{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:40:22Z","timestamp":1791355222390,"version":"4.3.1"},"reference-count":63,"publisher":"eLife Sciences Publications, Ltd","license":[{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T00:00:00Z","timestamp":1545091200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001465","name":"American Thoracic Society","doi-asserted-by":"publisher","award":["PCD\/Kovler Family foundation"],"award-info":[{"award-number":["PCD\/Kovler Family foundation"]}],"id":[{"id":"10.13039\/100001465","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["HL128370"],"award-info":[{"award-number":["HL128370"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Motile ciliopathies are characterized by specific defects in cilia beating that result in chronic airway disease, subfertility, ectopic pregnancy, and hydrocephalus. While many patients harbor mutations in the dynein motors that drive cilia beating, the disease also results from mutations in so-called dynein axonemal assembly factors (DNAAFs) that act in the cytoplasm. The mechanisms of DNAAF action remain poorly defined. Here, we show that DNAAFs concentrate together with axonemal dyneins and chaperones into organelles that form specifically in multiciliated cells, which we term DynAPs, for dynein axonemal particles. These organelles display hallmarks of biomolecular condensates, and remarkably, DynAPs are enriched for the stress granule protein G3bp1, but not for other stress granule proteins or P-body proteins. Finally, we show that both the formation and the liquid-like behaviors of DynAPs are disrupted in a model of motile ciliopathy. These findings provide a unifying cell biological framework for a poorly understood class of human disease genes and add motile ciliopathy to the growing roster of human diseases associated with disrupted biological phase separation.<\/jats:p>","DOI":"10.7554\/elife.38497","type":"journal-article","created":{"date-parts":[[2018,12,18]],"date-time":"2018-12-18T08:01:39Z","timestamp":1545120099000},"source":"Crossref","is-referenced-by-count":75,"title":["A liquid-like organelle at the root of motile ciliopathy"],"prefix":"10.7554","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4695-9674","authenticated-orcid":true,"given":"Ryan L","family":"Huizar","sequence":"first","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chanjae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7446-1120","authenticated-orcid":true,"given":"Alexander A","family":"Boulgakov","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5352-1948","authenticated-orcid":true,"given":"Amjad","family":"Horani","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Washington University School of Medicine, St Louis, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fan","family":"Tu","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8808-180X","authenticated-orcid":true,"given":"Edward M","family":"Marcotte","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven L","family":"Brody","sequence":"additional","affiliation":[{"name":"Department of Medicine, Washington University School of Medicine, St Louis, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6280-8625","authenticated-orcid":true,"given":"John B","family":"Wallingford","sequence":"additional","affiliation":[{"name":"Department of Molecular Biosciences, University of Texas, Austin, United States"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"4374","published-online":{"date-parts":[[2018,12,18]]},"reference":[{"key":"bib1","doi-asserted-by":"publisher","first-page":"4154","DOI":"10.1091\/mbc.e08-05-0513","article-title":"The dynamics of mammalian P body transport, assembly, and disassembly in vivo","volume":"19","author":"Aizer","year":"2008","journal-title":"Molecular Biology of the Cell"},{"key":"bib2","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1038\/nrm.2017.7","article-title":"Biomolecular condensates: organizers of cellular biochemistry","volume":"18","author":"Banani","year":"2017","journal-title":"Nature Reviews Molecular Cell Biology"},{"key":"bib3","doi-asserted-by":"publisher","first-page":"145","DOI":"10.1016\/j.devcel.2015.09.017","article-title":"Morpholinos: Antisense and Sensibility","volume":"35","author":"Blum","year":"2015","journal-title":"Developmental Cell"},{"key":"bib4","article-title":"FociFinder3D","volume-title":"Github","author":"Boulgakov","year":"2018","unstructured":"Boulgakov AA. 2018. 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