Regensburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J","family":"Jenkins","sequence":"additional","affiliation":[{"name":"Centre for Inflammation Research, School of Clinical Sciences, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew D","family":"Taylor","sequence":"additional","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3829-066X","authenticated-orcid":true,"given":"Judith E","family":"Allen","sequence":"additional","affiliation":[{"name":"Centre for Immunity, Infection and Evolution, School of Biological Sciences, University of Edinburgh, Edinburgh, United Kingdom"},{"name":"Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, Faculty of Biology, Medicine & Health, Manchester Academic Health Science Centre, University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2018,1,4]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"955","DOI":"10.1038\/nm1451","article-title":"Memory T(H)2 cells induce alternatively activated macrophages to mediate protection against nematode parasites","volume":"12","author":"Anthony","year":"2006","journal-title":"Nature Medicine"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"6820","DOI":"10.1128\/IAI.71.12.6820-6829.2003","article-title":"Resistance and susceptibility to filarial infection with Litomosoides sigmodontis are associated with early differences in parasite development and in localized immune reactions","volume":"71","author":"Babayan","year":"2003","journal-title":"Infection and Immunity"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"ncomms11852","DOI":"10.1038\/ncomms11852","article-title":"Long-lived self-renewing bone marrow-derived macrophages displace embryo-derived cells to inhabit adult serous cavities","volume":"7","author":"Bain","year":"2016","journal-title":"Nature Communications"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.immuni.2017.07.019","article-title":"T cell zone resident macrophages silently dispose of apoptotic cells in the lymph node","volume":"47","author":"Baratin","year":"2017","journal-title":"Immunity"},{"key":"bib5","doi-asserted-by":"publisher","first-page":"3346","DOI":"10.1128\/IAI.00625-13","article-title":"Human and mouse macrophages collaborate with neutrophils to kill larval Strongyloides stercoralis","volume":"81","author":"Bonne-Ann\u00e9e","year":"2013","journal-title":"Infection and Immunity"},{"key":"bib6","doi-asserted-by":"publisher","first-page":"5116","DOI":"10.4049\/jimmunol.178.8.5116","article-title":"Mannose-binding lectin A-deficient mice have abrogated antigen-specific IgM responses and increased susceptibility to a nematode infection","volume":"178","author":"Carter","year":"2007","journal-title":"The Journal of Immunology"},{"key":"bib7","doi-asserted-by":"publisher","first-page":"2155","DOI":"10.1002\/eji.201141817","article-title":"A quantifiable proliferative burst of tissue macrophages restores homeostatic macrophage populations after acute inflammation","volume":"41","author":"Davies","year":"2011","journal-title":"European Journal of Immunology"},{"key":"bib8","doi-asserted-by":"publisher","first-page":"1441","DOI":"10.1002\/eji.1830240630","article-title":"Interleukin-13 alters the activation state of murine macrophages in vitro: comparison with interleukin-4 and interferon-gamma","volume":"24","author":"Doyle","year":"1994","journal-title":"European Journal of Immunology"},{"key":"bib9","doi-asserted-by":"publisher","first-page":"e1003771","DOI":"10.1371\/journal.ppat.1003771","article-title":"Antibodies trap tissue migrating helminth larvae and prevent tissue damage by driving IL-4R\u03b1-independent alternative differentiation of macrophages","volume":"9","author":"Esser-von Bieren","year":"2013","journal-title":"PLoS Pathogens"},{"key":"bib10","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1126\/science.1194637","article-title":"Fate mapping analysis reveals that adult microglia derive from primitive macrophages","volume":"330","author":"Ginhoux","year":"2010","journal-title":"Science"},{"key":"bib11","doi-asserted-by":"publisher","first-page":"612","DOI":"10.1016\/j.micinf.2004.12.019","article-title":"Quantitative appraisal of murine filariasis confirms host strain differences but reveals that BALB\/c females are more susceptible than males to Litomosoides sigmodontis","volume":"7","author":"Graham","year":"2005","journal-title":"Microbes and Infection"},{"key":"bib12","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1038\/ni.3734","article-title":"Vitamin A mediates conversion of monocyte-derived macrophages into tissue-resident macrophages during alternative activation","volume":"18","author":"Gundra","year":"2017","journal-title":"Nature Immunology"},{"key":"bib13","doi-asserted-by":"publisher","first-page":"e110","DOI":"10.1182\/blood-2013-08-520619","article-title":"Alternatively activated macrophages derived from monocytes and tissue macrophages are phenotypically and functionally distinct","volume":"123","author":"Gundra","year":"2014","journal-title":"Blood"},{"key":"bib14","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1016\/S0169-4758(00)01738-5","article-title":"Litomosoides sigmodontis in mice: reappraisal of an old model for filarial research","volume":"16","author":"Hoffmann","year":"2000","journal-title":"Parasitology Today"},{"key":"bib15","doi-asserted-by":"publisher","first-page":"12651","DOI":"10.1038\/ncomms12651","article-title":"Fat-associated lymphoid clusters control local IgM secretion during pleural infection and lung inflammation","volume":"7","author":"Jackson-Jones","year":"2016","journal-title":"Nature Communications"},{"key":"bib16","doi-asserted-by":"publisher","first-page":"1284","DOI":"10.1126\/science.1204351","article-title":"Local macrophage proliferation, rather than recruitment from the blood, is a signature of TH2 inflammation","volume":"332","author":"Jenkins","year":"2011","journal-title":"Science"},{"key":"bib17","doi-asserted-by":"publisher","first-page":"2477","DOI":"10.1084\/jem.20121999","article-title":"IL-4 directly signals tissue-resident macrophages to proliferate beyond homeostatic levels controlled by CSF-1","volume":"210","author":"Jenkins","year":"2013","journal-title":"The Journal of Experimental Medicine"},{"key":"bib18","doi-asserted-by":"publisher","first-page":"1277","DOI":"10.1016\/S0020-7519(02)00125-X","article-title":"IL-4 is required to prevent filarial nematode development in resistant but not susceptible strains of mice","volume":"32","author":"Le Goff","year":"2002","journal-title":"International Journal for Parasitology"},{"key":"bib19","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1186\/1471-2172-3-7","article-title":"IL-4 dependent alternatively-activated macrophages have a distinctive in vivo gene expression phenotype","volume":"3","author":"Loke","year":"2002","journal-title":"BMC Immunology"},{"key":"bib20","doi-asserted-by":"publisher","first-page":"4697","DOI":"10.4049\/jimmunol.166.7.4697","article-title":"Expression and characterization of the chemokine receptors CCR2 and CCR5 in mice","volume":"166","author":"Mack","year":"2001","journal-title":"The Journal of Immunology"},{"key":"bib21","doi-asserted-by":"publisher","first-page":"380","DOI":"10.1165\/rcmb.2007-0224RC","article-title":"The prolonged life-span of alveolar macrophages","volume":"38","author":"Murphy","year":"2008","journal-title":"American Journal of Respiratory Cell and Molecular Biology"},{"key":"bib22","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1016\/j.immuni.2014.06.008","article-title":"Macrophage activation and polarization: nomenclature and experimental guidelines","volume":"41","author":"Murray","year":"2014","journal-title":"Immunity"},{"key":"bib23","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1084\/jem.20082048","article-title":"Alternatively activated macrophage-derived RELM-{alpha} is a negative regulator of type 2 inflammation in the lung","volume":"206","author":"Nair","year":"2009","journal-title":"The Journal of Experimental Medicine"},{"key":"bib24","doi-asserted-by":"publisher","first-page":"e1004282","DOI":"10.1371\/journal.ppat.1004282","article-title":"Ly6Chi monocyte recruitment is responsible for Th2 associated host-protective macrophage accumulation in liver inflammation due to schistosomiasis","volume":"10","author":"Nascimento","year":"2014","journal-title":"PLoS Pathogens"},{"key":"bib25","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1016\/j.cell.2014.04.016","article-title":"Tissue-specific signals control reversible program of localization and functional polarization of macrophages","volume":"157","author":"Okabe","year":"2014","journal-title":"Cell"},{"key":"bib26","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1038\/nri843","article-title":"The immunobiology of schistosomiasis","volume":"2","author":"Pearce","year":"2002","journal-title":"Nature Reviews Immunology"},{"key":"bib27","doi-asserted-by":"publisher","first-page":"e1000371","DOI":"10.1371\/journal.ppat.1000371","article-title":"Arginase-1-expressing macrophages suppress Th2 cytokine-driven inflammation and fibrosis","volume":"5","author":"Pesce","year":"2009","journal-title":"PLoS Pathogens"},{"key":"bib28","doi-asserted-by":"publisher","first-page":"e1000393","DOI":"10.1371\/journal.ppat.1000393","article-title":"Retnla (relmalpha\/fizz1) suppresses helminth-induced Th2-type immunity","volume":"5","author":"Pesce","year":"2009","journal-title":"PLoS Pathogens"},{"key":"bib29","doi-asserted-by":"publisher","first-page":"1827","DOI":"10.4049\/jimmunol.175.3.1827","article-title":"Critical role for IgM in host protection in experimental filarial infection","volume":"175","author":"Rajan","year":"2005","journal-title":"The Journal of Immunology"},{"key":"bib30","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1126\/science.1251414","article-title":"The transcription factor Gata6 links tissue macrophage phenotype and proliferative renewal","volume":"344","author":"Rosas","year":"2014","journal-title":"Science"},{"key":"bib31","doi-asserted-by":"crossref","first-page":"5519","DOI":"10.4049\/jimmunol.163.10.5519","article-title":"Defects in the generation of IFN-gamma are overcome to control infection with Leishmania donovani in CC chemokine receptor (CCR) 5-, macrophage inflammatory protein-1 alpha-, or CCR2-deficient mice","volume":"163","author":"Sato","year":"1999","journal-title":"Journal of Immunology"},{"key":"bib32","doi-asserted-by":"publisher","first-page":"86","DOI":"10.1126\/science.1219179","article-title":"A lineage of myeloid cells independent of Myb and hematopoietic stem cells","volume":"336","author":"Schulz","year":"2012","journal-title":"Science"},{"key":"bib33","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1084\/jem.176.1.287","article-title":"Interleukin 4 potently enhances murine macrophage mannose receptor activity: a marker of alternative immunologic macrophage activation","volume":"176","author":"Stein","year":"1992","journal-title":"Journal of Experimental Medicine"},{"key":"bib34","doi-asserted-by":"publisher","first-page":"4626","DOI":"10.4049\/jimmunol.179.7.4626","article-title":"CTLA-4 and CD4+ CD25+ regulatory T cells inhibit protective immunity to filarial parasites in vivo","volume":"179","author":"Taylor","year":"2007","journal-title":"The Journal of Immunology"},{"key":"bib35","doi-asserted-by":"publisher","first-page":"4924","DOI":"10.4049\/jimmunol.174.8.4924","article-title":"Removal of regulatory T cell activity reverses hyporesponsiveness and leads to filarial parasite clearance in vivo","volume":"174","author":"Taylor","year":"2005","journal-title":"The Journal of Immunology"},{"key":"bib36","doi-asserted-by":"publisher","first-page":"192","DOI":"10.1002\/eji.200838727","article-title":"Early recruitment of natural CD4+ Foxp3+ Treg cells by infective larvae determines the outcome of filarial infection","volume":"39","author":"Taylor","year":"2009","journal-title":"European Journal of Immunology"},{"key":"bib37","doi-asserted-by":"publisher","first-page":"40814","DOI":"10.1038\/srep40814","article-title":"Helminth-induced Ly6Chi monocyte-derived alternatively activated macrophages suppress experimental autoimmune encephalomyelitis","volume":"7","author":"Terrazas","year":"2017","journal-title":"Scientific Reports"},{"key":"bib38","doi-asserted-by":"publisher","first-page":"14693","DOI":"10.1038\/s41598-017-14935-3","article-title":"Ly6Chi inflammatory monocytes promote susceptibility to Leishmania donovani infection","volume":"7","author":"Terrazas","year":"2017","journal-title":"Scientific Reports"},{"key":"bib39","doi-asserted-by":"publisher","first-page":"e1003215","DOI":"10.1371\/journal.ppat.1003215","article-title":"Th2 cell-intrinsic hypo-responsiveness determines susceptibility to helminth infection","volume":"9","author":"van der Werf","year":"2013","journal-title":"PLoS Pathogens"},{"key":"bib40","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1016\/j.immuni.2012.12.001","article-title":"Fate mapping reveals origins and dynamics of monocytes and tissue macrophages under homeostasis","volume":"38","author":"Yona","year":"2013","journal-title":"Immunity"},{"key":"bib41","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1053\/j.gastro.2008.03.077","article-title":"Th2 cytokine-induced alterations in intestinal smooth muscle function depend on alternatively activated macrophages","volume":"135","author":"Zhao","year":"2008","journal-title":"Gastroenterology"}],"container-title":["eLife"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/cdn.elifesciences.org\/articles\/30947\/elife-30947-v1.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/cdn.elifesciences.org\/articles\/30947\/elife-30947-v1.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/elifesciences.org\/articles\/30947","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T20:48:16Z","timestamp":1697057296000},"score":1,"resource":{"primary":{"URL":"https:\/\/elifesciences.org\/articles\/30947"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,4]]},"references-count":41,"alternative-id":["10.7554\/eLife.30947"],"URL":"https:\/\/doi.org\/10.7554\/elife.30947","archive":["CLOCKSS"],"relation":{"has-review":[{"id-type":"doi","id":"10.7554\/eLife.30947.017","asserted-by":"object"},{"id-type":"doi","id":"10.7554\/eLife.30947.018","asserted-by":"object"}]},"ISSN":["2050-084X"],"issn-type":[{"value":"2050-084X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,4]]},"article-number":"e30947"}}