{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T12:27:24Z","timestamp":1787920044932,"version":"build-2784847793"},"reference-count":40,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T00:00:00Z","timestamp":1524096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T00:00:00Z","timestamp":1524096000000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"},{"start":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T00:00:00Z","timestamp":1524096000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/publicdomain\/zero\/1.0\/"}],"funder":[{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["Intramural Research Program Grant 1ZIADC000085-01"],"award-info":[{"award-number":["Intramural Research Program Grant 1ZIADC000085-01"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000055","name":"National Institute on Deafness and Other Communication Disorders","doi-asserted-by":"publisher","award":["Intramural Research Program Grant 1ZIADC000021"],"award-info":[{"award-number":["Intramural Research Program Grant 1ZIADC000021"]}],"id":[{"id":"10.13039\/100000055","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The orientation of hair bundles on top of sensory hair cells (HCs) in neuromasts of the lateral line system allows fish to detect direction of water flow. Each neuromast shows hair bundles arranged in two opposing directions and each afferent neuron innervates only HCs of the same orientation. Previously, we showed that this opposition is established by expression of Emx2 in half of the HCs, where it mediates hair bundle reversal (&lt;xref ref-type=\"bibr\" rid=\"bib15\"&gt;Jiang et al., 2017&lt;\/xref&gt;). Here, we show that Emx2 also regulates neuronal selection: afferent neurons innervate either Emx2-positive or negative HCs. In emx2 knockout and gain-of-function neuromasts, all HCs are unidirectional and the innervation patterns and physiological responses of the afferent neurons are dependent on the presence or absence of Emx2. Our results indicate that Emx2 mediates the directional selectivity of neuromasts by two distinct processes: regulating hair bundle orientation in HCs and selecting afferent neuronal targets.<\/jats:p>","DOI":"10.7554\/elife.35796","type":"journal-article","created":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T09:12:11Z","timestamp":1524129131000},"source":"Crossref","is-referenced-by-count":39,"title":["Directional selectivity of afferent neurons in zebrafish neuromasts is regulated by Emx2 in presynaptic hair cells"],"prefix":"10.7554","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8825-9783","authenticated-orcid":true,"given":"Young Rae","family":"Ji","sequence":"first","affiliation":[{"name":"Section on Sensory Cell Regeneration and Development, Laboratory of Molecular Biology, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunita","family":"Warrier","sequence":"additional","affiliation":[{"name":"Section on Sensory Cell Development and Function, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Jiang","sequence":"additional","affiliation":[{"name":"Section on Sensory Cell Regeneration and Development, Laboratory of Molecular Biology, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1400-3558","authenticated-orcid":true,"given":"Doris K","family":"Wu","sequence":"additional","affiliation":[{"name":"Section on Sensory Cell Regeneration and Development, Laboratory of Molecular Biology, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1065-8215","authenticated-orcid":true,"given":"Katie S","family":"Kindt","sequence":"additional","affiliation":[{"name":"Section on Sensory Cell Development and Function, National Institute on Deafness and Other Communication Disorders, National Institutes of Health, Bethesda, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2018,4,19]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"347","DOI":"10.1016\/S0166-2236(00)01608-8","article-title":"Emx homeogenes and mouse brain development","volume":"23","author":"Cecchi","year":"2000","journal-title":"Trends in Neurosciences"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0378-1119(02)00623-6","article-title":"Emx2: a gene responsible for cortical development, regionalization and area specification","volume":"291","author":"Cecchi","year":"2002","journal-title":"Gene"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1038\/264705a0","article-title":"Selective stabilisation of developing synapses as a mechanism for the specification of neuronal networks","volume":"264","author":"Changeux","year":"1976","journal-title":"Nature"},{"key":"bib4","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1007\/s003590050058","article-title":"Dipole source localization by the mottled sculpin. 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